A5102888678- Alzheimer's disease research and treatments
- Cholinesterase and Neurodegenerative Diseases
- Computational Drug Discovery Methods
- Schizophrenia research and treatment
- Pluripotent Stem Cells Research
- 14-3-3 protein interactions
- CRISPR and Genetic Engineering
- Down syndrome and intellectual disability research
- RNA Interference and Gene Delivery
- Drug Transport and Resistance Mechanisms
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Obsessive-Compulsive Spectrum Disorders
- Vestibular and auditory disorders
- Signaling Pathways in Disease
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Nitric Oxide and Endothelin Effects
- Metabolism and Genetic Disorders
- Alcoholism and Thiamine Deficiency
- Cancer-related molecular mechanisms research
- Bipolar Disorder and Treatment
- Ophthalmology and Eye Disorders
- Protein Structure and Dynamics
- Medicinal Plants and Neuroprotection
- Neuroendocrine regulation and behavior
Tokyo University of Science
2003-2024
Yokohama University of Pharmacy
2020-2023
Nagasaki University
2012-2021
Science and Technology Corporation (United States)
2017
Centre de Recherche en Économie et Statistique
2017
Centre for Research in Engineering Surface Technology
2017
MRC Laboratory of Molecular Biology
2013
Saitama Medical University
2005-2013
Japan Science and Technology Agency
2011-2013
RIKEN Center for Brain Science
2005-2011
We investigated the effects of interleukin-1 beta (IL-1 beta), administered directly into rat anterior hypothalamus (AHY), on monoamine release in same region by using a brain microdialysis technique and an HPLC-electrochemical detection system. First, to study local IL-1 beta, we used probe equipped with microinjection tube for administering which had been inserted. (1 ng) injected AHY elicited norepinephrine (NE), dopamine (DA), 5-HT, as well increases their metabolites,...
We investigated whether interleukin-1 (IL-1) activity in the rat hypothalamus was increased by immobilization stress (IS), and pretreatment with an receptor antagonist (IL-1Ra) is capable of inhibiting IS-induced elevations hypothalamic norepinephrine (NE), dopamine (DA), serotonin (5-HT) levels their metabolites as well plasma adrenocorticotropic hormone (ACTH). IL-1 estimated a bioassay using mouse thymocyte proliferation presence concanavalin A. IL-1Ra administered directly into anterior...
Background Alzheimer's disease (AD) is a neurodegenerative disorder that causes progressive memory and cognitive decline during middle to late adult life. The AD brain characterized by deposition of amyloid β peptide (Aβ), which produced from precursor protein β- γ-secretase (presenilin complex)-mediated sequential cleavage. Induced pluripotent stem (iPS) cells potentially provide an opportunity generate human cell-based model would be crucial for drug discovery as well investigating...
Abstract Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the accumulation of amyloid plaques and neurofibrillary tangles in brain. The major component plaques, β peptide (Aβ), generated from precursor protein (APP) β‐ γ‐secretase‐mediated cleavage. Because β‐secretase/beta‐site APP cleaving enzyme 1 (BACE1) knockout mice produce much less Aβ grow normally, β‐secretase inhibitor thought to be one most attractive targets for development therapeutic interventions AD...
Accumulation of amyloid-β peptide (Aβ) in the brain is closely associated with cognitive decline Alzheimer's disease (AD). Stereotaxic infusion neprilysin-encoding viral vectors into hippocampus has been shown to decrease Aβ AD-model mice, but more efficient and global delivery necessary treat broadly distributed burden AD. Here we developed an adeno-associated virus (AAV) vector capable providing neuronal gene expression throughout brains after peripheral administration. A single...
Abstract A neuropathological hallmark of Alzheimer's disease is the presence amyloid plaques in brain. Amyloid‐β peptide (Aβ) major constituent and generated by proteolytic cleavages precursor protein (APP) β‐ γ‐secretases. Growing evidence shows that lipid rafts are critically involved regulating Aβ generation. In support this, APP, Aβ, presenilins have been found rafts. Although cholesterol plays a crucial role maintaining rafts, functions other components generation unknown. Caveolins...
