Geodermatophilaceae (order Geodermatophilales, class Actinobacteria) form a comparatively isolated family within the phylum Actinobacteria and harbour many strains adapted to extreme ecological niches tolerant against reactive oxygen species. Clarifying evolutionary history of was so far mainly hampered by insufficient resolution main phylogenetic marker in use, 16S rRNA gene. In conjunction with taxonomic characterisation motile aerobic strain, designated YIM M13156T phylogenetically...

Genome-scale phylogenetic analysis based on core gene sets is routinely used in microbiological research. However, the techniques are still not approachable for individuals with little bioinformatics experience. Here, we present EasyCGTree, a user-friendly and cross-platform pipeline to reconstruct genome-scale maximum-likehood (ML) tree using supermatrix (SM) supertree (ST) approaches.EasyCGTree was implemented Perl programming languages built collection of published reputable programs. All...

The pore-fracture structure of deep coal reservoirs is highly important for evaluating, exploring, and developing coalbed methane (CBM) resources. This study considers three samples from the DJ57 well in Benxi Formation Daning–Jixian block on eastern margin Ordos Basin as research object. Based quality parameters samples, field emission scanning electron microscopy (FE-SEM), gas adsorption experiments, high-pressure mercury intrusion porosimetry (MIP), microcomputed tomography (micro-CT)...

The wide usage of antibiotics contributes to the increase in prevalence antibiotic-resistant Salmonella. Plasmids play a critical role horizontal transfer antibiotic resistance markers This study aimed screen and characterize plasmid profiles responsible for Salmonella ultimately clarify molecular mechanism transferable plasmid-mediated resistance. A total 226 isolates were examined antimicrobial susceptibility by disk diffusion method. Thirty-two (14.2%) resistant at least one antibiotic....

Aim: To investigate whether the Staphylococcus argenteus is present in Eastern China and to verify utility of a new screening process. Materials & methods: Phenotype observation, PCR assay targeting hypothetical nonribosomal peptide synthetase (NRPS) gene, phylogenetic analysis rpoB multilocus sequence typing were used screen identify strains S. from 839 presumptive aureus isolates. Results: Eighty-nine (89/839, 10.6%) isolates produced white colonies on tryptone soya agar plates. Of...

The families Paracoccaceae and Roseobacteraceae comprise metabolically, phenotypically genotypically diverse members, their descriptions rely heavily on 16S rRNA gene analysis. Hundreds of genera have been reported within the two misclassifications a reoccurring problem, even when taxonomies established based genome-scale phylogenetic reconstructions. In this study, we conducted comprehensive phylotaxonomic assessment four ubiquitous sets, bac120 (120 genes in Bacteria), rhodo268 (268 ‘...

Staphylococcus argenteus and S. schweitzeri, were recently proposed as novel species within aureus complex (SAC). has been reported in many countries can threaten human health. schweitzeri not associated with infections, but isolated from non-human primates. Questions regarding the evolution of pathogenicity these two will remain elusive until an exploratory evolutionary framework is established.We present genomic comparison analysis among members SAC based on a pan-genome definition, which...

On the basis of comprehensive analysis drilling, gas testing, laboratory and testing data, characteristics genesis tight carbonate reservoirs in Ma51+2 Member Ordovician Majiagou Formation, eastern Yi-Shan slope, Ordos Basin were examined, potential natural exploration development analyzed. The reservoir is defined as with a porosity less than 2% permeability 0.1×10−3 μm2. are dominantly gypsum mud dolomite, muddy dolomite Karst-breccia has strong heterogeneity, pore types being mainly...

Staphyloxanthin (STX), a golden carotenoid pigment produced by Staphylococcus aureus, is suggested to act as an important virulence factor due its antioxidant properties. Restraining biosynthesis of STX was considered indicator decline in pigmented S. aureus isolates. However, it not clear whether natural non-pigmented isolates have less than ones. In this study, aimed compare the and clarify genetic virulent differences between two groups. Here, 132 were divided into phenotype groups...

Excessive cadmium (Cd) accumulation in soil can adversely affect plants, animals, microbes, and humans; therefore, novel uncharacterized Cd-resistant plant-growth-promoting rhizobacteria (PGPR) are required to address this issue. In the paper, 13 bacteria were screened, their partial 16S rRNA sequences determined, isolates, respectively, clustered into Curtobacterium (7), Chryseobacterium (4), Cupriavidus (1), Sphingomonas (1). Evaluation of PGP traits, including indole-3-acetic acid (IAA)...

ABSTRACT This study analyses various factors that affect the threshold voltage of MOSFETs at deep cryogenic temperatures, including band‐tail state, field‐assisted ionization, and interface traps. Based on analysis, a new model is developed for Si‐based covering wide temperature range from 10 to 300 K. The validity confirmed through experiments bulk silicon MOSFETs, FDSOI n‐FinFET.

Two actinomycete strains isolated from marine sediment samples, designated YIM M11335 T (from the Indian Ocean) and 12A09 South China Sea), were obtained examined by a polyphasic approach. The two Gram-staining-positive, aerobic produced branched substrate mycelia aerial hyphae that not fragmented, no diffusible pigment was on media tested. At maturity, spore chains single spores formed mycelium, respectively. Whole-organism hydrolysates of both contained meso -diaminopimelic acid diagnostic...

Internalins are surface proteins that utilized by Listeria monocytogenes to facilitate its invasion into human intestinal epithelial cells. The expression of a full-length InlA is one essential virulence factors for L. cross the barrier in order invade In this study, gene sequences inlA 120 isolates from food (n = 107) and humans 13) were analyzed. Premature stop codon (PMSC) mutations identified 51 (50 1 human). Six mutation types PMSCs identified. Among with inlA, there 44 serogroup 1/2c,...

To conquer the trade-off dilemma of nanofiltration membranes for both high permeability water and rejection pollutants has long been highly concerned. In this work, TiO2 Bi2S3 nanoparticles were evenly dispersed successively into interlayers Ti3C2Tx nanosheets, then filtered onto cellulose acetate support membranes. The as-obtained composite exhibited a maximum flux 374.4 L m−2 h−1 bar−1, ratio RhB, GR, MB BSA at 97.8–100%, apparently higher than those related in literature. Such separation...

A novel endophytic actinobacterium, designated strain YIM 67072 T , was isolated from healthy roots of Dysophylla stellata (Lour.) Benth. Cells this aerobic, cream–yellow-coloured occurred singly, in pairs or tetrads, were Gram-stain-positive and ovoid- to spherical-shaped. Strain grew at 4–45 °C, pH 5.0–10.0 the presence 0–7 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that belonged genus Rothia . The isolate contained MK-7 as major component quinone system....