A5048462960- Hydrogels: synthesis, properties, applications
- Osteoarthritis Treatment and Mechanisms
- Bone Metabolism and Diseases
- Cancer, Hypoxia, and Metabolism
- Bone Tissue Engineering Materials
- Bone health and treatments
- RNA modifications and cancer
- Inflammatory mediators and NSAID effects
- 3D Printing in Biomedical Research
- Advanced Materials and Mechanics
- Polymer Nanocomposites and Properties
- Hedgehog Signaling Pathway Studies
- TGF-β signaling in diseases
- Advanced Polymer Synthesis and Characterization
- RNA Research and Splicing
- NF-κB Signaling Pathways
- Dental Implant Techniques and Outcomes
- Advanced Sensor and Energy Harvesting Materials
- Pluripotent Stem Cells Research
- Surfactants and Colloidal Systems
- biodegradable polymer synthesis and properties
- RNA Interference and Gene Delivery
- Periodontal Regeneration and Treatments
- Cytokine Signaling Pathways and Interactions
- Supramolecular Self-Assembly in Materials
The University of Tokyo
2015-2024
Kanagawa University of Human Services
2019-2024
Nagasaki University
2022
Bunkyo University
2017-2021
Skåne University Hospital
2020
Lund University
2020
Graduate School USA
2014-2019
Louisiana State University Health Sciences Center New Orleans
2018
John Wiley & Sons (United States)
2018
Smith College
2018
As a new class of high-strength hydrogels, we designed tetra-PEG gel by combining two symmetrical tetrahedron-like macromonomers the same size. Because nanostructural unit network was defined length tetrahedral PEG arm, had homogeneous structure and resultant high mechanical strength comparable to that native articular cartilage. Furthermore, since formed mixing biocompatible macromonomer solutions, gelation reaction itself were also biocompatible. The breaking local maxima at overlap...
Based on the fact that aging is associated with a reciprocal decrease of osteogenesis and an increase adipogenesis in bone marrow osteoblasts adipocytes share common progenitor, this study investigated role PPARγ, key regulator adipocyte differentiation, metabolism. Homozygous PPARγ-deficient ES cells failed to differentiate into adipocytes, but spontaneously differentiated osteoblasts, these were restored by reintroduction PPARγ gene. Heterozygous mice exhibited high mass increased...
Based on the fact that aging is associated with a reciprocal decrease of osteogenesis and an increase adipogenesis in bone marrow osteoblasts adipocytes share common progenitor, this study investigated role PPARγ γ, key regulator adipocyte differentiation, metabolism.Homozygous γ-deficient ES cells failed to differentiate into adipocytes, but spontaneously differentiated osteoblasts, these were restored by reintroduction γ gene.Heterozygous mice exhibited high mass increased...
Optimizing Injectable Hydrogels hydrogels are showing promise as scaffolds in regenerative medicine because they can be injected liquid form and transform situ into the gel state. However, when exposed to ionic solutions, such those found body, increase volume by a factor of 2, which weaken material. Kamata et al. (p. 873 ) added thermoresponsive component hydrogel so that would tend collapse shape heated counteract hydrogel's tendency swell. Indeed, resulting retained its unswollen...
Indian hedgehog (Ihh) is indispensable for development of the osteoblast lineage in endochondral skeleton. In order to determine whether Ihh directly required differentiation, we have genetically manipulated smoothened (Smo), which encodes a transmembrane protein that essential transducing all Hedgehog (Hh) signals. Removal Smo from perichondrial cells by Cre-LoxP approach prevents formation normal bone collar and also abolishes primary spongiosa. Analysis chimeric embryos composed wild-type...
Abstract Objective By producing instability in mouse knee joints, we attempted to determine the involvement of runt‐related transcription factor 2 (RUNX‐2), which is required for chondrocyte hypertrophy, development osteoarthritis (OA). Methods An experimental OA model was created by surgical transection medial collateral ligament and resection meniscus joints heterozygous RUNX‐2–deficient (Runx2 +/− ) mice wild‐type littermates. Cartilage destruction osteophyte formation tibial cartilage...
The structure of Tetra-PEG gel, a new class biocompatible, easy-made, and high-strength hydrogel consisting four-arm polyethylene glycol (PEG) network, has been investigated by means small-angle neutron scattering (SANS). Since the gel is prepared cross-end-coupling two kinds PEG macromers having different functional groups at ends, i.e., amine group succinimidyl ester respectively, coupling reaction occurs exclusively between chains carrying groups. SANS results showed that macromer aqueous...
