A5011441043- Lipid metabolism and biosynthesis
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Peroxisome Proliferator-Activated Receptors
- Regulation of Appetite and Obesity
- Fatty Acid Research and Health
- Retinoids in leukemia and cellular processes
- Retinal Development and Disorders
- Meat and Animal Product Quality
- Cholesterol and Lipid Metabolism
- Protein Structure and Dynamics
- Neonatal Respiratory Health Research
- Receptor Mechanisms and Signaling
- Hematopoietic Stem Cell Transplantation
- Diet, Metabolism, and Disease
- Immune Cell Function and Interaction
- Effects of Environmental Stressors on Livestock
- Pharmacological Effects and Assays
- Liver physiology and pathology
- ATP Synthase and ATPases Research
- Multiple Myeloma Research and Treatments
- Mesenchymal stem cell research
- Plant biochemistry and biosynthesis
National Center for Global Health and Medicine
2013-2024
Nippon Medical School
2021-2024
Center for Global Health
2024
Tokyo Medical and Dental University
2020-2021
The University of Tokyo
2006-2021
Shinshu University
2003-2007
It has recently been discovered that G protein-coupled receptors (GPCR) 41 and 43 are characterized by having the short chain fatty acids acetate propionate as their ligands. The objective of this study was to investigate involvement GPCR41, GPCR43, ligands in process adipogenesis. We measured levels GPCR41 GPCR43 mRNA both adipose other tissues mouse. GRP43 expression higher four types tissue than tissues, whereas not detected any tissues. A high level found isolated adipocytes, but very...
All organisms consist of cells that are enclosed by a cell membrane containing bipolar lipids and proteins. Glycerophospholipids important not only as structural functional components cellular but also precursors various lipid mediators. Polyunsaturated fatty acids comprising arachidonic acid or eicosapentaenoic located at sn -2 position, -1 position glycerophospholipids in an asymmetrical manner. In addition to the asymmetry, diversity is for fluidity curvature. To explain distribution...
Polyunsaturated fatty acids (PUFAs) in phospholipids affect the physical properties of membranes, but it is unclear which biological processes are influenced by their regulation. For example, functions membrane arachidonate that independent a precursor role for eicosanoid synthesis remain largely unknown. Here, we show lack lysophosphatidylcholine acyltransferase 3 (LPCAT3) leads to drastic reductions levels, and LPCAT3-deficient mice neonatally lethal due an extensive triacylglycerol (TG)...
Phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine, PC), is an important constituent of biological membranes. It also the major component serum lipoproteins and pulmonary surfactant. In remodeling pathway PC biosynthesis, 1-acyl-sn-glycero-3-phosphocholine (LPC) converted to by acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT, EC 2.3.1.23). Whereas LPCAT activity has been detected in several tissues, structure detailed biochemical information on enzyme have not yet reported....
Recent research progress indicates a close link between ghrelin, natural ligand of GH secretagogues receptor (GHS-R), and both the metabolic balance body composition. To clarify involvement ghrelin GHS-R in process adipogenesis, we measured expression peroxisome proliferator-activated gamma 2 (PPAR-gamma 2) mRNA rat adipocytes using semiquantitative RT-PCR methods. The levels increased by up to 4-fold adipose tissue from epididymal parametrial regions as aged 4-20 wk were significantly...
Hematopoietic stem cells (HSCs) maintain lifelong hematopoiesis by remaining quiescent in the bone marrow niche. Recapitulation of a state culture has not been achieved, as rapidly proliferate and differentiate vitro. After exhaustive analysis different environmental factor combinations concentrations way to mimic physiological conditions, we were able engraftable HSCs for 1 month under very low cytokine concentrations, hypoxia, high fatty acid levels. Exogenous acids required likely due...
Abstract Background and Aims Chronic liver congestion reflecting right‐sided heart failure (RHF), Budd‐Chiari syndrome, or Fontan‐associated disease (FALD) is involved in fibrosis HCC. However, molecular mechanisms of HCC chronic remain poorly understood. Approach Results Here, we first demonstrated that promoted metastatic tumor growth using murine model by partial inferior vena cava ligation (pIVCL). As the initial step triggering promotion fibrosis, gut‐derived lipopolysaccharide (LPS)...
Abstract Exposure of testes to high-temperature environment results in defective spermatogenesis. Zebrafish exposed high temperature exhibited apoptosis not only germ cells but also Leydig cells, as expected from studies using mice or salmon. However, the role testicular somatic spermatogenesis defects remains unclear. We found that Trpv4 gene encoding sensitive ion channel was specifically upregulated temperature. High reduced hormone synthesis and led a prompt downregulation sperm...
Glycerophospholipids are structural and functional components of cellular membranes as well precursors various lipid mediators. Using acyl-CoAs donors, glycerophospholipids formed by the de novo pathway (Kennedy pathway) modified in remodeling (Lands' cycle). Various acyltransferases, including two lysophosphatidic acid acyltransferases (LPAATs), have been discovered from a 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family. Proteins this family contain putative acyltransferase...
Polyunsaturated fatty acids (PUFAs), such as docosahexaenoic acid (DHA) and arachidonic (ARA), play fundamental roles in mammalian physiology. Although PUFA imbalance causes various disorders, mechanisms of the regulation their systemic levels are poorly understood. Here, we report that hepatic DHA-containing phospholipids (DHA-PLs) determine PUFAs through SREBP1-mediated transcriptional program. We demonstrated liver-specific deletion Agpat3 leads to a decrease DHA-PLs compensatory increase...
Abstract Platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) plays a critical role in inflammatory disorders including experimental allergic encephalomyelitis (EAE), an animal model for multiple sclerosis (MS). Although PAF accumulation the spinal cord (SC) of EAE mice and cerebrospinal fluid MS patients has been reported, little is known about metabolic processing these diseases. In this study, we demonstrate that activities phospholipase A2 (PLA2)...
The factors that control fat deposition in adipose tissues are poorly understood. It is known visceral display a range of biochemical properties distinguish them from subcutaneous origin. However, we have little information on gene expression, either relation to or interspecies variation deposition. first step this study was identify genes expressed depot cattle using the differential RT-PCR method. Among transcripts identified as having expression two were cell division cycle 42 homolog...
ABSTRACT Adipose tissue is an important organ that involved in the peripheral regulation of body homeostasis, specifically, energy intake, storage and expenditure. Since fat metabolism utmost importance ruminants, signals mechanisms which regulate adiposity for fattening have been studied characterized by many different approaches. Adipogenesis adipose tissues ruminants triggered modulated several factors/genes, some (conjugated linoleic acid, tumor necrosis factor‐α adipogenin) examined...
Pulmonary surfactant, a complex composed primarily of lipids and associated proteins, is synthesized in alveolar type II (ATII) cells secreted into alveoli to prevent collapse during respiration. Although numerous studies have clarified the fundamental roles pulmonary molecular mechanisms transport secretion surfactant remain totally unknown. Thus, we screened candidate genes by comparing with expressed sequence tag (EST) libraries embryonic adult lungs using digital differential display...
Due to their high energy demands and characteristic morphology, retinal photoreceptor cells require a specialized lipid metabolism for survival function. Accordingly, dysregulation of leads the cell death degeneration. Mice bearing frameshift mutation in gene encoding lysophosphatidylcholine acyltransferase 1 (Lpcat1), which produces saturated phosphatidylcholine (PC) composed two fatty acids, has been reported cause spontaneous degeneration mice; however, mechanism by this affects is...