γ-Secretase catalyzes the cleavage of intramembrane region Alzheimer amyloid precursor protein (APP), generating p3, amyloid-β peptide (Aβ), and APP intracellular domain (AICD). Although a γ-secretase inhibitor has been shown to cause an accumulation C-terminal fragments (CTFs) α β decrease levels p3 or Aβ AICD, we found that treatment with lysosomotropic weak base, such as chloroquine ammonium chloride, caused simultaneous both CTFs suggesting lysosomal proteases are also involved in...
In order to define the acute effects of a moderate quantity alcohol on balance, related vestibular function, vestibulo-ocular reflex (VOR) test, caloric test and dynamic posturography (EquiTest) were performed. Ten healthy male volunteers aged 19-27 average 22.8) years old imbibed 1.5 ml whisky (alcohol content 43%) per kilogram body weight within 5 min. Blood level (BAL) was measured before administration then after 30, 90, 150 Equilibrium examinations performed immediately each blood...
Amyloid-β peptide (Aβ) accumulation is a triggering event leading to the Alzheimer's disease (AD) pathological cascade. Almost all familial AD-linked gene mutations increase Aβ production and accelerate onset of AD. The Swedish mutation amyloid precursor protein (APP) affects β-secretase activity increases up ca. 6-fold in cultured cells; age around 50. Down syndrome (DS) patients with chromosome 21 trisomy present AD-like pathologies at earlier ages (40s) compared sporadic AD patients,...
Down syndrome (DS), the most common genetic disorder, is caused by trisomy 21. DS accompanied heart defects, hearing and vision problems, obesity, leukemia, other conditions, including Alzheimer's disease (AD). In comparison, cancers are rare in people with DS. Overexpression of dual specificity tyrosine-phosphorylation-regulated kinase 1A a regulator calcineurin 1 located on chromosome 21 leads to excessive suppression calcineurin-nuclear factor activated T cells (NFAT) signaling pathway,...
Tardive dyskinesia (TD) is a therapy-resistant adverse effect of neuroleptics. Although the exact pathophysiology TD unknown, oxygen radicals have been speculated to play role in based on several lines evidence. Superoxide dismutase (SOD) key enzyme which scavenges radicals. The authors investigated association between erythrocyte SOD activity and TD.Erythrocyte activities were measured, blinded as presence or absence TD. In 30 patients with schizophrenia who had typical neuroleptics for...
We developed a simplified and sensitive method to identify Alzheimer's disease (AD) biomarker candidates by quantitative targeted proteomic analysis (combination of liquid chromatography tandem mass spectrometry multiplexed-multiple reaction monitoring/selected monitoring analysis) culture media from neurons differentiated induced pluripotent stem cells (iPSCs) established AD patients. found that alpha-1-acid glycoprotein (ORM1) was decreased in the AD-iPSC–derived neurons, consistent with...
GGAA motifs in the human
Ono Y, Yoshimura K, Sueoka R, Yamauchi Mizushima H, Momose T, Nakamura Okonogi Asai M. Avoidant personality disorder and taijin kvoufu: Sociocultural implications of the WHO/ ADAMHA International Study Personality Disorders in Japan. Acta Psychiatr Scand 1996: 93: 172–176. © Munksgaard 1996. This paper discusses characteristics avoidant a cultural context based on Japanese concept kyoufu as well that DSM‐III‐R DSM‐IV Social phobia. Sixty‐six patients were given Disorder Examination...
ABSTRACT The computed tomography (CT) scans of 46 chronic schizophrenic patients and controls were studied using ventricular‐brain ratio. Ewans’ index, cella media index. None the indices used revealed significant differences between patient control groups.
Parental bonding patterns were studied in 52 female Japanese eating disorder outpatients with and without histories of sexual or physical abuse dissociation. Instruments included the Bonding Instrument (PBI), Dissociative Experiences Scale (DES) Disorders Interview Schedule (DDIS). Those history, but not had significantly different parental scores higher DES compared subjects abuse. PBI correlated. Although was useful discriminating between those histories, it did detect differences degree...