A5008005360- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Carbohydrate Chemistry and Synthesis
- Ubiquitin and proteasome pathways
- Toxin Mechanisms and Immunotoxins
- PARP inhibition in cancer therapy
- RNA modifications and cancer
- RNA Research and Splicing
- Histone Deacetylase Inhibitors Research
- Transgenic Plants and Applications
- Microtubule and mitosis dynamics
- Peptidase Inhibition and Analysis
- Marine Sponges and Natural Products
- 14-3-3 protein interactions
- Microbial Natural Products and Biosynthesis
- Adenosine and Purinergic Signaling
- Plant pathogens and resistance mechanisms
- Protein Tyrosine Phosphatases
- Bone Metabolism and Diseases
- Banana Cultivation and Research
- Calcium signaling and nucleotide metabolism
- Electrostatic Discharge in Electronics
- Zebrafish Biomedical Research Applications
- Protein Degradation and Inhibitors
- Marine Toxins and Detection Methods
Nagahama Institute of Bio-Science and Technology
2010-2024
RIKEN Advanced Science Institute
2008
Japan Science and Technology Agency
2008
Johns Hopkins University
2002-2003
Johns Hopkins Medicine
2002-2003
Mie University
1993-1996
Liberal Arts University
1991
Shiga University
1991
Previously, we reported that c-Myc is glycosylated by <i>O</i>-linked <i>N</i>-acetylglucosamine at Thr-58, a known phosphorylation site and mutational hot spot in lymphomas. In this paper, describe the production characterization of two Thr-58 site-specific antibodies use them to examine modification living cells. One antibody specifically reacts with Thr-58-glycosylated form c-Myc, other only unmodified c-Myc. Using these together commercial anti-Thr-58-phosphorylated antibody,...
Mitochondria perform multiple functions critical to the maintenance of cellular homeostasis. Here we report that downregulation histone deacetylase 6 (HDAC6) causes a reduction in net activity mitochondrial enzymes, including respiratory complex II and citrate synthase. HDAC6 ubiquitin‐binding activities were both required for recovery reduced metabolic due loss HDAC6. Hsp90, substrate HDAC6, localizes mitochondria partly mediates regulation by Our finding suggests regulates metabolism might...
Acetylation of α-tubulin on Lys(40) is thought to be a modification that regulates the dynamic instability microtubules, but little known about occurrence acetylation in plants. Here we report growth stage-dependent change levels and organ distribution acetylated Arabidopsis thaliana Widespread leaves 15 species (20 cultivars) angiosperms was also confirmed. Our data indicate widespread many angiosperms, can differ, sometimes considerably, among different organs developmental stages.
A new C30 linear polyacetylene compound designated petroacetylene (1) has been isolated from the marine sponge Petrosia solida Hoshino 1981, collected off coast of Amami-Oshima, Kagoshima Prefecture, Japan. The structure was elucidated on basis spectroscopic data and chemical means. Petroacetylene inhibited blastulation starfish embryos at a concentration 3.1 μg mL− 1 or greater.
Protein targets of polyADP-ribosylation undergo covalent modification with high-molecular-weight, branched poly(ADP-ribose) (PAR) lengths up to 200 or more ADP-ribose residues derived from NAD+. PAR polymerase 1 (PARP1) is the most abundant and well-characterized enzyme involved in biosynthesis. Extensive studies have been carried out determine how (PARylation) regulates cell proliferation during cycle, conflicting conclusions. Since significant activation PARP1 occurs lysis vitro, we...
Abstract Of the FET (fused in sarcoma [FUS]/Ewing protein [EWS]/TATA binding protein-associated factor 15 [TAF15]) family of heterogeneous nuclear ribonucleoprotein particle proteins, FUS and TAF15 are consistently EWS variably found inclusion bodies neurodegenerative diseases such as frontotemporal lobar degeneration associated with FUS. It is speculated that dysregulation proteins at post-translational level involved their cytoplasmic deposition. Here, O-linked β-N-acetylglucosamine...
Abstract Cholangiocarcinoma is one of the most serious diseases in northeast T hailand, where its incidence reported to be highest world. We tried develop a new method detect cholangiocarcinoma early stages using serum proteins. found that after fluorescent labeling sugar moiety proteins, peak was identified, which might promising marker for cholangiocarcinoma.
Recently, we confirmed the widespread occurrence of α-tubulin acetylation on Lys(40) in angiosperms. In present study, found that is regulated a growth stage- and organ development-dependent manner rapid cycling Brassica rapa, also known as Fast Plants. Organ distribution analysis showed proportion acetylated high cotyledons young plants true leaves flowers mature plants. A correlation between increase levels maturation was observed. leaves, an uneven pattern, cells region leaf margins...
A new brominated C(17) acetylenic acid (1) designated as bromotheoynic has been isolated from the marine sponge Theonella swinhoei, collected off coast of Tanegashima, Kagoshima Prefecture, Japan. The structure was determined on basis analysis its extensive 2D NMR spectroscopic data well HRMS. Bromotheoynic inhibited maturation starfish oocytes and cell division fertilised eggs. also proliferation human leukaemia U937 HL60 cells, lung cancer A549 H1299 embryonic kidney 293 (HEK293) cells.
Although Ewing sarcoma protein (EWS) is known to be glycosylated by O-linked β-N-acetylglucosamine (O-GlcNAc), the dynamics and stoichiometry of its glycosylation remain obscure. Here, we report a dynamic change in EWS species during neuronal differentiation embryonic carcinoma P19 cells. Our findings suggest that O-GlcNAc participates regulation functions
A lectin from Amaranthus hypochondriacus var. Mexico (AHML) was purified by affinity chromatography using asialofetuin-Sepharose 4B. AHML is specific for N-acetyl-d-galactosamine as are the other lectins. has no carbohydrate moiety and requires metal ion hemagglutination activity. The pI of 6.8. a native molecular mass 45.0 kDa composed homo-subunits having masses 36.8 kDa.
Aiming at the application to photodynamic therapy, natural bacteriochlorophyll-a was converted chemically stable free-base derivatives possessing different kinds of hydrophilic C17-propionate residues. These semi-synthetic bacteriochlorins were found have self-assembling ability in an aqueous environment and formed J-type aggregates a cell culture medium containing 0.2% DMSO. The electronic absorption spectra all sensitizers showed Q