A5043350762- Epigenetics and DNA Methylation
- Lymphatic System and Diseases
- Diagnosis and Treatment of Venous Diseases
- Pluripotent Stem Cells Research
- Virology and Viral Diseases
- CRISPR and Genetic Engineering
- Circadian rhythm and melatonin
- Genomics and Chromatin Dynamics
- Animal Virus Infections Studies
- Adipose Tissue and Metabolism
- Reconstructive Surgery and Microvascular Techniques
- Organ and Tissue Transplantation Research
- Photoreceptor and optogenetics research
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Sympathectomy and Hyperhidrosis Treatments
- Lymphatic Disorders and Treatments
- Viral Infections and Immunology Research
- Renal and related cancers
- Protein Degradation and Inhibitors
- Organ Donation and Transplantation
- Planarian Biology and Electrostimulation
- Regulation of Appetite and Obesity
- Dietary Effects on Health
- Nerve injury and regeneration
Tohoku University
2016-2025
RIKEN BioResource Research Center
2024
Otsuka (Japan)
2012-2022
de Duve Institute
2021
UCLouvain
2021
Kansai Rosai Hospital
2018
Japan Agency for Medical Research and Development
2018
The University of Tokyo
2007-2016
Osaka University
2006-2012
Jichi Medical University
2005-2010
Introduction To date, an electron microscopy study of the collecting lymphatic vessels has not been conducted to examine early stages lymphedema. However, such histological studies could be useful for elucidating mechanism lymphedema onset. The aim this was clarify changes occurring in after lymphadenectomy. Methods on 114 specimens from 37 patients who developed lower limbs receiving surgical treatment gynecologic cancers and consulted University Tokyo Hospital affiliated hospitals April...
Various peripheral tissues show circadian rhythmicity, which is generated at the cellular level by their own core oscillators that are composed of transcriptional/translational feedback loops involving a set clock genes. Although circulating levels some adipocytokines, i.e. bioactive substances secreted adipocytes, on 24-h rhythmic cycle, it remains to be elucidated whether gene system works in adipose tissue. To address this issue, we investigated daily mRNA expression profiles genes and...
There is an urgent need for new, potent antituberculosis (anti-TB) drugs with novel mechanisms of action that can be included in new regimens to shorten the treatment period TB. After screening a library carbostyrils, we optimized 3,4-dihydrocarbostyril derivatives and identified OPC-167832 as having activity. The MICs compound
Significance Primordial germ cells (PGCs) are the origin of and critically important for continuity multicellular organisms. Although unique characteristics mouse PGCs have been described in gene expression epigenome levels, metabolomic proteomic profiles their significance PGC properties unclear. Our findings this study demonstrate not only distinct energy metabolisms pluripotent stem (PSCs), but also essential contribution enhanced oxidative phosphorylation glycolysis specification from...
Recent studies have suggested that the impairment of circadian molecular clock in peripheral tissues, including adipose tissue, is involved development metabolic syndrome. Although disorder often caused by dietary obesity, it remains to be elucidated whether obesity or high‐caloric intake per se affects system. To address this issue, study investigated effect high‐fat feeding on rhythmic mRNA expression genes (Clock, Bmal1, Per1, Per2, Cry1, Cry2, and Dbp) mouse visceral tissue liver. Mice...
Recent studies have shown the gene expression of several transporters to be circadian rhythmic. However, it remains elucidated whether P-glycoprotein, which is involved in transport many medications, undergoes 24 h rhythmicity. To address this issue, we investigated daily profiles P-glycoprotein mRNA and protein levels peripheral mouse tissues. In liver intestine, but not kidney, Abcb1a showed clear On other hand, Abcb1b Abcb4, genes, did exhibit significant rhythmic studied whole also...
Uterine transplantation experiments have been performed in various animal species for future clinical applications of uterine permanent factor infertility humans. The aim this study was to confirm the feasibility auto-transplantation cynomolgus monkeys by developing new surgical techniques.Two female underwent surgery under general anesthesia. uterus with vascular grafts and vaginal wall removed, back-table preparation using heparinized saline. anastomosed stump blood vessels pelvis,...
