A5013428460- Computational Drug Discovery Methods
- Protein Structure and Dynamics
- Bioinformatics and Genomic Networks
- Gene Regulatory Network Analysis
- Carcinogens and Genotoxicity Assessment
- Metabolomics and Mass Spectrometry Studies
- Mental Health Research Topics
- Microbial Metabolic Engineering and Bioproduction
- Machine Learning in Bioinformatics
- Evolution and Genetic Dynamics
- Complex Systems and Time Series Analysis
- Glycosylation and Glycoproteins Research
- Gene expression and cancer classification
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- RNA and protein synthesis mechanisms
- Molecular spectroscopy and chirality
- Fractal and DNA sequence analysis
- Spectroscopy and Chemometric Analyses
- Functional Brain Connectivity Studies
- Free Radicals and Antioxidants
- Cell Image Analysis Techniques
- Ecosystem dynamics and resilience
- DNA Repair Mechanisms
- Enzyme Structure and Function
Istituto Superiore di Sanità
2016-2025
Agostino Gemelli University Polyclinic
2025
Università Cattolica del Sacro Cuore
2010-2025
University of Bologna
2001-2025
Roma Tre University
2023-2024
University of Perugia
2018-2023
McMaster University
2022
University of Cagliari
2022
Sapienza University of Rome
1989-2021
University of Malaya
2021
Cell fate choice and commitment of multipotent progenitor cells to a differentiated lineage requires broad changes their gene expression profile. But how overcome the stability configuration (attractor) exit attractor in one direction remains elusive. Here we show that blood erythroid or myeloid is preceded by destabilization high-dimensional state, such differentiating undergo critical state transition. Single-cell resolution analysis populations affords new quantitative index for...
8-Oxoguanine, a common mutagenic DNA lesion, generates G:C>T:A transversions via mispairing with adenine during replication. When operating normally, the MUTYH glycosylase prevents 8-oxoguanine-related mutagenesis by excising incorporated adenine. Biallelic mutations impair this enzymatic function and are associated colorectal cancer (CRC) in MUTYH-Associated Polyposis (MAP) syndrome. Here, we perform whole-exome sequencing that reveals modest mutator phenotype MAP CRCs compared to sporadic...
In postnatal tissues, angiogenesis occurs in nontumoral conditions on appropriate stimuli. the nervous tissue, hypoxia, neural graft, increased function, and synaptic activity are associated with neoangiogenesis. We have investigated occurrence of neoangiogenesis superior cervical ganglia (scg) newborn rats treated for 8–21 days 6-hydroxy-dopamine (6-OHDA), nerve growth factor (NGF), or 6-OHDA + NGF. The two latter treatments induced a significant increase scg size. However, after combined...
Oxidative stress contributes to the pathogenesis of aging-associated heart failure. Among various signaling pathways mediating oxidative stress, NAD(+) -dependent protein deacetylase SirT1 has been implicated in protection muscle. Expression a locally acting insulin-like growth factor-1 (IGF-1) propeptide (mIGF-1) helps recover from infarct and enhances expression cardiomyocytes (CM) vitro, exerting hypertrophic stresses. To study role mIGF-1/SirT1 vivo, we generated cardiac-specific mIGF-1...
The International Conference on Harmonization (ICH) M7 guideline allows the use of in silico approaches for predicting Ames mutagenicity initial assessment impurities pharmaceuticals. This is first international that addresses quantitative structure–activity relationship (QSAR) models lieu actual toxicological studies human health assessment. Therefore, QSAR now require higher predictive power identifying mutagenic chemicals. To increase models, larger experimental datasets from reliable...
MicroRNAs (miRNAs) play key roles in modulating a variety of cellular processes through repression mRNAs target. The functional relevance microRNAs has been proven normal and malignant hematopoiesis. While analyzing miRNAs expression profile unilineage serum-free liquid suspension cultures peripheral blood CD34+ hematopoietic progenitor cells (HPCs) the erythroid, megakaryocytic, granulocytic monocytic pathways, we identified miR-486-3p as mainly expressed within erythroid lineage. We showed...
Prostate Specific Antigen (PSA) fails to discriminate between benign prostatic hyperplasia (BPH) and Cancer (PCa), resulting in large numbers of unnecessary biopsies missed cancer diagnoses. Nanovesicles called exosomes are directly detectable patient plasma here we explore the potential use plasmatic expressing PSA (Exo-PSA) distinguishing healthy individuals, BPH, PCa. Exosomes were obtained from samples 80 PCa, donors (CTR). Nanoparticle Tracking Analysis (NTA), immunocapture-based ELISA...
SARS-CoV-2 has caused the largest pandemic of twenty-first century (COVID-19), threatening life and economy all countries in world. The identification novel therapies vaccines that can mitigate or control this global health threat is among most important challenges facing biomedical sciences. To construct a long-term strategy to fight both other possible future threats from coronaviruses, it critical understand molecular mechanisms underlying virus action. viral entry associated infectivity...
Quiescent/slow cycling cells have been identified in several tumors and correlated with therapy resistance. However, the features of chemoresistant populations molecular factors linking quiescence to chemoresistance are largely unknown.A population quiescent/slow was isolated through PKH26 staining (which allows separate on basis their proliferation rate) from colorectal cancer (CRC) xenografts subjected global gene expression pathway activation analyses. Factors expressed by were analyzed...
Circulating tumor cells (CTCs) are responsible for the metastatic dissemination of colorectal cancer (CRC) to liver, lungs and lymph nodes. CTCs rarity heterogeneity strongly limit elucidation their biological features, as well preclinical drug sensitivity studies aimed at metastasis prevention.
Objective To apply a machine learning analysis to clinical and presynaptic dopaminergic imaging data of patients with rapid eye movement (REM) sleep behavior disorder (RBD) predict the development Parkinson disease (PD) dementia Lewy bodies (DLB). Method s In this multicenter study International RBD group, 173 (mean age 70.5 ± 6.3 years, 70.5% males) polysomnography‐confirmed who eventually phenoconverted overt alpha‐synucleinopathy (RBD due synucleinopathy) were enrolled, underwent baseline...
Remyelination in the adult central nervous system has been demonstrated different experimental models of demyelinating diseases. However, there is no clear evidence that remyelination occurs multiple sclerosis, most diffuse disease. In this article, we explore possibility promoting myelination allergic encephalomyelitis, a widely used model by recruiting progenitors and channeling them into oligodendroglial lineage through administration thyroid hormone (T4). A large number proliferating...
Many studies indicate that oxygen free-radical formation after reoxygenation of liver may initiate the cascade hepatocellular injury. It has been demonstrated controlled ozone administration promote an oxidative preconditioning or adaptation to stress, preventing damage induced by reactive species and protecting against ischaemia-reperfusion (I/R) injury.In present study, effects (OzoneOP) on nitric oxide (NO) generation cellular redox balance have studied.Six groups rats were classified as...