A5063237654- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Biomedical Ethics and Regulation
- Cancer Cells and Metastasis
- Renal and related cancers
- Cancer Research and Treatments
- Signaling Pathways in Disease
- Digestive system and related health
- Tissue Engineering and Regenerative Medicine
- Animal Genetics and Reproduction
- Helicobacter pylori-related gastroenterology studies
- Pancreatic function and diabetes
- Cancer-related gene regulation
- CAR-T cell therapy research
- RNA modifications and cancer
- 3D Printing in Biomedical Research
- Gastric Cancer Management and Outcomes
- Genetic factors in colorectal cancer
- Virus-based gene therapy research
- Reproductive Biology and Fertility
- Nuclear Receptors and Signaling
- RNA Interference and Gene Delivery
- Pancreatic and Hepatic Oncology Research
- Supramolecular Self-Assembly in Materials
- Cancer Genomics and Diagnostics
Kobe University
2015-2025
Kobe University Hospital
2015-2025
Kyoto University
2006-2022
Japan Science and Technology Agency
2008
Gladstone Institutes
2008
Induced pluripotent stem (iPS) cells have been generated from mouse and human fibroblasts by the retroviral transduction of four transcription factors. However, cell origins molecular mechanisms iPS induction remain elusive. This report describes generation adult hepatocytes gastric epithelial cells. These clones appear to be equivalent embryonic in gene expression are competent generate germline chimeras. Genetic lineage tracings show that liver-derived derived albumin-expressing No common...
Anacardic acid attenuates mutant TDP-43–associated abnormalities in motor neurons derived from ALS patient–specific induced pluripotent stem cells.
Hepatocytes generated from human induced pluripotent stem cells (hiPSCs) are unprecedented resources for pharmaceuticals and cell therapy. However, the in vitro directed differentiation of into mature hepatocytes remains challenging. Little attention has so far been paid to variations among hiPSC lines terms their hepatic differentiation. In current study, we developed an improved protocol compared 28 originated various somatic derived using retroviruses, Sendai viruses, or episomal...
Organelle targeting is a useful approach in drug development for cancer therapy. Peptide amphiphiles are good candidates specific organelles because they can be engineered into wide range of molecular structures, enabling customization functional needs. We have developed peptide amphiphile, C16‐(EY)3, that respond to tyrosine kinase activity and undergo phosphorylation inside cells. C16‐(EY)3 selectively induced apoptosis cells overexpressed kinase. The amphiphile self‐assembled nanofibers...
Cancer stem cells (CSCs) are considered to be responsible for the dismal prognosis of cancer patients. However, little is known about molecular mechanisms underlying acquisition and maintenance CSC properties in because their rarity clinical samples. We herein induced using defined factors. retrovirally introduced a set factors (OCT3/4, SOX2 KLF4) into human colon cells, followed by culture with conventional serum-containing medium, not embryonic cell medium. then evaluated cells. The...
Pituitary develops from oral ectoderm in contact with adjacent ventral hypothalamus. Impairment this process results congenital pituitary hypoplasia (CPH); however, there have been no human disease models for CPH thus far, prohibiting the elucidation of underlying mechanisms. In study, we established a model using patient-derived induced pluripotent stem cells (iPSCs) and 3D organoid technique, which hypothalamus develop simultaneously. Interestingly, patient iPSCs heterozygous mutation...
Pluripotent stem cells can be established by various methods, but they share several cytological properties, including germ cell differentiation in vitro, independently of their origin. Although mouse induced pluripotent (iPS) produce functional gametes vivo, it is still unclear whether or not have the ability to presumptive vitro. Here, we show that iPS derived from adult hepatocytes were able differentiate into marked vasa homolog (Mvh) expression feeder-free suspension cultures. Embryoid...
Self-assembly of synthetic molecules has been drawing broad attention as a novel emerging approach in drug discovery. Here, we report selective cell death induced by peptide amphiphile that self-assembles to form entangled nanofibers (hydrogel) based on intracellular pH (pHi). We found palmitoylated hexapeptide (C16-VVAEEE) formed hydrogel below 7. The formation the nanofibrous self-assembly was responsive small change around cytotoxicity C16-VVAEEE correlated with pHi cells. Microscope...
