A5047586630- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Algal biology and biofuel production
- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- Photonic and Optical Devices
- Micro and Nano Robotics
- Conservation, Biodiversity, and Resource Management
- Semiconductor Lasers and Optical Devices
- Fire effects on ecosystems
- Hydrology and Watershed Management Studies
- Photoreceptor and optogenetics research
- Advanced Fiber Optic Sensors
- Soil erosion and sediment transport
- CRISPR and Genetic Engineering
- Fish biology, ecology, and behavior
- Land Use and Ecosystem Services
- Plant Water Relations and Carbon Dynamics
- Photorefractive and Nonlinear Optics
- Lipid metabolism and biosynthesis
- Ecology and Vegetation Dynamics Studies
- Advanced Fiber Laser Technologies
- Pancreatic function and diabetes
- Viral Infectious Diseases and Gene Expression in Insects
- Geography and Environmental Studies
University of Minnesota
2010-2024
Twin Cities Orthopedics
2024
Woodwell Climate Research Center
2005-2021
University of Florida
2017
Southwest Bahia State University
2015
Universidade de Brasília
2011-2015
Instituto de Pesquisa Ambiental da Amazônia
1999-2015
Universidade de São Paulo
2015
Tall Timbers Research Station and Land Conservancy
2011
University of California System
2011
Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It model system for studying chloroplast-based photosynthesis, as well the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited common ancestor animals, but lost in plants. We sequenced ∼120-megabase nuclear genome performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated...
Abstract: Conservation scientists generally agree that many types of protected areas will be needed to protect tropical forests. But little is known the comparative performance inhabited and uninhabited reserves in slowing most extreme form forest disturbance: conversion agriculture. We used satellite‐based maps land cover fire occurrence Brazilian Amazon compare large (>10,000 ha) (parks) (indigenous lands, extractive reserves, national forests) reserves. Reserves significantly reduced...
The incidence and importance of fire in the Amazon have increased substantially during past decade, but effects this disturbance force are still poorly understood. forest dynamics two regions eastern were studied. Accidental fires affected nearly 50 percent remaining forests caused more deforestation than has intentional clearing recent years. Forest create positive feedbacks future susceptibility, fuel loading, intensity. Unless current land use practices changed, potential to transform...
Abstract The hydrological connectivity of freshwater ecosystems in the Amazon basin makes them highly sensitive to a broad range anthropogenic activities occurring aquatic and terrestrial systems at local distant locations. are suffering escalating impacts caused by expansions deforestation, pollution, construction dams waterways, overharvesting animal plant species. natural functions these changing, their capacity provide historically important goods services is declining. Existing...
Abstract Severe drought in moist tropical forests provokes large carbon emissions by increasing forest flammability and tree mortality, suppressing growth. The frequency severity of the tropics may increase through stronger El Niño Southern Oscillation (ENSO) episodes, global warming, rainfall inhibition land use change. However, little is known about spatial temporal patterns forests, complex relationships between fire regimes, productivity. We present a simple geographic information system...
Moist tropical forests in Amazonia and elsewhere are subjected to increasingly severe drought episodes through the El Niño–Southern Oscillation (ENSO) possibly deforestation‐driven reductions rainfall. The effects of this trend on forest canopy dynamics, emissions greenhouse gases, other ecological functions potentially large but poorly understood. We established a throughfall exclusion experiment an east‐central Amazon (Tapajós National Forest, Brazil) help understand these effects. After...
We have developed a nuclear transformation system for Chlamydomonas reinhardtii, using micro-projectile bombardment to introduce the gene encoding nitrate reductase into nit1 mutant strain which lacks activity. By either supercoiled or linear plasmid DNA, transformants were recovered consistently at low efficiency, on order of 15 per microgram DNA. In all cases transforming DNA was integrated genome, usually in multiple copies. Most introduced copies genetically linked each other, and they...
The green alga Chlamydomonas reinhardtii is a leading unicellular model for dissecting biological processes in photosynthetic eukaryotes. However, its usefulness has been limited by difficulties obtaining mutants specific genes of interest. To allow generation large numbers mapped mutants, we developed high-throughput methods that (1) enable easy maintenance tens thousands strains propagation on agar media and cryogenic storage, (2) identify mutagenic insertion sites physical coordinates...
Synthesis of new proteins is required to regenerate full length Chlamydomonas flagella after deflagellation. Using gametes, which have a low basal level protein synthesis, it has been possible label and detect the synthesis many flagellar in whole cells. The deflagellation-induced tubulins, dyneins, membrane protein, at least 20 other co-migrate with isolated axonemes, can be detected gamete cytoplasm, times initiation termination for each studied. nature signal that stimulates cell initiate...
Abstract Chlamydomonas is a popular genetic model system for studying many cellular processes. In this report, we describe new approach to isolate genes using the cloned nitrate reductase gene (NIT1) as an insertional mutagen. A linearized plasmid containing NIT1 was introduced into nit1 mutant cells by glass-bead transformation. Of 3000 Nit+ transformants examined, 74 showed motility defects of wide range phenotypes, suggesting that DNA transformation effective method mutagenizing cells....
The nitrate reductase structural gene of Chlamydomonas reinhardtii has been isolated from a genomic library by using cDNA probe barley. Restriction fragment length polymorphism analyses mapped the clone (B6a) to locus nit-1. Overlapping inserts cover region genome about 24 kilobases containing entire gene, which spans approximately 5-8 kilobases. Sequence analysis DNA fragments B6a demonstrated high degree sequence similarity at amino acid level with regions corresponding portions heme and...
Abstract Anthropogenic understory fires affect large areas of tropical forest, particularly during severe droughts. Yet, the mechanisms that control forests' susceptibility to fire remain ambiguous. We tested widely accepted hypothesis Amazon forest increase further burning by conducting a 150 ha experiment in closed‐canopy near southeastern forest–savanna boundary. Forest flammability and its possible determinants were measured adjacent 50 plots burned annually for 3 consecutive years (B3),...
Abstract Genetic evidence suggests that the NIT2 gene of Chlamydomonas reinhardtii encodes a positive regulator nitrate-assimilation pathway. To learn more about function product, we isolated using transposon-tagging strategy. A nit2 mutation caused by insertion transposon was identified testing spontaneous mutants for presence new copies Gulliver or TOC1, transposable elements have been in Chlamydomonas. In 2 14 different were analyzed, element found to be genetically and phenotypically...
The interaction between droughts and land-use fires threaten the carbon stocks, climate regulatory functions, biodiversity of Amazon forests, particularly in southeast, where deforestation ignitions are high. Repeated, severe, or combined result tropical forest degradation via nonlinear dynamics may lead to an alternate vegetation state. Here, we discuss major insights from longest (more than 10 years) largest (150-hectare) experimental burn forests. Despite initial resistance low-intensity...