A5046364084- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Microbial Natural Products and Biosynthesis
- Enzyme Structure and Function
- Nuclear Physics and Applications
- Mass Spectrometry Techniques and Applications
- Cancer therapeutics and mechanisms
- RNA Interference and Gene Delivery
- Crystallography and molecular interactions
- Genomics and Phylogenetic Studies
- Synthetic Organic Chemistry Methods
- Pharmacogenetics and Drug Metabolism
- Nanoparticle-Based Drug Delivery
- RNA and protein synthesis mechanisms
- Mycotoxins in Agriculture and Food
- Ginseng Biological Effects and Applications
- Asymmetric Synthesis and Catalysis
- Traditional and Medicinal Uses of Annonaceae
- Chemical Synthesis and Analysis
- Advanced Text Analysis Techniques
- Lysosomal Storage Disorders Research
- Viral gastroenteritis research and epidemiology
- Microfluidic and Bio-sensing Technologies
- Sentiment Analysis and Opinion Mining
- Genetics, Aging, and Longevity in Model Organisms
Elucid Bioimaging
2024
Lawrence Berkeley National Laboratory
2023-2024
University of California, Los Angeles
2019-2024
Harvey Mudd College
2016
The recent clinical and commercial success of lipid nanoparticles (LNPs) for nucleic acid delivery has incentivized the development new technologies to manufacture LNPs. As emerge, researchers must determine which assess how perform comparative evaluations. In this article, we use a quality-by-design approach systematically investigate mixer technology used form LNPs influences LNPstructure. Specifically, coaxial turbulent jet staggered herringbone microfluidic were compared via matched...
The lomaiviticins are dimeric genotoxic metabolites that contain unusual diazocyclopentadiene functional groups and 2–4 deoxyglycoside residues. Because only 6 of 19 carbon atoms in the monomeric aglycon unit proton-attached, their structure determination by NMR spectroscopic analysis is difficult. Prior elucidation efforts established two halves joined a single carbon–carbon bond appended to an oxidized cyclohexenone ring. This ring was believed comprise 4,5-dihydroxycyclohex-2-ene-1-one....
Here we apply microcrystal electron diffraction (MicroED) to the structural determination of transition-metal complexes. We find that simultaneous use 300 keV electrons, very low doses, and an ultrasensitive camera allows for collection data without cryogenic cooling stage. This technique reveals first crystal structures classic zirconocene hydride, colloquially known as "Schwartz's reagent", a novel Pd(II) complex not amenable solution-state NMR or X-ray crystallography, five other...
Medium-sized rings (8–11-membered cycles) are often more challenging to synthesize than smaller (5–7-membered due ring strain. Herein, we report a catalytic method for forming 8- and 9-membered that proceeds via the intramolecular Friedel–Crafts reactions of vinyl carbocation intermediates. These reactive species generated catalytically through ionization toluenesulfonates by Lewis acidic lithium cation–weakly coordinating anion salt.
The structural determination of natural products (NPs) can be arduous because sample heterogeneity. This often demands iterative purification processes and characterization complex molecules that may available only in miniscule quantities. Microcrystal electron diffraction (microED) has recently shown promise as a method to solve crystal structures NPs from nanogram quantities analyte. However, its implementation NP discovery remains hampered by throughput purity requirements, akin...
Lipid nanoparticles (LNPs) are being intensively researched and developed to leverage their ability safely effectively deliver therapeutics. To achieve optimal therapeutic delivery, a comprehensive understanding of the relationship between formulation, structure, efficacy is critical. However, vast chemical space involved in production LNPs resulting structural complexity make structure function challenging assess predict. New components formulation procedures, which provide new...
Few nucleoside-derived natural products have been identified from animals, despite the ubiquity of nucleosides in living organisms. Here, we use a combination synthesis and emerging electron microscopy technique microcrystal diffraction to determine structures several
4-Hydroxy-2-pyridone alkaloids have attracted attention for synthetic and biosynthetic studies due to their broad biological activities structural diversity. Here, we elucidated the pathway chemical logic of (−)-sambutoxin (1) biosynthesis. In particular, uncovered enzymatic origin tetrahydropyran moiety showed that p-hydroxyphenyl group is installed via a late-stage, P450-catalyzed oxidation phenylalanine-derived side chain rather than direct incorporation tyrosine.
Here we apply microcrystal electron diffraction (MicroED) to the structural determination of transition metal complexes. We find that simultaneous use 300 keV electrons, very low doses, and an ultra-sensitive camera allows for collection data without cryogenic cooling stage. This technique reveals first crystal structures classic zirconocene hydride, colloquially known as “Schwartz’s reagent”, a novel Pd(II) complex not amenable solution-state NMR or X-ray crystallography, five other...
The structural determination of natural products (NPs) can be arduous due to sample heterogeneity. This often demands itera-tive purification processes and characterization complex molecules that may only available in miniscule quantities. Microcrystal electron diffraction (microED) has recently shown promise as a method solve crystal structures NPs from nanogram quantities analyte. However, its implementation NP discovery remains hampered by throughput purity requirements akin traditional...
The lomaiviticins are dimeric genotoxic bacterial metabolites that contain unusual diazocyclopentadiene functional groups and 2–4 deoxyglycoside residues. Because only 6 of 19 carbon atoms in the monomeric aglycon unit proton-attached, their structure determination by NMR spectroscopic analysis is non-trivial. Prior elucidation efforts established two halves joined a single carbon–carbon bond appended to an oxidized cyclohexenone ring. This ring was believed comprise...
Here we apply microcrystal electron diffraction (MicroED) to the structural determination of transition metal complexes. We find that simultaneous use 300 keV electrons, very low doses, and an ultra-sensitive camera allows for collection data without cryogenic cooling stage. This technique reveals first crystal structures classic zirconocene hydride, colloquially known as “Schwartz’s reagent”, a novel Pd(II) complex not amenable solution-state NMR or X-ray crystallography, five other...
The lomaiviticins are dimeric genotoxic bacterial metabolites that contain unusual diazocyclopentadiene functional groups and 2–4 deoxyglycoside residues. Because only 6 of 19 carbon atoms in the monomeric aglycon unit proton-attached, their structure determination by NMR spectroscopic analysis is non-trivial. Prior elucidation efforts established two halves joined a single carbon–carbon bond appended to an oxidized cyclohexenone ring. This ring was believed comprise...