Liposuction aspirates (primarily saline solution, blood, and adipose tissue fragments) separate into fatty fluid portions. Cells isolated from the portion are termed processed lipoaspirate (PLA) cells contain adipose-derived adherent stromal (ASCs). Here we define of liposuction as aspirate (LAF) cells. Stromal vascular fractions (SVF) were separately both portions characterized under cultured non-cultured conditions. A comparable number LAF PLA freshly isolated, but fewer adherent. CD34+...

The anterolateral thigh flap is a septocutaneous artery based on the or muscle perforators of lateral circumflex femoral system. Little has been reported about variations in its vascular anatomy and application for head neck reconstruction. We report 22 cases which this was used reconstruction defects. Based our clinical cadevaric experiences, derivation pedicle four by are derived from descending branch system and/or transverse that system, there no but originating Clinically, locations can...

Over the last eight years, authors analyzed obstructive lymphedema of a unilateral upper extremity in total 27 females, comparing use supramicrosurgical lymphaticovenule anastomoses and/or conservative treatment. The most common cause edema was mastectomy, with or without subsequent radiation therapy for breast cancer. As an objective assessment extent edema, circumferences affected and opposite normal forearms were measured at 10 cm below olecranon arm. Twelve these patients received...

Background: Indocyanine green lymphography has been a highly useful modality in the clinical examination and surgical management of patients with lymphedema. No formal classification system indocyanine imaging findings according to severity lymphedema exists, however. The purpose this study was describe, analyze, classify characteristic uniformly guide using modality. Methods: Forty-five (78 limbs) lower extremity underwent lymphography. All images were recorded photographs videos. Images...

Management of arm lymphedema following breast cancer treatment is challenging, and emphasis should be put on early diagnosis prevention secondary lymphedema. Indocyanine green lymphography becoming a method choice for evaluation lymphedema.Twenty patients with after underwent indocyanine lymphography. Characteristic findings were analyzed according to corresponding clinical stages duration edema. Based changes in progression lymphedema, new severity stage, dermal backflow was developed...

Introduction To date, an electron microscopy study of the collecting lymphatic vessels has not been conducted to examine early stages lymphedema. However, such histological studies could be useful for elucidating mechanism lymphedema onset. The aim this was clarify changes occurring in after lymphadenectomy. Methods on 114 specimens from 37 patients who developed lower limbs receiving surgical treatment gynecologic cancers and consulted University Tokyo Hospital affiliated hospitals April...

Background: Early diagnosis and treatment are as important for management of secondary lymphedema following cancer in primary treatment. Indocyanine green lymphography is the modality choice routine follow-up evaluation patients at high risk developing after therapy. Methods: Fifty-six limbs 28 so-called unilateral lower extremity who underwent indocyanine were compared with dermal backflow patterns on asymptomatic assessed using leg stage. Results: Of patients, detected 19 but absent nine...

Background Secondary lymphedema causes swelling in limbs due to lymph retention following node dissection cancer therapy. Initiation of treatment soon after appearance edema is very important, but there no method for early diagnosis lymphedema. In this study, we compared the utility four diagnostic imaging methods: magnetic resonance (MRI), computed tomography (CT), lymphoscintigraphy, and Indocyanine Green (ICG) lymphography. Patients Methods Between April 2010 November 2011, examined 21...

A gluteal perforator-based flap employing the gluteus maximus muscle perforators located around sacrum is described. cadaveric study disclosed existence of several significant all region. Among these, parasacral originating from internal pudendal artery and lateral sacral have proven useful for repair pressure sores. total eight decubitus in seven patients were treated with flaps. There no postoperative complications, such as necrosis wound infection, exception fistula formation one case....

Due to its increasing popularity, more and articles on the use of perforator flaps have been reported in literature during past few years. Because area is new rapidly evolving, there are no definitions standard rules terminology nomenclature, which creates confusion when surgeons try communicate compare surgical techniques. This article attempts represent opinion a group pioneers field flap surgery. consensus was reached after meeting held Fifth International Course Perforator Flaps Gent,...

Chimeric composite flaps combined using microanastomoses consist of two or more tissues, each with an isolated pedicle and a single vascular source. Free chimeric the lateral circumflex femoral system were used to treat massive defects head neck in 10 cases. A anterolateral thigh flap vascularized iliac bone graft based on deep was most commonly combination. An anteromedial paraumbilical perforator-based also this principal The advantages over other osteocutaneous are: (1) is relatively thin...

