A5018817530- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Neuroendocrine regulation and behavior
- Neurobiology and Insect Physiology Research
- Spaceflight effects on biology
- Reproductive Biology and Fertility
- RNA Research and Splicing
- Genetic and Kidney Cyst Diseases
- Axon Guidance and Neuronal Signaling
- RNA and protein synthesis mechanisms
- Physiological and biochemical adaptations
- Insect Utilization and Effects
- Mitochondrial Function and Pathology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- Birth, Development, and Health
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Ecosystem dynamics and resilience
- RNA regulation and disease
- Gene expression and cancer classification
- Photosynthetic Processes and Mechanisms
- Psychological Treatments and Disorders
- Protein Degradation and Inhibitors
- Evolutionary Psychology and Human Behavior
University of Rochester
2016-2025
University of Rochester Medical Center
2004-2016
Institut thématique Génétique, génomique et bioinformatique
2014
Queens College, CUNY
2010
Institute of Developmental Physiology
2010
Albert Einstein College of Medicine
2000-2004
Howard Hughes Medical Institute
1994-1997
University of Pennsylvania
1993-1997
University of Tsukuba
1995
HnRNP proteins are abundant nucleoplasmic pre-mRNA-binding which have important roles in the biogenesis of mRNA. Although hnRNP been extensively characterized cultured cell lines, little is known about their expression animal tissues. Here, we undertaken a systematic survey major mouse tissue using specific monoclonal antibodies. Immunohistochemical staining demonstrated that C, L, and U were localized to nuclei all tissues examined. However, cytoplasmic A1, D, F/H, K was also detected...
As fundamental features of nearly all animal species, sexual dimorphisms and sex differences have particular relevance for the development function nervous system. The unique advantages nematode
The molecular mechanisms that control the timing of sexual differentiation in brain are poorly understood. We found sexually dimorphic postmitotic, sex-shared neurons nervous system Caenorhabditis elegans male is controlled by temporally regulated miRNA let-7 and its target lin-41, a translational regulator. lin-41 acts through lin-29a, an isoform conserved Zn finger transcription factor, expressed subset only male. Ectopic lin-29a sufficient to impose male-specific features at earlier...
The effect of the detailed connectivity a neural circuit on its function and resulting behavior organism is key question in many systems. Here, we study for nociception C. elegans, which composed same neurons two sexes that are wired differently. We show nociceptive sensory respond similarly sexes, yet animals display sexually dimorphic behaviors to aversive stimuli. To uncover role downstream network topology shaping behavior, learn simulate models replicate observed use them predict simple...
Abstract Recent studies of animal metabolism have revealed large numbers novel metabolites that are involved in all aspects organismal biology, but it is unclear to what extent metabolomes differ between sexes. Here, using untargeted comparative metabolomics for the analysis wildtype animals and sex determination mutants, we show C. elegans hermaphrodites males exhibit pervasive metabolomic differences. Several hundred small molecules produced exclusively or much larger amounts one sex,...
Although sexual dimorphism is ubiquitous in animals, the means by which sex determination mechanisms trigger specific modifications to shared structures not well understood. In C. elegans, tail tip morphology highly dimorphic: whereas hermaphrodites have a whip-like, tapered tip, male blunt-ended and round. Here we show that male-specific cell fusion retraction generate adult are controlled previously undescribed doublesex-related DM gene dmd-3, with secondary contribution from paralogous...
Sex differences in shared behaviors (for example, locomotion and feeding) are a nearly universal feature of animal biology. Though these may share underlying neural programs, their kinematics can exhibit robust between males females. The underpinnings poorly understood because the often-untested assumption that they determined by sex-specific body morphology. Here, we address this issue nematode Caenorhabditis elegans, which features two sexes with distinct morphologies but similar locomotor...
For sexually reproducing animals, selecting optimal mates is important for maximizing reproductive fitness. In the nematode C. elegans, populations reproduce largely by hermaphrodite self-fertilization, but cross-fertilization of hermaphrodites males also occurs. Males' ability to recognize involves several sensory cues, an integrated view ways use these cues in their native context assess characteristics potential has been elusive. Here, we examine mate-preference behavior elegans evoked...
ABSTRACT bHLH transcription factors function in neuronal development organisms as diverse worms and vertebrates. In the C. elegans male tail, a sublineage clonally gives rise to three cell types (two neurons structural cell) of each sensory ray. We show here that genes lin-32 hlh-2 are necessary for specification multiple fates within this sublineage, proper elaboration differentiated characteristics. Mutations lin-32, member atonal family, can cause failures at these steps, resulting...
Abstract Primary cilia have essential roles in transducing signals eukaryotes. At their core is the ciliary axoneme, a microtubule-based structure that defines cilium morphology and provides substrate for intraflagellar transport. However, extent to which axonemal microtubules are specialized sensory function unknown. In nematode Caenorhabditis elegans, primary present at dendritic ends of most neurons, where they provide environment transduction particular stimuli. Here, we find three...
Sexual maturation must occur on a controlled developmental schedule. In mammals, Makorin3 (MKRN3) and the miRNA regulators LIN28A/B are key of this process, but how they act is unclear. C. elegans, sexual nervous system includes functional remodeling postmitotic neurons onset adult-specific behaviors. Here, we find that lin-28–let-7 axis (the ‘heterochronic pathway’) determines timing these events. Upstream lin-28, Makorin lep-2 lncRNA lep-5 regulate cell-autonomously, indicating distributed...
Animals prioritize behaviors according to their physiological needs and reproductive goals, selecting a single behavioral strategy from repertoire of possible responses any given stimulus. Biological sex influences this decision-making process in significant ways, differentiating the animals choose when faced with stimuli ranging food conspecifics. We review here recent work invertebrate models, including C. elegans, Drosophila, variety insects, mollusks crustaceans, that has begun offer...