A5011871848- Circadian rhythm and melatonin
- Geological and Geochemical Analysis
- Spaceflight effects on biology
- Photoreceptor and optogenetics research
- High-pressure geophysics and materials
- Protein Kinase Regulation and GTPase Signaling
- earthquake and tectonic studies
- Geochemistry and Geologic Mapping
- Receptor Mechanisms and Signaling
- Neuroendocrine regulation and behavior
- Genetics, Aging, and Longevity in Model Organisms
- Sleep and Wakefulness Research
- Mineralogy and Gemology Studies
- Light effects on plants
- Cellular Mechanics and Interactions
- Physics of Superconductivity and Magnetism
- Neurobiology and Insect Physiology Research
- Neural dynamics and brain function
- Dietary Effects on Health
- Neural Networks and Applications
- Nerve injury and regeneration
- Connexins and lens biology
- Clay minerals and soil interactions
- stochastic dynamics and bifurcation
- Neurosurgical Procedures and Complications
Kansai University
2023-2024
Kyoto University
2013-2023
Shinshu University
1997-2023
Japan Science and Technology Agency
2015-2017
Hokkaido University
2003-2017
Kyoto Pharmaceutical University
2010-2015
Center for Non-Communicable Diseases
2015
Kyoto Prefectural University of Medicine
2011
Otsu Municipal Hospital
2011
RIKEN Advanced Science Institute
2010
Jet-lag symptoms arise from temporal misalignment between the internal circadian clock and external solar time. We found that rhythms of behavior (locomotor activity), gene expression, body temperature immediately reentrained to phase-shifted light-dark cycles in mice lacking vasopressin receptors V1a V1b (V1a(-/-)V1b(-/-)). Nevertheless, V1a(-/-)V1b(-/-) was still coupled clock, which oscillated normally under standard conditions. Experiments with suprachiasmatic nucleus (SCN) slices...
Synchronous oscillations of thousands cellular clocks in the suprachiasmatic nucleus (SCN), circadian centre, are coordinated by precisely timed cell–cell communication, principle which is largely unknown. Here we show that amount RGS16 (regulator G protein signalling 16), a known to inactivate Gαi, increases at selective time allow time-dependent activation intracellular cyclic AMP SCN. Gene ablation Rgs16 leads loss production cAMP and as result lengthens period behavioural rhythm. The...
The Rho family of small GTPases has been shown to be involved in the regulation neuronal morphology, and Rac exert antagonistic actions neurite formation. In this study, we have examined cross-talk between relation nerve growth factor (NGF)-induced outgrowth PC12 cells. NGF induced a rapid activation Rac1 suppression RhoA activity. Constitutively active RhoA, RhoAV14, or constitutively Gα12-induced endogenous inhibited NGF-induced without any effect on extracellular signal-regulated kinase...
In neuronal cells, activation of a certain heterotrimeric G protein-coupled receptor causes neurite retraction and cell rounding via the small GTPase Rho. However, specific proteins that mediate Rho-dependent have not yet been identified. Here we investigated effects expression constitutively active Gα subunits on morphology differentiated PC12 cells. Expression GTPase-deficient Gα<sub>12</sub>, Gα<sub>13</sub>, Gα<sub>q</sub>, but Gα<sub>i2</sub>, caused in These morphological changes...
The Rho family of small GTPases has been implicated in cytoskeletal reorganization and subsequent morphological changes various cell types. Among them, Rac Cdc42 have shown to be involved neurite outgrowth neuronal cells. In this study, we examined the role RhoG, another member GTPases, nerve growth factor (NGF)-induced PC12 Expression wild-type RhoG cells induced absence NGF, morphology RhoG-expressing was similar that NGF-differentiated Constitutively active RhoG-transfected extended short...
Body temperature in homeothermic animals does not remain constant but displays a regular circadian fluctuation within physiological range (e.g., 35°C–38.5°C mice), constituting fundamental systemic signal to harmonize clock-regulated physiology. Here, we find the minimal upstream open reading frame (uORF) encoded by 5′ UTR of mammalian core clock gene Per2 and reveal its role as regulatory module for temperature-dependent entrainment. A shift affect transcription instead increases...
Abstract G-protein-coupled receptors (GPCRs) are important drug targets with diverse therapeutic applications. However, there still more than a hundred orphan GPCRs, whose protein functions and biochemical features remain unidentified. Gpr176 encodes class-A GPCR that has role in circadian clock regulation mouse hypothalamus is also implicated human breast cancer transcriptional response. Here we show N -glycosylated. Peptide- -glycosidase treatment of extracts revealed endogenous undergoes...
Neurite outgrowth of PC12 cells is induced by nerve growth factor (NGF) but not epidermal (EGF). This differential response has been explained the duration mitogen-activated protein kinase (MAPK) activation; NGF induces sustained MAPK activation EGF leads short-lived activation. However, precise mechanisms have yet understood. Here we demonstrate difference between and in regulation Rac1, a small GTPase involved neurite outgrowth, cells. phosphoinositide 3-kinase dependently transient Rac1...
