A5019835224- Nematode management and characterization studies
- Entomopathogenic Microorganisms in Pest Control
- Legume Nitrogen Fixing Symbiosis
- Geomechanics and Mining Engineering
- Insect Resistance and Genetics
- Insect behavior and control techniques
- Civil and Geotechnical Engineering Research
- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Rock Mechanics and Modeling
- Grouting, Rheology, and Soil Mechanics
- Plant Pathogens and Fungal Diseases
- Landslides and related hazards
- Insect and Pesticide Research
- Simulation and Modeling Applications
- Insect Pest Control Strategies
- Geological Modeling and Analysis
- Studies on Chitinases and Chitosanases
- Geotechnical and Geomechanical Engineering
- Geoscience and Mining Technology
- Plant-Microbe Interactions and Immunity
- Cryospheric studies and observations
- Climate change and permafrost
- Geotechnical Engineering and Analysis
- Cassava research and cyanide
Beijing Academy of Agricultural and Forestry Sciences
2022-2024
Institute of Plant Protection
2022-2024
Xi'an University of Science and Technology
2011-2023
Chinese Academy of Sciences
2007-2022
Huazhong Agricultural University
2019-2022
Lanzhou University
2022
Hunan Agricultural University
2007-2018
Huzhou University
2017
Hebei Academy of Agriculture and Forestry Sciences
2016
Shanghai Institutes for Biological Sciences
2013-2014
Parasitism genes expressed in the esophageal gland cells of phytonematodes encode secretions that control complex process plant parasitism. In soybean cyst nematode, Heterodera glycines, parasitome, i.e., secreted products parasitism genes, facilitate nematode migration roots and mediate modification root into elaborate feeding required to support growth development nematode. With very few exceptions, identities these are unknown, mechanisms parasitism, therefore, remain obscure. The most...
SUMMARY The Hg-SYV46 parasitism gene is expressed exclusively in the dorsal oesophageal gland cell of parasitic stages soybean cyst nematode, Heterodera glycines, and it encodes a secretory protein that contains C-terminal motif CLAVATA3/ESR-related (CLE) family Arabidopsis thaliana. In shoot floral meristems Arabidopsis, stem cells secret CLV3, founding member CLE family, activates CLV1/CLV2 receptor complex negatively regulates WUSCHEL expression to restrict size population. Mis-expression...
Identifying parasitism genes encoding proteins secreted from a nematode's esophageal gland cells and injected through its stylet into plant tissue is the key to understanding molecular basis of nematode plants. Meloidogyne incognita were cloned by microaspirating cytoplasm different parasitic stages provide mRNA create cell-specific cDNA library long-distance reverse-transcriptase polymerase chain reaction. Of 2,452 clones sequenced, deduced protein sequences 185 cDNAs had signal peptide for...
The common cutworm, Spodoptera litura (Fabricius), is a notorious and damaging insect pest of horticultural crops in China, the management which largely relies on chemical agents that are limited by development resistance target populations. As such, monitoring S. populations necessary part strategies insecticide resistance. In current work, we monitored to six insecticides field-collected sampled from eleven provinces across China 2021. results show developed significant against...
Cloning parasitism genes encoding secretory proteins expressed in the esophageal gland cells is key to understanding molecular basis of nematode plants. Suppression subtractive hybridization (SSH) with microaspirated contents from Heterodera glycines and intestinal region was used isolate preferentially parasitic stages. Twenty-three unique cDNA sequences a SSH library were identified hybridized genomic DNA H. Southern blots. Full-length cDNAs 21 clones obtained by screening gland-cell...
Abstract Background The gut bacteria of tephritid fruit flies play prominent roles in nutrition, reproduction, maintenance and ecological adaptations the host. Here, we adopted an approach based on direct observation symbiotic or axenic feeding dishes seeded with drops full diet (containing all amino acids) supplemented at similar concentrations to explore effects intestinal foraging decision fitness Bactrocera dorsalis . Results results show that probiotics elicit beneficial enhance female...
The evolution of feeding habits leads to speciation in insects. Bactrocera true fruit flies display diverse across species. We combine behavioral and functional genomic studies probe the divergence between specialist B. minax generalist dorsalis. find that both vision olfaction contribute their respective host preferences, with a dominant effect over short range. Correspondingly, location-related genes are significantly enriched phototransduction pathway, which long-wavelength rhodopsin...
