A5103029319- Organ and Tissue Transplantation Research
- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- Growth Hormone and Insulin-like Growth Factors
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Endometriosis Research and Treatment
- Hypothalamic control of reproductive hormones
- Gestational Diabetes Research and Management
- Organ Donation and Transplantation
- Endometrial and Cervical Cancer Treatments
- Estrogen and related hormone effects
- Molecular Biology Techniques and Applications
- Ovarian cancer diagnosis and treatment
- Pregnancy and Medication Impact
- Preterm Birth and Chorioamnionitis
- Reproductive Physiology in Livestock
- Birth, Development, and Health
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Reconstructive Surgery and Microvascular Techniques
- Bariatric Surgery and Outcomes
- Dermatologic Treatments and Research
- Gynecological conditions and treatments
- Metabolism and Genetic Disorders
- Maternal Mental Health During Pregnancy and Postpartum
Kyoto University
2006-2020
Yokohama City University Medical Center
2019
Kanagawa Prefectural Hospital Organization
2019
Yokohama City University
2019
Yokohama Rosai Hospital
2019
Kanagawa Cancer Center
2019
Chigasaki Rehabilitation College
2019
Tokyo Medical University Hospital
2019
Japanese Red Cross Nagoya Daiichi Hospital
2011
University of Tokyo Health Sciences
2011
To analyze the structure of LH in three patients with immunologically anomalous LH, whole coding region beta-subunit gene was examined. These were infertile, and their serum levels could not be measured an immunoassay kit. Immunoblotting showed no marked changes molecular size beta. Genomic DNA extracted from peripheral lymphocytes normal controls, beta genes amplified by polymerase chain reaction technique, using primer pairs that are capable specifically amplifying only without...
For women with congenital uterine infertility, or for those who have undergone hysterectomy, transplantation is one of the potential treatments to regain fertility. In this study, we utilized a primate model transplantation, and evaluated patency our microsurgical anastomoses, perfusion transplanted uterus. Two female cynomolgus monkeys underwent surgery. We anastomosed two arteries vein in Case 1 veins 2. The used were anastomosis was done external iliac artery. ovarian both animals, but...
Striae gravidarum is a physiological skin change that many pregnant women experience during pregnancy. The striae are often accompanied by reddish purple color pregnancy, and then lose pigmentation become atrophic in the long term after seems to be undesirable women. However, impact of on who it has not been clarified. aim this study was evaluate generic dermatology-specific quality life (QOL) A cross-sectional conducted at three private clinics typical urban area Japan. We recruited 447 36...
There have been few studies concerning the clinical pathology of squamous cell carcinoma arising from ovarian mature cystic teratoma. Thus, objective this study is to determine clinicopathologic factors affecting survival in rare tumor.From September 1979 June 1996, 37 patients with teratoma were treated by Tokai Ovarian Tumor Study Group. A retrospective and analysis these was performed. The mode infiltration classified into expansive, moderately diffused, diffused patterns.Although 5-year...
Abstract Aim Uterine transplantation ( UTx ) is a potential option for child‐bearing in women with uterine infertility. Recovery of function after allogeneic non‐human primates has not been reported. The objective this study to establish the functional transplant model primates. Methods Uteri two cynomolgus monkeys were simultaneously removed, cooled at 4° C and perfused heparin saline. uteri interchanged each other then orthotopically transplanted. Immunosuppressive protocols included use...
ADVERTISEMENT RETURN TO ISSUEPREVArticlePatterns of alternative splicing fibronectin pre-mRNA in human adult and fetal tissuesFumitaka Oyama, Yoshiharu Murata, Nobuhiko Suganuma, Toshio Kimura, Koiti Titani, Kiyotoshi SekiguchiCite this: Biochemistry 1989, 28, 3, 1428–1434Publication Date (Print):February 7, 1989Publication History Published online1 May 2002Published inissue 7 February 1989https://pubs.acs.org/doi/10.1021/bi00429a072https://doi.org/10.1021/bi00429a072research-articleACS...
BACKGROUNDUterus transplantation may be the only theoretical option for some women, example, those with congenital uterine infertility or who have undergone hysterectomy. In this study, we evaluated intra- and post-operative blood flow conditions of vascular anastomosed regions blood-perfused area transplanted uterus in a cynomolgus macaque model autotransplantation.
To study the aging process in human ovary, we analyzed accumulation of mitochondrial DNA (mtDNA) mutation ovary women various ages. Thirty-nine tissues from ovaries and abdominal muscles, ranging those a fetus to 77-yr-old woman, were collected at artificial abortion or gynecological surgery. Total was extracted these tissues, parts mtDNA amplified via polymerase chain reaction. Through experiments, found 4977-bp deletion between ATPase8 ND5 genes ovarian samples obtained menopausal...
