A5031242190- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Carcinogens and Genotoxicity Assessment
- Radiation Dose and Imaging
- Ubiquitin and proteasome pathways
- DNA and Nucleic Acid Chemistry
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- PARP inhibition in cancer therapy
- Acute Lymphoblastic Leukemia research
- Effects of Radiation Exposure
- Retinoids in leukemia and cellular processes
- RNA modifications and cancer
- Heme Oxygenase-1 and Carbon Monoxide
- Chronic Lymphocytic Leukemia Research
- Nuclear Structure and Function
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Acute Myeloid Leukemia Research
- Mitochondrial Function and Pathology
- Radioactive contamination and transfer
- Cancer therapeutics and mechanisms
- Genomics, phytochemicals, and oxidative stress
- Cardiac electrophysiology and arrhythmias
Hiroshima University
2015-2024
Science Council of Japan
2022
Research Institute for Bioscience and Biotechnology
2006-2019
Kyowa Kirin (Japan)
2017
RIKEN Center for Integrative Medical Sciences
2016
Japanese Red Cross Nagoya Daini Hospital
2016
Hiroshima Minami High School
2015
Bioengineering Center
2014
Itron (United States)
2014
National Cancer Registry
2014
Chromatin reorganization plays an important role in DNA repair, apoptosis, and cell cycle checkpoints. Among proteins involved chromatin reorganization, TIP60 histone acetyltransferase has been shown to play a repair apoptosis. However, how regulates the response of human cells damage is largely unknown. Here, we show that ionizing irradiation induces acetylation H2AX, variant form H2A known be phosphorylated following damage. Furthermore, ubiquitination H2AX via ubiquitin-conjugating enzyme...
Small Maf proteins serve as dual-function transcription factors through an exchange of their heterodimerization partners. For example, heterodimers with hematopoietic cell-specific p45 NF-E2 or NF-E2-related (Nrf), they activate the β-globin antioxidative stress enzyme heme oxygenase 1 (HO-1) genes, respectively. In contrast, together Bach1, repress these same genes. However, signals leading to this partner are not known. Using chromatin immunoprecipitation assays in NIH 3T3 cells, we show...
Whether chromosomes maintain their nuclear positions during interphase and from one cell cycle to the next has been controversially discussed. To address this question, we performed long-term live-cell studies using a HeLa line with GFP-tagged chromatin. Positional changes of intensity gravity centers fluorescently labeled chromosome territories (CTs) on order several μm were observed in early G1, suggesting role CT mobility establishing architecture. Thereafter, highly constrained within...
Rad51, a eukaryotic RecA homologue, plays central role in homologous recombinational repair of DNA double-strand breaks (DSBs) yeast and is conserved from to human. Rad51 shows punctuate nuclear localization human cells, called foci, typically during the S phase (Tashiro, S., N. Kotomura, A. Shinohara, K. Tanaka, Ueda, Kamada. 1996. Oncogene. 12:2165–2170). However, topological relationships that exist nuclei between foci damaged chromatin have not been studied thus far. Here, we report on...
Mutations of the Glu76 residue canonical histone H2B are frequently found in cancer cells. However, it is quite mysterious how a single amino acid substitution one multiple genes affects cell fate. Here we that E76K mutation, which replaced by Lys (E76K), distorted interface between and H4 nucleosome, as revealed crystal structure induced nucleosome instability vivo vitro. Exogenous production mutant robustly enhanced colony formation ability expressing cells, indicating has potential to...
Background Although the National Lung Screening Trial reported a significant reduction in lung cancer mortality when low-dose (LD) CT chest examinations are used for diagnosis, their biologic effects from radiation exposure remain unclear. Purpose To compare LD and standard-dose (SD) DNA double-strand breaks chromosome aberrations (CAs) peripheral blood lymphocytes. Materials Methods Between March 2016 June 2018, 209 participants who were referred to respiratory surgery department studies...
Abstract Cigarette smoking is a major risk factor for atherosclerosis. We previously reported that DNA damage was accumulated in atherosclerotic plaque, and increased human mononuclear cells by smoking. As vascular endothelial are known to modulate inflammation, we investigated the mechanism which activates innate immunity focusing on damage. Furthermore, sought characterize plasma level of cell-free (cfDNA), result mitochondrial and/or genomic damage, as biomarker smoke extract (CSE)...
The export of certain nuclear proteins is involved in the regulation various functions, including transcription. In some cases, target induced upon environmental or cellular cues, resulting conditional gene expression. small Maf appear to be critical regulators heme oxygenase (HO)-1, an anti-oxidant defense enzyme that degrades into iron, carbon monoxide, and biliverdin. Although ho-1 repressed by Bach1/small heterodimers, it activated Nrf2/small indicating Bach1 Nrf2 compete with each...
Bach2 is a B cell-specific transcription repressor whose deficiency in mice causes reduced class switch recombination and somatic hypermutation of immunoglobulin genes. Little known about the direct target genes cells. By analyzing various cell plasma lines, we showed that expression patterns Blimp-1 (B lymphocyte-induced maturation protein 1), master regulator differentiation, are mutually exclusive. The reporter gene (Prdm1) was repressed by overexpression lines. heterodimer Bach2/MafK...
Genetic information encoded in chromosomal DNA is challenged by intrinsic and exogenous sources of damage. double-strand breaks (DSBs) are extremely dangerous lesions. RAD51 plays a central role homologous recombinational DSB repair, facilitating the recombination damaged with intact eukaryotes. accumulates at sites containing damage to form nuclear foci. However, mechanism accumulation still unclear. Posttranslational modifications proteins, such as phosphorylation, acetylation...
RAD51, an essential eukaryotic DNA recombinase, promotes homologous pairing and strand exchange during recombination the recombinational repair of double breaks. Mutations that up- or down-regulate RAD51 gene expression have been identified in several tumors, suggesting inappropriate activity may cause tumorigenesis. To identify chemical compounds affect activity, present study, we performed RAD51-mediated assay presence 185 compounds. We found 4,4′-diisothiocyanostilbene-2,2′-disulfonic...
The meiosis‐specific synaptonemal complex protein SYCP3 has been reported to be aberrantly expressed in tumours. However, contrast its well‐defined function meiosis, possible role mitotic cells is entirely unknown. Here, we show that a range of primary tumours and it impairs chromosomal integrity cells. Expression inhibits the homologous recombination (HR) pathway mediated by RAD51, inducing hypersensitivity DNA‐damaging agents such as poly(ADP‐ribose) polymerase (PARP) inhibitor...
Mutations and single-nucleotide polymorphisms affecting RAD51 gene function have been identified in several tumors, suggesting that the inappropriate expression of activity may cause tumorigenesis. is an essential enzyme for homologous recombinational repair (HRR) DNA double-strand breaks. In HRR pathway, catalyzes pairing between single-stranded (ssDNA) double-stranded (dsDNA), which central step pathway. To identify a chemical compound regulates homologous-pairing RAD51, present study, we...