We have described a procedure to minimize surgical wounds, in which lymph vessels and skin venules are identified by indocyanine green (ICG) lymphography the AV300 noncontact visualization system (AccuVein, Cold Spring Harbor, NY), respectively. This approach allows accurate decisions regarding sites of incision for lymphatic venous anastomosis (LVA). method was applied patient with right upper-limb lymphedema after breast cancer therapy. The low-invasive can be used before during surgery....
BACKGROUNDUterus transplantation may be the only theoretical option for some women, example, those with congenital uterine infertility or who have undergone hysterectomy. In this study, we evaluated intra- and post-operative blood flow conditions of vascular anastomosed regions blood-perfused area transplanted uterus in a cynomolgus macaque model autotransplantation.
Significance Successful microbial infections typically involve the subversion of host immune pathways by pathogen-specific virulence factors. Here, we uncovered that viruses and bacteria independently evolved effectors targeting same conserved loop mitogen-activated protein kinase (MAPK) p90-ribosomal S6-kinases (RSKs). Kinase usurpation relies on a previously unidentified short linear motif (SLiM) shared these Mechanistically, RSK binding prevents its dephosphorylation, thus promoting...
Physical stimulation, which is a key factor affecting the metabolism of osteoblasts and their precursor cells, plays an important role in bone remodeling; however, micro-vibrations osteoblast differentiation unclear. In present study, we determined effects frequency-regulated repeated micro-vibration (FRMV) on cell proliferation established method to induce through FRMV using mouse pre-osteoblast-like line MC3T3-E1, widely used research. The results indicated that significantly influenced...
Borna disease virus (BDV) is a nonsegmented, negative-strand RNA that employs several unique strategies for gene expression. The shortest transcript of BDV, X/P mRNA, encodes at least three open reading frames (ORFs): upstream ORF (uORF), X, and P in the 5' to 3' direction. X negative regulator viral polymerase activity, while phosphoprotein necessary cofactor complex, suggesting translation controlled rigorously, depending on replication. However, mechanism used by polycistronic mRNA has...
Abstract We previously reported that sugar levels in the silkworm hemolymph, i.e., blood, increase immediately (within 1 h) after intake of a high-glucose diet and administration human insulin decreases elevated hemolymph silkworms. In this hyperglycemic model, however, pioglitazone or metformin, drugs used clinically for treatment type II diabetes, have no effect. Therefore, here we established model diabetes evaluation anti-diabetic such as metformin. Silkworms fed over long time-period...
Secondary lymphedema is defined as swelling of the limbs caused by retention lymph after cancer therapy. We diagnosed using indocyanine green (ICG) fluorescent lymphography and developed a classification based on 12 regional types edema in lower bodies, with goal improved understanding pathology.The subjects were 72 consecutive female patients aged 25 to 88 years (mean, 54.5 years) secondary extremities abdominal area. The traditional diagnosis was stages 0, 1, 2, 3 4 5, 11, 19, 24, 13...
Abstract Lymphatico‐venous anastomosis (LVA) is used to resolve lymph retention in lymphedema. However, the postoperative outcome of lower limb lymphedema poorer than that for upper lymphedema, because location heart level. Improvement therapeutic requires application as many anastomoses possible a limited operation time, particularly since there positive correlation between number and effect LVA. In this case, we described method increase efficiency lymphatico‐venous bilateral severe...
3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase, a mevalonate synthetase, is required for the growth of Staphylococcus aureus. However, essential role enzyme in cell has remained unclear. Here we show that three mutants possessed single-base substitutions mvaA gene, which encodes HMG-CoA temperature-sensitive phenotype. The phenotype was suppressed by addition or farnesyl diphosphate, product synthesized from mevalonate. Farnesyl diphosphate precursor undecaprenyl phosphate peptidoglycan...