The common cutworm, Spodoptera litura (Fabricius), is a notorious and damaging insect pest of horticultural crops in China, the management which largely relies on chemical agents that are limited by development resistance target populations. As such, monitoring S. populations necessary part strategies insecticide resistance. In current work, we monitored to six insecticides field-collected sampled from eleven provinces across China 2021. results show developed significant against...

Odorant binding proteins (OBPs) and chemosensory (CSPs) of arthropods are thought to be involved in chemical recognition which regulates pivotal behaviors including host choice, copulation reproduction. In insects, OBPs CSPs located mainly the antenna but they have not been systematically characterized yet Bemisia tabaci is a cryptic species complex could damage more than 600 plant species. this study, among 106,893 transcripts head assembly, 8 13 were identified B. MED based on...

Aphids have been mainly controlled by traditional chemical insecticides, resulting in unamiable risk to the environment over last decades. Push-pull strategy is regarded as a promising eco-friendly approach for aphid management through repelling away and attracting their natural enemy. Methyl salicylate (MeSA), one of typical HIPVs (herbivore-induced plant volatiles), can repel aphids attract ladybugs. Our previous studies discovered new lead compound 3e, salicylate-substituted carboxyl...

Abstract Spodoptera litura is a notoriously destructive pest, which infests horticultural plants and economically important crops worldwide. Several generations of diamide insecticides have been used to combat S. throughout the world, causing various levels resistance this class among wild populations. In work, we monitored five 10 populations sampled from eastern China in 2022. comparison with Lab‐S susceptible strain, tested displayed high susceptibility three (cyantraniliprole,...

Harmonia axyridis is an important natural enemy that consumes many agricultural and forestry pests. It relies on a sensitive olfactory system to find prey mates. Odorant-binding proteins (OBPs) as the first-step of recognizing volatiles, transport odors through sensillum lymph odorant receptors (ORs). However, little known about molecular mechanisms H. olfaction. In this study, four antenna specific OBP genes, HaxyOBP3 , 5 12 15 were bacterially expressed binding features recombinant 40...

Spodoptera litura is a damaging and notorious insect pest of agricultural crops that has developed resistance to various insecticides. Broflanilide novel pesticide with unique mode action displays high efficiency against lepidopterous larvae. We here determined the baseline susceptibility laboratory strain S. broflanilide 10 other popular Furthermore, we measured cross-resistance using three common insecticides in 11 field-collected populations. caused highest toxicity among all tested...

Bemisia tabaci is a threat to agriculture worldwide because of its potential cause devastating damage crops. Chlorogenic acid bioactive pesticidal phytochemical agent against various insect pests. We here determined the susceptibility laboratory strain B. chlorogenic and other popular insecticides, several field-collected populations acid. Also, cross-resistance four common insecticides was measured. had highest toxicity all tested were susceptible acid, little detected between insecticides....

: The whitefly, Bemisia tabaci, is an important invasive economic pest of agricultural crops worldwide. β-ionone has a significant oviposition repellent effect against B. but the olfactory molecular mechanism this insect for recognizing unclear. To clarify binding properties odorant-binding proteins (OBPs) with β-ionone, we performed gene cloning, evolution analysis, bacterial expression, fluorescence competitive assay, and docking to study function OBP1 OBP4 on β-ionone. results showed that...

Afidopyropen, a novel biopesticide, is derived from Aspergillus fumigatus, fungus, and shows promise as insecticidal agent for the management of whitefly pest Bemisia tabaci in horticultural economical crop production. In present work, we monitored susceptibilities B. to afidopyropen 18 field populations, sampled 9 provinces China, found that, comparison with susceptible strain (MED-S), most populations were highly susceptible, except Haidian population (HD) which exhibited an approximately...

The sycamore lace bug, Corythucha ciliata, is a pest of trees. In China, it found in the most northern border where has been known to become dormant during harsh winters. But molecular and metabolic basis for dormancy this insect still unknown. study, we analyzed transcript metabolite profiles bug identify key genes metabolites that are significantly regulated adult females males. total, 149 differentially expressed (DEGs) were up-regulated 337 DEGs down-regulated adults (both males). We...

Harmonia axyridis (Coleoptera: Coccinellidae) is a polyphagous insect that an important biological agent used to control agricultural and forestry pests. The role of functional genes in H. based on quantitative real-time PCR (qRT-PCR) increasingly well understood investigate biology, physiology, feeding behavior the physiological processes. Quantitative powerful reliable technique quantify gene expression. Using qRT-PCR, expression levels target are determined internal reference genes;...

Abstract BACKGROUND Chemosensory proteins (CSPs) are thought to play essential roles in insect chemical communication, but their exact physiological functions remain unclear. RESULTS In this study, we investigated the of CSP2 gene whitefly Bemisia tabaci using protein expression and binding affinity spectrum different types odor molecules. Moreover, evolutionary characteristics were studied. The data obtained assay showed that can bind a broad range plant volatiles including homoterpene ( E...

The cultivated tomato Solanum lycopersicum suffered a severe attack by the whitefly Bemisia tabaci (Gennadius), causing damage to leaves feeding as well transmitting yellow leaf curl virus (TYLCV), while wild S. habrochaites is considerably less appealing this insect species. It reported that B. shows innate avoidance R-curcumene, which produced naturally habrochaites. However, mechanisms involved in behavior of response chiral compound are still unclear yet. In study, functional and binding...

Bemisia tabaci , the tobacco whitefly, is one of most notorious agricultural sucking insect pests that severely damage a series crops worldwide. Throughout China, B. threatens production with increasing cases resistance to commonly used insecticides, prompting widespread use cyantraniliprole as an alternative control hemipteran pests. Here, we found overexpression CYP4G68 gene conferring using quantitative real-time PCR (qPCR) and RNA interference (RNAi) in lab-selected resistant strain...