A5070169684- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Autophagy in Disease and Therapy
- Microtubule and mitosis dynamics
- Cell Adhesion Molecules Research
- Cell death mechanisms and regulation
- Biocrusts and Microbial Ecology
- Cardiomyopathy and Myosin Studies
- Phagocytosis and Immune Regulation
- Heat shock proteins research
- Force Microscopy Techniques and Applications
- Skin and Cellular Biology Research
- Planarian Biology and Electrostimulation
- Muscle Physiology and Disorders
- Adenosine and Purinergic Signaling
- Energy and Environment Impacts
- Calcium signaling and nucleotide metabolism
- Advanced Fluorescence Microscopy Techniques
- Cannabis and Cannabinoid Research
- Caveolin-1 and cellular processes
- Cancer-related Molecular Pathways
- Protist diversity and phylogeny
- Alzheimer's disease research and treatments
- Spaceflight effects on biology
- Algal biology and biofuel production
Tokyo Medical and Dental University
2014-2023
Japan Science and Technology Agency
2011-2013
Osaka University
2001-2013
RIKEN Center for Computational Science
2012
Ube Frontier University
2011
National Institute of Advanced Industrial Science and Technology
2008
University of Dundee
2003-2007
Kyoto University
1999-2004
Wellcome Trust
2004
Imperial College London
2004
Contraction of the cortical actin cytoskeleton underlies both rear retraction in directed cell migration and cytokinesis. Here, we show that talin, a central component focal adhesions, has major role these processes. We found Dictyostelium talin A colocalized with myosin II migrating cells cleavage furrow. During migration, A-null displayed long thin tail devoid filaments, whereas additional depletion SibA, transmembrane adhesion molecule binds to A, reverted this phenotype, suggesting...
Actin and myosin II play major roles in cell migration. Whereas pseudopod extension by actin polymerization has been intensively researched, less attention paid to how the rest of cytoskeleton such as cortex contributes In this study, cortical filaments were simultaneously observed migrating Dictyostelium cells under total internal reflection fluorescence microscopy. The remained stationary with respect substratum advanced. However, recovery after photobleaching experiments direct...
Autophagy is an evolutionarily conserved process that degrades subcellular constituents. Mammalian cells undergo two types of autophagy; Atg5-dependent conventional autophagy and Atg5-independent alternative autophagy, the molecules required for latter type are largely unknown. In this study, we analyzed molecular mechanisms genotoxic stress-induced identified essential role p53 damage-regulated modulator (Dram1). Dram1 was sufficient to induce autophagy. mechanism functions in closure...
Talin is a cytoskeletal protein involved in constructing and regulating focal adhesions animal cells. The cellular slime mold Dictyostelium discoideum has two talin homologues, talA talB, earlier studies have characterized the single knockout mutants. talA(-) cells show reduced adhesion to substrates slightly impaired cytokinesis leading high proportion of multinucleated vegetative stage, while development normal. In contrast, talB(-) are by motility developmental they arrested at...
The Dictyostelium stalk cell inducer differentiation-inducing factor (DIF) directs tyrosine phosphorylation and nuclear accumulation of the STAT (signal transducer activator transcription) protein Dd-STATc. We show that hyperosmotic stress, heat shock oxidative stress also activate Hyperosmotic is known to elevate intracellular cGMP cAMP levels, membrane-permeant analogue 8-bromo-cGMP rapidly activates Dd-STATc, whereas 8-bromo-cAMP a much less effective inducer. Surprisingly,however,...
Cell migration is a process crucial for variety of biological events, such as morphogenesis and wound healing. Several reports have described the possible regulation cell by autophagy; however, this remains controversial. We here demonstrate that mouse embryonic fibroblasts (MEFs) lacking autophagy protein 5 (Atg5), an essential molecule autophagy, moved faster than wild-type (WT) MEFs. Similar results were obtained MEFs Atg7 unc-51-like kinase 1 (Ulk1), which are molecules required...
