A5014564580- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Fungal and yeast genetics research
- Cancer-related Molecular Pathways
- Mitochondrial Function and Pathology
- Prostate Cancer Treatment and Research
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- DNA Repair Mechanisms
- 14-3-3 protein interactions
- Advanced Proteomics Techniques and Applications
- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
- ATP Synthase and ATPases Research
- RNA regulation and disease
- Polyamine Metabolism and Applications
- Epigenetics and DNA Methylation
- Cancer, Lipids, and Metabolism
- Nuclear Receptors and Signaling
- Genetic Neurodegenerative Diseases
- Cancer Cells and Metastasis
- Microtubule and mitosis dynamics
Xiamen University
2015-2025
Sanford Burnham Prebys Medical Discovery Institute
2011-2025
Center for Systems Biology
2013-2025
Westlake University
2023-2025
Klinikum rechts der Isar
2021-2023
Medical Genetics Center
2021-2023
Target (United States)
2020
Discovery Institute
2015-2019
Tyrolean Cancer Research Institute
2012
Innsbruck Medical University
2012
SCF ubiquitin ligases control various processes by marking regulatory proteins for ubiquitin-dependent proteolysis. To illuminate how complexes are regulated, we sought that interact with the human component CUL1. The COP9 signalosome (CSN), a suppressor of plant photomorphogenesis, associated multiple cullins and promoted cleavage ubiquitin-like protein NEDD8 from Schizosaccharomyces pombe CUL1 in vivo vitro. Multiple NEDD8-modified uniquely accumulated CSN-deficient S. cells. We propose...
Abstract Background Gene expression is controlled globally and at multiple levels in response to environmental stress, but the relationships among these dynamic regulatory changes are not clear. Here we analyzed global regulation during different stress conditions fission yeast, Schizosaccharomyces pombe , combining genome-wide data on mRNA, translation, protein profiles. Results We observed a strong overall concordance between mRNAs co-directional for both induced repressed genes, three...
Elongator is required for the synthesis of mcm(5)s(2) modification found on tRNAs recognizing AA-ending codons. In order to obtain a global picture role in translation, we used reverse protein arrays screen fission yeast proteome translation defects. Unexpectedly, this revealed that inactivation mainly affected three specific functional groups including proteins implicated cell division. The absence results delay mitosis onset and cytokinesis We demonstrate kinase Cdr2, which central...
As a ubiquitin-like modifier, ISG15 is conjugated to many cellular proteins in process termed protein ISGylation. However, the crosstalk between ISGylation and ubiquitin proteasome system not fully understood. Here, we report that substrate of Lys 29 on major acceptor site. Using model substrate, demonstrate can modify ubiquitin, which immobilized its form ISG15-ubiquitin mixed chains. Furthermore, our results indicate chains do serve as degradation signals for fusion substrate. Accordingly,...
Autoregulation is a control mechanism common to several proteins of the steroid/thyroid hormone receptor superfamily. In this work, effect androgens and antiandrogens on expression human androgen (hAR) in prostate breast cancer cell lines was studied. Northern blot analysis revealed decrease hAR steady state RNA levels LNCaP cells by 3.3 nM synthetic mibolerone. Maximal down-regulation 30% occurred 48 h after addition. T47D showed similar with mibolerone, while normal skin fibroblasts did...
Abstract Background PCI/MPN domain protein complexes comprise the 19S proteasome lid, COP9 signalosome (CSN), and eukaryotic translation initiation factor 3 (eIF3). The eIF3 complex is thought to be composed of essential core subunits required for global synthesis non-essential that may modulate mRNA specificity. Interactions unclear significance were reported between PCI proteins contained in CSN. Results Here, we report unexpected finding fission yeast has two distinct sharing common...
Abstract —During myocardial reperfusion, polymorphonuclear neutrophil (PMN) adhesion involving the intercellular molecule-1 (ICAM-1) may lead to aggravation and prolongation of reperfusion injury. We studied role early tumor necrosis factor-α (TNF-α) cleavage nuclear factor-κB (NF-κB) activation on ICAM-1 expression venular PMN in isolated hearts after ischemia (15 minutes) (30 480 minutes). NF-κB (electromobility shift assay) was found 30 minutes up 240 minutes. mRNA, assessed by Northern...
The cyclin-dependent kinase (CDK) inhibitor p27Kip1 is downregulated in a majority of human cancers due to ectopic proteolysis by the ubiquitin-proteasome pathway. expression p27 subject multiple mechanisms control involving several transcription factors, pathways and at least three different ubiquitin ligases (SCFSKP2, KPC, Pirh2), which regulate transcription, translation, protein stability subcellular localization. Using chemical genetics approach, we have asked whether this network can...
Instability of simple DNA repeats has been known as a common cause hereditary ataxias for over 20 years. Routine genetic diagnostics these phenotypically similar diseases still rely on an iterative workflow quantification repeat units by PCR-based methods limited precision. We established and validated clinical nanopore Cas9-targeted sequencing, amplification-free method simultaneous analysis 10 loci associated with clinically overlapping ataxias. The combines target enrichment CRISPR-Cas9,...