A5060774903- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Cancer therapeutics and mechanisms
- DNA Repair Mechanisms
- RNA modifications and cancer
- Gene expression and cancer classification
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Neutropenia and Cancer Infections
- CRISPR and Genetic Engineering
- Prostate Cancer Treatment and Research
- Breast Cancer Treatment Studies
- Cancer-related Molecular Pathways
- Esophageal Cancer Research and Treatment
- Chromosomal and Genetic Variations
- Genomic variations and chromosomal abnormalities
- Photosynthetic Processes and Mechanisms
- Berberine and alkaloids research
- Histone Deacetylase Inhibitors Research
- HER2/EGFR in Cancer Research
- Lung Cancer Research Studies
- Cytomegalovirus and herpesvirus research
- Cancer Genomics and Diagnostics
- Carcinogens and Genotoxicity Assessment
Texas Southern University
2014-2024
Genesis Foundation
2007-2023
National Cancer Institute
1990-2012
National Institutes of Health
2004-2012
Center for Cancer Research
2003-2012
The University of Texas MD Anderson Cancer Center
2009-2010
Shanghai Institute of Materia Medica
2007
Science Applications International Corporation (United States)
2007
Population Council
2004
Molecular Oncology (United States)
2001
We immunohistochemically examined 12 core proteins involved in the chromatin remodeling machinery using a tissue microarray composed of 150 lung adenocarcinoma (AD) and squamous cell carcinoma (SCC) cases. Most showed nuclear staining, whereas some also cytoplasmic or membranous staining. When expression patterns all tested antigens were considered, with staining clustered into two major groups. Nuclear signals BRM, Ini-1, retinoblastoma, mSin3A, HDAC1, HAT1 together, BRG1, BAF155, HDAC2,...
We have developed a branched DNA in situ hybridization (bDNA ISH) method for detection of human papillomavirus (HPV) whole cells. Using cervical cancer cell lines with known copies HPV DNA, we show that the bDNA ISH is highly sensitive, detecting as few one or two per cell. By modifying sample pretreatment, viral mRNA sequences can be detected using same set oligonucleotide probes. In experiments performed on mixed populations cells, specific and distinguish cells HPV-16 from HPV-18 DNA....
Glucocorticoids, major end effectors of the stress response, play an essential role in homeostasis central nervous system and influence diverse functions neuronal cells. We found that cyclin-dependent kinase 5 (CDK5), which plays important roles morphogenesis whose aberrant activation is associated with development neurodegenerative disorders, interacted ligand-binding domain glucocorticoid receptor (GR) through its activator p35 or active proteolytic fragment p25. CDK5 phosphorylated GR at...
A group xeroderma pigmentosum revertant with normal sensitivity was created by chemical mutagenesis. It repaired (6-4) photoproducts normally but not pyrimidine dimers and had near levels of repair replication, sister chromatid exchange, mutagenesis from UV light. The rate UV-induced mutation in a shuttle vector, however, as high the parental cell line.
Abstract The biological functions of nuclear topoisomerase I (Top1) have been difficult to study because knocking out TOP1 is lethal in metazoans. To reveal the human Top1, we generated stable Top1 small interfering RNA (siRNA) cell lines from colon and breast carcinomas (HCT116-siTop1 MCF-7-siTop1, respectively). In those clones, reduced ∼5-fold Top2α compensates for deficiency. A prominent feature siTop1 cells genomic instability, with chromosomal aberrations histone γ-H2AX foci associated...
We used hierarchical clustering to examine gene expression profiles generated by serial analysis of (SAGE) in a total nine normal lung epithelial cells and non-small cell cancers. Separation tumor, as well histopathological subtypes, was evident using the 3,921 most abundant transcript tags. This distinction remained when only 115 highly differentially expressed tags were used. Furthermore, these clustered into groups suggestive unique biological pathological features different tissues...
Previous studies have implicated the rasHa oncogene in initiation of skin carcinogenesis and fos malignant progression premalignant cell lines. To determine if these two oncogenes are sufficient to convert normal keratinocytes cancer cells, freshly isolated mouse were coinfected with replication-defective (psi-2) v-rasHa v-fos viruses culture. When tested nude mice within several days infection, v-fos/v-rasHa-coinfected produced squamous carcinomas. Introduction alone resulted or...
Profiling the dynamic interaction of p300 with proximal promoters human T cells identified a class genes that rapidly coassemble and RNA polymerase II (pol II) following mitogen stimulation. Several these targets are immediate early genes, including FOS, implicating prominent role for in control primary genetic responses. The recruitment pol transitions to assembly several elongation factors, positive transcriptional factor (P-TEFb), bromodomain-containing protein (BRD4), elongin-like eleven...