Afidopyropen, a novel biopesticide, is derived from Aspergillus fumigatus, fungus, and shows promise as insecticidal agent for the management of whitefly pest Bemisia tabaci in horticultural economical crop production. In present work, we monitored susceptibilities B. to afidopyropen 18 field populations, sampled 9 provinces China, found that, comparison with susceptible strain (MED-S), most populations were highly susceptible, except Haidian population (HD) which exhibited an approximately...
SUMMARY The soybean cyst nematode, Heterodera glycines, produces beta-1,4-endoglucanases (cellulases) that are secreted during infection of soybean. gene structures three, hg-eng-4, hg-eng-5 and hg-eng-6, the six beta-1,4-endoglucanase genes, all family 5 glycosyl hydrolases previously identified from H. presented here. Furthermore, we present detailed expression analyses genes as well biochemical properties four glycines endoglucanase enzymes. Two endoglucanases, HG-ENG-5 HG-ENG-6, differed...
The potato rot nematode, Ditylenchus destructor, is a very destructive nematode pest on many agriculturally important crops worldwide, but the molecular characterization of its parasitism plant has been limited. effectors involved in for several sedentary endo-parasitic nematodes such as Heterodera glycines, Globodera rostochiensis and Meloidogyne incognita have identified extensively studied over past two decades. migratory parasitic different feeding behavior, life cycle host response....
Insect TRPV is one subfamily of transient receptor potential (TRP) channels, and it composed Inactive (Iav) Nanchung (Nan), the molecular targets afidopyropen in several sucking insect pests. In this study, we performed successive selection synergism tests based on previous work. The resistant strain HD-Afi Bemisia tabaci reached about 86-fold resistance to afidopyropen, only part resulted from detoxification cytochrome P450 monooxygenases. Then, cloned characterized Nan Iav B. (BtNan...
To expand the usage of endophytes in agriculture and forestry, insecticidal gene cry218 Bacillus thuringiensis was introduced into a poplar bacterial endophyte Burkholderia pyrrocinia JK-SH007. The cloned by polymerase chain reaction (PCR) inserted PHKT2 expression vector that By using sodium dodecyl sulphate polyacryl amide gel electrophoresis (SDS-PAGE) western blotting, we confirmed engineered successfully constructed, it harboured function after bioassay planta. toxicity expressed...
Alpine vegetation on the Southwestern Tibetan Plateau (SWTP) is sensitive and vulnerable to climate change human activities. Climate warming actions (mainly ecological restoration, social-economic development, grazing) have already caused degradation of alpine grasslands (TP) some extent. However, it remains unclear how activities regulated variation under restoration since 2000. This study used normalized difference index (NDVI) social statistic data explore spatiotemporal changes...
The soybean cyst nematode, Heterodera glycines, is an important pest of soybeans. Although resistance available against this selection for virulent races can occur, allowing the nematode to overcome cultivars. There are abundant field populations, however, little known about their genetic diversity. In order elucidate differences between races, we investigated transcriptional diversity within race 3 and 4 inbred lines during compatible interactions with host Zhonghuang 13. Six different...
A new anthracnose disease on chili pepper (cayenne cv. Hongxiu 2003, fruiting type pepper) was found in Zhijiang County, Hunan, China 2009. The observed only the fruits. Lesions were generally elongated, which dark acervuli arranged concentrically. Later, cracking of older lesions observed. With a microscope, fungal conidia to be 15.8 × 4.1 μm, fusiform or oval with one end acute, and single celled two seven oil globules. No setae acervuli. Eight isolates (HNZJ001-HNZJ008) showed no...
Pair-rule genes were identified and named for their role in segmentation animal embryos. Paired, a homolog of mammalian PAX3, acts as one several pair-rule is key defining the boundaries future parasegments segments during insect embryogenesis.We cloned paired gene from oriental fruit fly, Bactrocera dorsalis, then applied CRISPR/Cas9-mediated genome editing to investigate its physiological function embryonic stage this pest. We transcript B. which encodes protein containing Paired Box...