To clarify whether apoptosis is involved in endometriosis, we obtained eutopic endometrial tissues along with endometriotic from the uterus (adenomyosis) (n = 12) and ovary patients undergoing gynaecological surgery. Apoptosis-induced DNA fragmentation was detected by TdT-mediated dUTP-biotin nick-end labelling method, immunostaining a monoclonal antibody against Fas, Ley or B-cell leukaemia/lymphoma-2 (bcl-2) also performed using same tissue section. Analysis showed that occurring all...
Background Uterine blood flow is an important factor in uterine viability, but the number of vessels required to maintain viability uncertain. In this study, indocyanine green (ICG) fluorescence imaging was used examine hemodynamics and associated with cynomolgus macaque. Methods The uterus a female macaque cut from vaginal canal mimic situation during trachelectomy or transplantation surgery which perfusion maintained only ovarian vessels. Intraoperative observed using ICG under conditions...
The mutations (Trp8 —> Arg and Ile15 Thr) in the human LHbeta -subunit caused by nucleotide point gene were reported women with immunologically anomalous LH menstrual disorders. To estimate effects of on bioactivity vitro vivo, we constructed a containing this mutation each site or both sites site- directed mutagenesis analyzed bioactivities mutant expressed Chinese hamster ovary cells. Although no alterations noted receptor-binding activity due to mutations, LHs at Trp8 beta-subunit showed...
Abstract Previous kinetic studies have characterized the intracellular folding pathway of human chorionic gonadotropin (hCG)-beta subunit in which each intermediates can be biochemically identified based on formation disulfide (S-S) bonds: p beta 1-early--> 1-late--> 2-free--> 2-combined--> native hCG-alpha beta. Based these data, we postulated that hCG-beta coincides with specific S-S bonds. We now tested this hypothesis employing Chinese hamster ovary cells transfected mutated genes Cys...
Abstract Aim This study evaluated the usefulness of daily walking for gestational diabetes mellitus (GDM) management by analyzing relationship between and glucose tolerance in pregnant women with GDM who were second trimester. Methods longitudinal was conducted at TOYOTA Memorial Hospital Toyota, Japan, from January 2015 to June 2016. Pregnant wore accelerometers on waist 7–12 weeks. Results Seventy‐three included present study; data collected 24 analyzed. The estimated number steps walked...
Background. An association of human leukocyte antigen (HLA)-DQw3 alleles with squamous cell carcinoma (SCC) the cervix has been reported in some European populations, but significance HLA-DQw3 not examined other populations to authors' knowledge. The interaction between and papillomavirus (HPV) SCC remains be clarified. Methods. To elucidate HLA-DQ cervix, DNA samples extracted from blood lymphocytes 23 patients were amplified by polymerase chain reaction (PCR) using specific primers for...
Since the incidence of mucinous carcinoma ovary is relatively low, with only small numbers cases at any institution, detailed clinicopathologic studies on prognosis and care patients are missing. Forty-four were histopathologically subclassified into endocervical (n = 8) intestinal types 36), studied for clinical manifestations. All tumors type stage I, whereas 14 intestinal-type II or higher (p < 0.05). Stromal invasion was not observed in 44 tumors, 13 which I. Analysis prognostic factors...
To analyze the effects of treatments with GH and cyclic estrogen/progesterone (E/P) replacement on bone mineralization in patients Turner’s syndrome (TS), mineral density (BMD) was measured longitudinally. BMDs whole body lumbar spine 16 adult female TS (17-38 year old; 0-20 years by length E/P treatment) were assessed using dual energy X-ray absorptiometry one to 5 times over a treatment period up 7 maximum. performed 9 cases (GH group), but not remaining (non-GH group). therapy initiated...
The effect of estrogen on prolactin (PRL) synthesis at a single-cell level was studied by in situ hybridization and immunohistochemistry. Long-term treatment increased PRL-containing cells from 10–20% total cell population to 80–90%, as revealed PRL mRNA containing also similar fashion. Moreover, cytoplasmic expressed the number silver grains per 4- 5-fold estrogen. These results suggest that long-term causes not only proliferation but an increase single cell.
There are six intramolecular disulfide (S-S) bonds that form during intracellular folding of the human chorionic gonadotropin (hCG)-beta subunit. Site-directed mutagenesis every pair Cys residues involved in formation each S-S bond was used to examine roles play beta subunit and secretion. Tryptic maps secreted hCG-beta showed only one formed all mutants failed fold from earliest detectable intermediate, p 1, into a second major 2 (C34A-C88A, C38A-C57A or C9A-C90A mutants), whereas 5...