A5106133391- Receptor Mechanisms and Signaling
- Diabetes Treatment and Management
- Neuropeptides and Animal Physiology
- Protein Kinase Regulation and GTPase Signaling
- Pancreatic function and diabetes
- Cholesterol and Lipid Metabolism
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Biochemical Analysis and Sensing Techniques
- Eicosanoids and Hypertension Pharmacology
- Peroxisome Proliferator-Activated Receptors
- Cancer, Stress, Anesthesia, and Immune Response
- Diet, Metabolism, and Disease
- Renal Diseases and Glomerulopathies
- Pharmacological Effects and Assays
- Gene expression and cancer classification
- Metabolism, Diabetes, and Cancer
- Inflammatory mediators and NSAID effects
- Neuroscience of respiration and sleep
- Hormonal Regulation and Hypertension
- Fatty Acid Research and Health
- Liver Disease Diagnosis and Treatment
- Pharmacology and Obesity Treatment
Kyoto University
2016-2025
Tokyo Women's Medical University
2005-2023
Kyoto Pharmaceutical University
2012-2023
Kyoto University of Education
2020
National Center of Neurology and Psychiatry
2017
Toppan (Japan)
1998-2009
National Center For Child Health and Development
1999-2008
Commonwealth Scientific and Industrial Research Organisation
2008
Japan Science and Technology Agency
2008
Nagoya City University
2008
The maintenance of energy homeostasis is essential for life, and its dysregulation leads to a variety metabolic disorders. Under fed condition, mammals use glucose as the main fuel, short-chain fatty acids (SCFAs) produced by colonic bacterial fermentation dietary fiber also contribute significant proportion daily requirement. ketogenic conditions such starvation diabetes, ketone bodies in liver from are used sources. To balance intake, excess trigger an increase or decrease expenditure,...
Free fatty acids (FFAs) provide an important energy source and also act as signaling molecules. FFAs are known to exert a variety of physiological responses via their G protein-coupled receptors (GPCRs), such the GPR40 family. Recently, we identified novel FFA receptor, GPR120, that promotes secretion glucagon-like peptide-1 (Hirasawa, A., Tsumaya, K., Awaji, T., Katsuma, S., Adachi, Yamada, M., Sugimoto, Y., Miyazaki, Tsujimoto, G. (2005) Nat. Med. 11, 90-94). Here showed inhibit serum...
Gastric inhibitory polypeptide (GIP) is an incretin secreted from enteroendocrine K cells in response to meal ingestion. Recently free fatty acid receptor G protein-coupled (GPR) 120 was identified as a lipid sensor involved glucagon-like peptide-1 secretion. However, Gpr gene expression and its role remain unclear, partly due difficulties separation of other intestinal epithelial cells. In this study, we purified using GIP-green fluorescent protein (GFP) knock-in mice, which can be...
A weak, nonselective G protein-coupled receptor 120 (GPR120) agonist 10 was found by screening a series of carboxylic acids derived from the peroxisome proliferator-activated gamma (PPARgamma) 3. Modification based on homology model GPR120 led to first GPR120-selective 12. These results provide basis for constructing new tools probing biology and developing candidate therapeutic agents.
Synapsins are neuronal phosphoproteins that coat synaptic vesicles and believed to function in the regulation of neurotransmitter release. The signaling mechanism for short‐chain free fatty acid (SCFA)‐stimulated NE release was examined using primary‐cultured mouse sympathetic cervical ganglion neurons. Pharmacological knockdown experiments showed activation neurons by SCFA propionate involves receptor GPR41 linking Gβγ‐PLCβ3‐ERK1/2‐synapsin 2 signaling. Further, synapsin 2b directly...
GPR120 is a G protein-coupled receptor expressed preferentially in the intestinal tract and adipose tissue, that has been implicated mediating free fatty acid-stimulated glucagon-like peptide-1 (GLP-1) secretion. To develop GPR120-specific agonists, series of compounds (denoted as NCG compounds) derived from peroxisome proliferator-activated γ agonist were synthesized, their structure-activity relationships agonists explored. examine agonistic activities these newly synthesized compounds,...
Successful somatic cloned animal production has been reported in various domesticated species, including cattle; however, it is associated with a high rate of pregnancy failure. The low cloning yield could possibly arise from either an abnormal and/or poorly developed placenta. In comparison to control cows, fewer placentomes were found cell nuclear recipient (NT) cows at day 60 gestation, suggesting retardation fetal/placental growth these animals. NT not only had numbers chorionic villi...
Selective inactivation of α<sub>1B</sub>-adrenoceptor (AR) by the site-directed alkylating agent chlorethylclonidine (CEC) has been used as one major pharmacological criteria to subclassify α<sub>1</sub>-AR; however, mechanism for differential CEC sensitivity two subtypes is uncertain, and extent varies depending on treatment employed. In this study, we examined correlation between subcellular localization α<sub>1</sub>-AR (α<sub>1A</sub> α<sub>1B</sub>) sensitivity. Constructing tagged with...
We report the cloning and characterization of two isoforms human α 1C ‐adrenoceptor cDNA ( ‐2, C ‐3). These are generated by alternative splicing differ from clone we previously isolated ‐1) in their length sequences C‐terminal domain. Tissue distribution mRNAs showed that these variants co‐express with ‐1 heart, liver, cerebellum cerebrum. Despite structural differences, functional experiments transfected CHO cells three have similar ligand binding properties, all couple phospholipase C/Ca...
After fertilization, embryo development involves differentiation, as well of the fetal body and extra-embryonic tissues until moment implantation. During this period various cellular molecular changes take place with a genetic origin, e.g. elongation embryonic tissues, cell-cell contact between mother placentation. To identify profiles search for new candidate molecules involved during period, gene expression was analyzed custom designed utero-placental complementary DNA (cDNA) microarray....
Kisspeptin is a member of the RFamide neuropeptide family that implicated in gonadotropin secretion. Because kisspeptin-GPR54 signaling neuroendocrine regulation reproduction, GPR54 ligands represent promising therapeutic agents against endocrine secretion disorders. In present study, selectivity profiles agonist peptides were investigated for several GPCRs, including receptors. Kisspeptin-10 exhibited potent binding and activation FF receptors (NPFFR1 NPFFR2). contrast, short peptide...