The superficial circumflex iliac artery perforator (SCIP) flap differs from the established groin in that it is nourished by only a of system and has short segment (3 to 4 cm length) this vascular system. Three cases which free flaps were successfully transferred for coverage soft-tissue defects limb are described article. advantages as follows: no need deeper longer dissection pedicle vessel, shorter elevation time, possible thinning with primary defatting, possibility an adiposal...

Background: Over the past few years, use of propeller flaps, which base their blood supply on subcutaneous tissue or isolated perforators, has become increasingly popular. Because no consensus yet been reached terminology and nomenclature flap, different confusing uses term can be found in literature. Methods: In this article, authors report definition classification flaps by that gathered at First Tokyo Meeting Perforator Propeller Flaps June 2009. Some peculiar aspects surgical technique...

Abstract To seek a suitable scaffold for cartilage tissue engineering, we compared various hydrogel materials originating from animals, plants, or synthetic peptides. Human auricular chondrocytes were embedded in atelopeptide collagen, alginate, PuraMatrix™, all of which are will soon be clinically available. The the collagen proliferated well, while others showed no proliferation. A high‐cell density culture within each enhanced expression type II mRNA, when with that without hydrogel. By...

A new method for easy detection of functional lymphatic vessels in the superficial layer is reported. In a clinical trial, lymphography using indocyanine green dye near-infrared fluorescence labeling lymphaticovenular anastomoses was performed 5 patients with lymphedema. The technique simple and enables minimally invasive operation to be performed. results indicate that this useful acceptance as one examinations evaluation

Over the last 9 years, authors analyzed lymphedema of lower extremity in a total 25 patients, comparing use supermicrosurgical lymphaticovenular anastomosis and/or conservative treatment. The most common cause edema was hysterectomy, with or without subsequent radiation therapy for uterine cancer. Among 12 cases that underwent only treatment, one case showed decrease over 4 cm circumference leg. average period treatment 1.5 and decreased 0.6 (8 percent preoperative excess). Thirteen patients...

Among various surgical treatments, lymphaticovenular anastomosis (LVA), which bypasses congested lymph into venous circulation, is the least invasive treatment. However, it usually entails skin incisions of around 3 cm, and operation time 4 hours. With multiple supermicrosurgeons under guidance indocyanine green lymphography, LVAs can be simultaneously performed local anesthesia within approximately 2 hours via small with length less than 1 allowing minimally lymphatic supermicrosurgery...

Lymphedema is known to be caused by many pathologic conditions; however, its diagnostic and therapeutic strategies remain unestablished. In this study, we investigated the usefulness of a novel lymphographic method based on fluorometric sensing using indocyanine green (ICG) dye for imaging lymphatic vessels rat models. The real-time system enabled visualization superficial with diameter 0.1 mm in 33 frames/second. addition, morphologic changes radiation-induced lymphedema model were detected...

Background: Lymphaticovenular anastomosis has become an increasingly common treatment for lymphedema. Supermicrosurgical techniques are essential the successful performance of lymphaticovenular anastomosis. A positive correlation between number anastomoses performed and therapeutic efficacy been reported, in performing these anastomoses, establishment as many bypasses possible is important. Methods: Forty limbs 20 patients with lower extremity lymphedema who underwent our department were...

Measurement of the circumference is most commonly employed method for evaluating extremity lymphedema. However, comparison between different patients difficult with this measurement. To resolve problem, we have formulated a new index, upper lymphedema (UEL) which can be easily obtained from measurements body. We evaluated correlation UEL index and clinical stage in UEL. The indices were significantly correlated stages could used as severity scale. lower makes objective assessment through...

Background: The authors investigated the relationship between findings from indocyanine green lymphography and condition of lymphatics according to Normal, Ectasis, Contraction, Sclerosis Type classifications observed in each area during surgery. Methods: examined 43 limbs 25 patients who had undergone lymphaticovenous anastomosis April July 2012 at Department Plastic Reconstructive Surgery, University Tokyo Hospital. After injection green, linear, splash, stardust, diffuse patterns were...