Abstract Liver metabolism undergoes robust circadian oscillations in gene expression and enzymatic activity essential for liver homeostasis, but whether the clock controls homeostatic self-renewal of hepatocytes is unknown. Here we show that hepatocyte polyploidization markedly accelerated around central vein, site permanent cell self-renewal, mice deficient Period genes. In these mice, a massive accumulation hyperpolyploid mononuclear binuclear occurs due to impaired mitogen-activated...
Abstract Non-coding cis -regulatory elements are essential determinants of development, but their exact impacts on behavior and physiology in adults remain elusive. Cis -element-based transcriptional regulation is believed to be crucial for generating circadian rhythms physiology. However, genetic evidence supporting this model based mutations the protein-coding sequences clock genes. Here, we report generation mutant mice carrying a mutation only at E′-box -element promoter region core gene...
Abstract Canonically, hormones are produced in the endocrine organs and delivered to target tissues. However, for steroids, concept of tissue intracrinology, whereby tissues where they exert their effect without release into circulation, has been proposed, but its role physiology/disease remains unclear. The meibomian glands eyelids produce oil prevent tear evaporation, which reduces with aging. Here, we demonstrate that (re)activation local intracrine activity through nicotinamide adenine...
Calcium signaling is pivotal to the circadian clockwork in suprachiasmatic nucleus (SCN), particularly rhythm entrainment environmental light-dark cycles. Here, we show that a small G-protein Gem, an endogenous inhibitor of high-voltage-activated voltage-dependent calcium channels (VDCCs), rapidly induced by light SCN neurons via (Ca2+)-mediated CREB/CRE transcriptional pathway. Gem attenuates light-induced through its interaction with VDCCs. The phase-shift magnitude locomotor activity...
The origin of silver doping effects on Y-Ba-Cu-O superconducting ceramics, such as a high-temperature shift the point at zero resistivity in measurements and an increase critical current density, was sought by transmission electron microscopy observations. Sintering strongly promoted together with suppression formation liquid phase grain boundaries silver. Consequently, phase, which is not superconductor, present almost all silver-doped while this pure ceramics.
Electron microprobe and micro-FTIR (mFTIR) analyses of a quartz crystal from hydrothermal vein reveal zoning in Al H concentrations. The concentration ranges 27 to 468 Al/106Si, the correspondingly 49 266 H/106Si. profile reveals positive correlation between At low (<100 Al/106 Si), H/Al ratio is ~1.0 infrared spectra show strong bands due Al-OH very weak Li-OH. These results indicate that most charge imbalance resulting Al3+ substituting for Si4+ compensated by H. an >100 Si, drops ~0.5...
The estrogen pathway plays an important role in the etiology of human endometrial carcinoma (EC). We examined whether biosynthesis tumor microenvironment promotes cancer. To examine contribution stromal cells to signaling EC, we used reporter stably transfected with response element (ERE) fused destabilized green fluorescent protein (GFP) gene. In this system, cancer from several patients activated ERE a variable extent. GFP expression level increased when testosterone, substrate for...
The glutamatergic neurotransmission in the suprachiasmatic nucleus (SCN) plays a central role entrainment of circadian rhythms to environmental light-dark cycles. Although effect operating via NMDAR (N-methyl D-aspartate receptor) is well elucidated, much less known about AMPAR (α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid entrainment. Here we show that, mouse SCN, GluR2 and GluR4 subtypes are abundantly expressed retinorecipient area. In vivo microinjection AMPA SCN during early...
To ensure the maintenance of tissues in mammals, cell loss must be balanced with production, proliferative activity being different from tissue to tissue. In this article, authors propose a new method for quantification activity, defined as S-phase fraction actively cycling cells, by dual labeling fluorescence and peroxidase immunohistochemistry using BrdU (marker S-phase) Ki67 antibodies G 1 -, S-, 2 M-phases) after one-step antigen retrieval. generative zones fundic pyloric glandular...
Abstract Gpr19 encodes an evolutionarily conserved orphan G-protein-coupled receptor (GPCR) with currently no established physiological role in vivo. We characterized expression the suprachiasmatic nucleus (SCN), locus of master circadian clock brain, and determined its context rhythm regulation. found that is mainly expressed dorsal part SCN, fluctuating a fashion. A cAMP-responsive element promoter was able to produce transcription SCN. −/− mice exhibited prolonged period delayed...
The Galpha subunits of the G(12) family heterotrimeric G proteins, defined by Galpha(12) and Galpha(13), have many cellular functions in common, such as stress fiber formation neurite retraction. However, a variety protein-coupled receptors appear to couple selectively Galpha(13). For example, thrombin lysophosphatidic acid (LPA) been shown induce via respectively. We recently showed that active forms Galpha(13) interact with Ser/Thr phosphatase type 5 through its tetratricopeptide repeat...