A5108122716- Cellular Mechanics and Interactions
- Prion Diseases and Protein Misfolding
- Tendon Structure and Treatment
- Bone Tissue Engineering Materials
- Collagen: Extraction and Characterization
- Advanced Fluorescence Microscopy Techniques
- 3D Printing in Biomedical Research
- Textile materials and evaluations
- Alcoholism and Thiamine Deficiency
- Dermatologic Treatments and Research
- Neurological diseases and metabolism
- Spectroscopy Techniques in Biomedical and Chemical Research
- Wound Healing and Treatments
- Spaceflight effects on biology
- Amino Acid Enzymes and Metabolism
- Electrospun Nanofibers in Biomedical Applications
- Ion channel regulation and function
- Tissue Engineering and Regenerative Medicine
- T-cell and Retrovirus Studies
- Elasticity and Material Modeling
- Soil Mechanics and Vehicle Dynamics
- Hydrogels: synthesis, properties, applications
- Advancements in Transdermal Drug Delivery
- Antibiotics Pharmacokinetics and Efficacy
- Gait Recognition and Analysis
Tokushima University
2013-2025
Kobe University
2003-2025
Gifu University
2024
Nagasaki University
1975-2023
Yokohama National University
2019-2020
Yamaguchi University
2008-2011
Niigata University of Pharmacy and Medical and Life Sciences
1996
Tohoku University
1976
Late-onset hypogonadism (LOH) syndrome is characterized by age-related testosterone deficiency and negatively affects the quality of life older men. A promising therapeutic approach for LOH transplantation testosterone-producing Leydig-like cells (LLCs) derived from human induced pluripotent stem (hiPSCs). However, previous studies have encountered obstacles, such as limited cell longevity, insufficient production, inefficiency differentiation. To address these issues, we developed a novel...
Keloids most commonly develop in the regions where skin is constantly stretched. Although some keloid-derived fibroblasts exhibit higher single calcium spikes than normal dermal during short-time cyclical stretching, signal responses to long-time stretching remain unclear. This study compared intracellular Ca2+ dynamics induced by stimuli between control group (normal fibroblasts) and keloid (keloid-derived fibroblasts). Each was cyclically exposed a two-dimensional stretch (10% strain). A...
In osteoblast cells, cells change their intracellular Ca2+ concentration ([Ca2+]i) as the response to mechanical stimuli. Although it has been reported that responded many kinds of stimuli including stretch substrate, shear stress in fluid flow, direct indentation glass microneedle and hydrostatic pressure etc., detail characteristics calcium signaling substrate still remains unclear because motion artifact during application causes out focus plane observation area, complicates situ time...
In bone tissue engineering and regeneration, there is a considerable need for an unstained method of monitoring collagen fibers produced by osteoblasts. This because play important role as matrix continuous their temporal dynamics in clarifying the organization process toward forming tissue. work described here, using second-harmonic-generation (SHG) microscope, we performed situ time-series cultured osteoblasts without staining. Use 19 fs near-infrared pulsed light enables us to visualize...
The aim of this study is to evaluate the osteoblastic collagen synthesis under mechanical stimulation using second-harmonic-generation (SHG) microscopy. We apply SHG microscopy monitor fibers synthesized by osteoblast-like cells (MC3T3-E1) without need for fixation and staining. To quantitatively influence on synthesis, we compare images osteoblast-synthesized with a cyclic stretch stimulus applied lab-made stretching device. acquire every 7 days 3 weeks at different conditions (5 min/day...
The absorption of methochlorpromazine in rat small intestinal everted sac was investigated. 0.22% the drug added mucosal fluid transferred to serosal for 30 min at 37°C. slightly inhibited by choline, and more markedly hydrophobic quaternary ammonium cations such as tetraethylammonium cetyltrimethylammonium. Moderate inhibition observed a polyamine, spermine. rate depended on temperature. Arrhenius plot apparent transfer constant revealed high activation energy (117 kJ/mol) transport. Uptake...
In this study, we used Raman spectroscopy to observe the process of osteoblast calcification under microvibration stimulation. Osteoblast cultures were prepared three conditions: control group was cultured statically without vibration stimulation, and 45-Hz 90-Hz stimulated by at 45 Hz 90 Hz, respectively, for 30 minutes per day with an acceleration amplitude 0.2 G. The incubation periods 7, 9, 11 14 days, observations conducted using after fixation. group, accelerated. This produced a large...
Interferon regulatory factor 4 (IRF4) is a crucial transcription that plays vital role in lymphocyte development, including the fate-determining steps terminal differentiation. It also implicated development of lymphoid tumors such as multiple myeloma and adult T-cell leukemia. IRF4 can form homodimer heterocomplexes with other factors purine-rich box1 activator protein 1. Each complex binds to specific DNA sequences regulate distinct set genes. However, precise relationship among these...
Congenital adrenal hyperplasia (CAH) causes hypogonadotropic hypogonadism due to the excessive production of androgens, which results in hypospermatogenesis some male patients. We herein present a CAH case with and infertility. A 26-year-old receiving steroid therapy for 21 hydroxylase deficiency was diagnosed low gonadotropin levels, an elevated ACTH level, severe oligozoospermia. The switching from hydrocortisone dexamethasone resulted normalization levels semen findings. couple underwent...
Creutzfeldt-Jakob disease (CJD) is an important dementia disorder. However, clinical diagnosis can be difficult and delayed for many primary physicians caring patients. The aim of the present study was to describe neuropathological results individual with CJD who seen by a community hospital. Our report may inform on understanding significance CJD.Clinical information obtained from medical records. Neuropathological biochemical analyses were performed using autopsied brain.A 58-year-old...
Title A Single-Nucleotide Polymorphism of PARK2 Affects the Phenotype in Sporadic Parkinson Disease Author(s) Sakai, Muniko; Tsujino, Akira; Eguchi, Hiroto; Sato, Katsuya; Shirabe, Susumu; Tateishi, Yohei; Tsujihata, Mitsuhiro; Yoshimura, Toshiro; Katsumi Citation Acta medica Nagasakiensia, 54(3), pp.67-71 ; 2010 Issue Date 2010-03 URL http://hdl.handle.net/10069/23120 Right NAOSITE: Nagasaki University's Academic Output SITE
Dynamic behavior ofstress fibers in Qsteoblastic cell induced by
Introduction Neurogenic overactive bladder is a main feature of human T‐cell leukemia virus type 1‐associated myelopathy/tropical spastic paraparesis. We successfully performed intravesical onabotulinumtoxinA therapy for refractory neurogenic due to Case presentation retrospectively reviewed four patients with paraparesis who underwent wall injections from April October 2020. All were female. Their median age was 66 (range, 63–71) years. They previously treated β3‐adrenergic receptor...
Osteoblast-produced collagen matrix in bone is influenced by the mechanical stimulus from their surroundings. However, it has been still unclear how affects production osteoblasts. Therefore, strongly required to investigate characteristics of osteoblastic regenerative tissue engineering. Recently, second-harmonic-generation (SHG) microscope attracted attention for situ visualization fiber because less invasiveness, unstaining and no fixation, as well high spatial resolution 3D imaging....
Abstract Convulsive epileptic seizures are rare in Creutzfeldt‐Jakob disease (CJD), and their clinical EEG features have not been reported detail. We describe a case of familial CJD with an E200K mutation the prion protein who presented bilateral tonic–clonic (BTCS) during long‐term video‐EEG monitoring. Semiologically, BTCS showed focal signs such as head turning eye deviation to left. The ictal started generalized polyspikes. Interictal periodic discharges right fronto‐temporal...