Abstract— The relative biological importance of(5–5,6–6) cyclobutane and(6–4) pyrimidine‐pyrimidone dimers in mammalian cells has been determined a xeroderma pigmentosum (XP) revertant that repairs only the(6–4) photoproduct. Surprisingly, the majority of effects UV light, including cell killing, sister chromatid exchange, mutagenesis, and inhibition recovery DNA RNA synthesis, appear to be due photoproduct not dimer. Although XP its own genomic DNA, it fails repair damage shuttle vectors,...
Characterization of gene expression profiles in tumor cells and the microenvironment is an important step understanding neoplastic progression. To date, there are limited data available on changes that occur tumor-associated stroma as either a cause or consequence cancer. In present study, we employed 54,000 target oligonucleotide microarray to compare 4 major components microenvironment: epithelium, stroma, normal stroma. Cells from 5 human, whole-mount prostatectomy specimens were...
HIN-1 (high in normal-1) is a candidate tumor suppressor identified as gene silenced by methylation the majority of breast carcinomas. highly expressed mammary gland, trachea, lung, prostate, pancreas, and salivary its expression primarily restricted to bronchial epithelial cells. In this report, we show that, correlating with secretory nature HIN-1, high levels protein are detected lavage, saliva, plasma, serum. To determine if, similar carcinomas, also tumors originating from other organs...
The MYBL1 gene is a strong transcriptional activator involved in events associated with cancer progression. Previous data show overexpressed triple-negative breast (TNBC). There are two parts to this study related further characterizing the gene. We start by reference sequence variants and isoforms. results of will help future experiments event there need characterize functional isoforms In part two, we identify validate expression gene-related alterations MYBL1, VCIP1, MYC BOP1 genes TNBC...
Esophageal squamous cell carcinoma (ESCC), the predominant histological subtype of esophageal cancer, is characterized by high mortality. Previous work identified important mRNA expression differences between normal and tumor cells; however, to date there are limited ex vivo studies examining changes occurring during differentiation versus those associated with tumorigenesis. In this study, we used a unique tissue microdissection strategy microarrays measure gene profiles tumorigenesis in...
Recent studies have focused on transcriptional regulation and gene expression profiling of human embryonic stem cells (hESCs). However, little information is available regarding the relationship between RNA regulation, which critical in complete understanding pluripotency differentiation hESCs. In current study, we determined three different hESC lines compared to Human universal reference (HuU-RNA) using a full genome microarray, our results target genes previously identified ChIP-on-chip...
When triple negative breast cancer (TNBC) are analyzed by gene expression profiling different subclasses identified, at least one characterized genes related to immune signaling mechanisms supporting the role of these in cancers. In an earlier study we observed differences TNBC cell lines with respect their cytokine IL32. Our analyses showed that certain expressed higher levels compared others. Because heterogeneous and immune-related appear play a pivotal cancers, chose examine...
Abstract Motivation: For Affymetrix microarray platforms, gene expression is determined by computing the difference in signal intensities between perfect match (PM) and mismatch (MM) probesets. Although use of PM not controversial, MM probesets have been associated with variance ultimately inaccurate calls. A principal focus this study was to investigate nature demonstrate its contribution experimental results. Results: While most were likely random noise, a subset ∼20% (99 485) probes...
Abstract TDE2 , a gene with sequence similarity to the mouse testicular tumor‐differentially‐expressed ( Tde1 / MUSTETU ) gene, was identified by serial analysis of expression (SAGE) in nonsmall cell lung cancers (NSCLC). Here we characterized and determined its transcript levels panel tumors, adjacent nonmalignant tissues variety normal human tissues. In addition, show that is potential transmembrane protein 11 putative helices. Using real‐time quantitative PCR, showed were higher NSCLC...
We have demonstrated in vitro transcription (IVT) of cDNA sequences from purified Jurkat T-cell mRNA immobilized on microfluidic packed beds down to single-cell quantities. The microfluidically amplified antisense-RNA (aRNA) was nearly identical length and quantity compared with benchtop reactions using the same starting sample Microarrays were used characterize number population genes each sample, allowing comparison processes. For both assays, we measured expression approximately 4000 9000...
A group xeroderma pigmentosum revertant with normal sensitivity was created by chemical mutagenesis. It repaired (6-4) photoproducts normally but not pyrimidine dimers and had near levels of repair replication, sister chromatid exchange, mutagenesis from UV light. The rate UV-induced mutation in a shuttle vector, however, as high the parental cell line.
Triple negative breast cancer (TNBC) and often basal-like cancers are defined as for estrogen receptor, progesterone receptor Her2 gene expression. Over the past few years an incredible amount of data has been generated defining molecular characteristics both cancers. The aim these studies is to better understand identify genes pathways that might be useful targeted therapies. In attempt contribute understanding basal-like/TNBC, we examined Gene Expression Omnibus (GEO) public datasets in...