Dynamin has been proposed to play an important role in cytokinesis, although the nature of its contribution remained unclear. Dictyostelium discoideum five dynamin‐like proteins: DymA, DymB, DlpA, DlpB and DlpC. Cells mutant for dymA , dlpA or dlpB presented defects cytokinesis that resulted multinucleation when cells were cultured suspension. However, could divide normally attached substratum; this latter process depends on traction‐mediated B. A dynamin GTP ase inhibitor also blocked...
FERM domain proteins, including talins, ERMs, FAK and certain myosins, regulate connections between the plasma membrane, cytoskeleton extracellular matrix. Here we show that FrmA, a Dictyostelium discoideum protein containing two talin-like domains, plays major role in normal cell shape, cell-substrate adhesion actin organisation. Using total internal reflection fluorescence (TIRF) microscopy FrmA-null cells are more adherent to substrate than wild-type because of an increased number,...
Dictyostelium, the only known non-metazoan organism to employ SH2 domain:phosphotyrosine signaling, possesses STATs (signal transducers and activators of transcription) protein kinases with orthodox domains. Here, however, we describe a novel Dictyostelium STAT containing remarkably divergent domain. Dd-STATb displays 15 amino acid insertion in its domain conserved essential arginine residue, which interacts phosphotyrosine all other domains, is substituted by leucine. Despite these...
Dynamin is a large GTPase responsible for diverse cellular processes, such as endocytosis, division of organelles, and cytokinesis. The social amoebozoan, Dictyostelium discoideum, has five dynamin-like proteins: dymA, dymB, dlpA, dlpB, dlpC. DymA, or dlpB-deficient cells exhibited defects in DlpA dlpB were found to colocalize at cleavage furrows from the early phase, dymA localized intercellular bridge connecting two daughter cells, indicating that these dynamins contribute cytokinesis...
ABSTRACT ecmB and mrrA are expressed in the cups that cradle Dictyostelium spore heads, MybE is necessary for their expression lower but not upper cup cells. A Myb site within promoter both cups. Thus, multiple proteins required ancillary stalk differentiation.
Abstract Numerous studies have investigated the various cellular responses against genotoxic stress, including those mediated by focal adhesions. We here identified a novel type of adhesion remodelling that occurs under stress conditions, which involves replacement active kinase (FAK) with FAK-related non-kinase (FRNK). FRNK stabilized adhesions, leading to strong cell-matrix adhesion, and FRNK-depleted cells were easily detached from extracellular matrix upon stress. This occurred in wide...
The spatial expression patterns of genes involved in cyclic adenosine monophosphate (cAMP) responses during morphogenesis Dictyostelium discoideum were analyzed by situ hybridization. Genes encoding adenylyl cyclase A (ACA), cAMP receptor 1, G-protein alpha2 and beta subunits, cytosolic activator ACA (CRAC Aimless), catalytic subunit protein kinase (PKA-C) phosphodiesterases (PDE REG-A) preferentially expressed the anterior prestalk (tip) region slugs, which acts as an organizing center. MAP...
Although the distinct distribution of certain molecules along anterior or posterior edge is essential for directed cell migration, mechanisms to maintain asymmetric protein localization have not yet been fully elucidated. Here, we studied a mechanism localizations two Dictyostelium talin homologues, A and B, both which play important roles in migration adhesion. Using GFP fusion, found that as well its C-terminal actin-binding region, consists an I/LWEQ domain villin headpiece domain, was...
We found novel development rescuing factors (DRFs) secreted from developing Dictyostelium cells, by using a mutant (erkB−) which is missing MAP-kinase ERK2 as test strain for bioassay. The erkB− fails to undergo multicellular morphogenesis due impaired cAMP signaling. However, such developmental defect can be restored the presence of low-molecular weight DRFs that are wild-type cells. previously showed DIF-1 (Differentiation-Inducing Factor 1 stalk cells) possesses this activity, indicating...