For augmentation or reconstruction of urinary bladder after cystectomy, urothelium derived from human induced pluripotent stem cells (hiPSCs) has recently received focus. However, previous studies have only shown the emergence expressing some urothelial markers among derivatives hiPSCs, and no report demonstrated stratified structure, which is a particularly important attribute barrier function mature urothelium. In present study, we developed method for directed differentiation hiPSCs into...
Late-onset hypogonadism (LOH) syndrome is characterized by age-related testosterone deficiency and negatively affects the quality of life older men. A promising therapeutic approach for LOH transplantation testosterone-producing Leydig-like cells (LLCs) derived from human induced pluripotent stem (hiPSCs). However, previous studies have encountered obstacles, such as limited cell longevity, insufficient production, inefficiency differentiation. To address these issues, we developed a novel...
Abstract In the present study, we successfully generated lung cancer stem cell (CSC)-like cells by introducing a small set of transcription factors into line. addition to properties that are conventionally referred as CSC properties, induced CSCs exhibited ability form cancer-like tissues in vitro with vascular and mesenchymal cells, which showed structures immunohistological patterns were similar human tissues. We named them “lung organoids”. found interleukin-6 (IL-6), was expressed CSCs,...
Intestinal metaplasia is related to gastric carcinogenesis. Previous studies have suggested the important role of CDX2 in intestinal metaplasia, and several reports shown that overexpression mouse mucosa caused metaplasia. However, no study has examined induction using human mucosa. In present study, produce an model vitro, we differentiated human-induced pluripotent stem cells (hiPSC) organoids, followed by a tet-on system. The induced, although not completely, phenotypes enhanced...
Cell-selective killing using molecular self-assemblies is an emerging concept for cancer therapy. Reported are triggered by hydrolysis of well-designed molecules inside or outside cells. This can occur in and normal cells because the abundance water living systems. Here, we report situ synthesis a self-assembling molecule tyrosine kinase overexpressed We designed tyrosine-containing peptide amphiphile (C16-E4Y) that transformed into phosphorylated (C16-E4pY) kinase. Phosphorylation C16-E4Y...
The chorioallantoic membrane (CAM) assay is a well-established technique to evaluate tumor invasion and angiogenesis may overcome the shortcoming of patient-derived xenograft (PDX) mouse model. Currently, few reports have described lung cancer in CAM assay. We therefore used evaluation cancer.Lung cell line-derived organoids or lines were transplanted into on embryonic development day (EDD) 10, an analysis was performed EDD 15. Microscopic macroscopic images movies grafts captured analyzed....
To make sure that novel human cell-based products contribute to health care, it is essential that, based on sound science at present, suitable measures be taken by the manufacturers and regulatory authorities applying these treatment of patients taking into account specificity starting cell lines, manufacturing process, products, administration procedures, diseases in question, patient population. As part such an endeavor, we studied scientific principles, concepts, basic technical elements...
For a long time, ex vivo-expanded peripheral-blood-derived γδT cell (PBγδT)-based immunotherapy has been attractive, and clinical trials have undertaken. However, the difficulty in expanding cytotoxic cells to an adequate number major limitation efficacy of treatment most cases. We successfully re-generated from cell-derived human induced pluripotent stem (iPSCs). The iPSC-derived (iγδTs) killed several cancer types histocompatibility complex (MHC)-unrestricted manner. Single-cell RNA...
Our study and several studies have reported that in some cancers, including pancreatic ductal adenocarcinoma (PDAC), the expression of squamous lineage markers, such as esophagus-tissue-specific genes, correlated with a poor prognosis. However, mechanism by which acquisition phenotypes leads to prognosis remains unclear. We previously retinoic acid signaling via receptor γ (RARγ signaling) determines differentiation into esophageal epithelium. These findings hypothesized activation RARγ...
Abstract γδT cells constitute a small proportion of lymphocytes in peripheral blood. Unlike αβT cells, the anti-tumor activities are exerted through several different pathways MHC-unrestricted manner. Thus, immunotherapy using is considered to be effective for various types cancer. Occasionally, however, ex vivo expanded not as expected due cell exhaustion. To overcome issue T-cell exhaustion, researchers have generated induced pluripotent stem (iPSCs) that harbor same receptor (TCR) genes...