Exposure of articular cartilage to excessive mechanical loading is deeply involved in the pathogenesis osteoarthritis. Here, we identify gremlin-1 as a loading-inducible factor chondrocytes, detected at high levels middle and deep layers after cyclic strain or hydrostatic pressure loading. Gremlin-1 activates nuclear factor-κB signalling, leading subsequent induction catabolic enzymes. In mice intra-articular administration antibody chondrocyte-specific deletion decelerates osteoarthritis...
. To evaluate the validity and reliability of Oura Ring Generation 3 (Gen3) with Sleep Staging Algorithm 2.0 (OSSA 2.0) through multi-night polysomnography (PSG). Participants were 96 generally healthy Japanese men women aged between 20 70 years contributing 421,045 30-s epochs. scoring was performed according to American Academy Medicine criteria. Each participant could contribute a maximum three (PSG) nights. Within-participant means created for each sleep measure paired t-tests used...
Abstract Objective To regenerate permanent cartilage, it is crucial to know not only the necessary conditions for chondrogenesis, but also sufficient conditions. The objective of this study was determine signal chondrogenesis. Methods Embryonic stem cells that had been engineered fluoresce upon chondrocyte differentiation were treated with combinations factors and detected as fluorescence. We screened combination could induce fluorescence within 3 days. Then, primary mesenchymal cells,...
In developing murine growth plates, chondrocytes near the articular surface (periarticular chondrocytes) proliferate, differentiate into flat column-forming proliferating cells (columnar chondrocytes), stop dividing and finally hypertrophic cells. Indian hedgehog (Ihh), which is predominantly expressed in prehypertrophic cells, stimulates expression of parathyroid hormone (PTH)-related peptide (PTHrP) negatively regulates terminal chondrocyte differentiation through PTH/PTHrP receptor (PPR)....
Abstract Since interaction between bone and lipid metabolism has been suggested, this study investigated the regulation of by adiponectin, a representative adipokine, analyzing deficient overexpressing transgenic mice. We initially confirmed that adiponectin its receptors were expressed in osteoblastic osteoclastic cells, indicating can act on not only through an endocrine pathway as hormone secreted from fat tissue, but also autocrine/paracrine pathway. There was no abnormality mass or...
Although bone morphogenetic proteins (BMPs) are clinically useful for regeneration, large amounts required to induce new formation in monkeys and humans. We found recently that heparin stimulates BMP activity vitro (Takada, T., Katagiri, Ifuku, M., Morimura, N., Kobayashi, Hasegawa, K., Ogamo, A., Kamijo, R. (2003) J. Biol. Chem. 278, 43229-43235). In the present study, we examined whether enhances induced by BMPs vivo attempted determine molecular mechanism which using C2C12 myoblasts....
During vertebrate limb development, growth plate chondrocytes undergo temporally and spatially coordinated differentiation that is necessary for proper morphogenesis. Parathyroid hormone-related peptide (PTHrP), its receptor, the PTH/PTHrP Indian hedgehog are implicated in regulation of chondrocyte differentiation, but specific cellular targets these molecules interactions involved have not been defined. Here we generated chimeric mice containing both wild-type receptor (−/−) cells, analyzed...
A series of model networks consisting polyethylene glycol (PEG), tetra-PEG gels, have been prepared and their structure dynamics investigated by small-angle neutron scattering (SANS) static light (SLS). The Tetra-PEG gels were cross-end coupling two types tetra-arm PEG macromers with molecular weights, Mw, (5 to 40) × 103 g/mol. In the SANS regime, factors both as-prepared swollen can be represented Ornstein−Zernike-type functions superimposed single master curves reduced variables, ξq...
In the growth plate of endochondral bones, parathyroid hormone (PTH)-related peptide (PTHrP) regulates rate chondrocyte maturation from prehypertrophic chondrocytes to hypertrophic chondrocytes. Using an antibody specific for Sox9 phosphorylated at serine 181 (S(181)), one two consensus protein kinase A phosphorylation sites Sox9, we showed that addition PTHrP strongly increased SOX9 in COS7 cells transfected with both SOX9- and PTH/PTHrP receptor-expressing vectors. also SOX9-dependent...
Abstract To seek a suitable scaffold for cartilage tissue engineering, we compared various hydrogel materials originating from animals, plants, or synthetic peptides. Human auricular chondrocytes were embedded in atelopeptide collagen, alginate, PuraMatrix™, all of which are will soon be clinically available. The the collagen proliferated well, while others showed no proliferation. A high‐cell density culture within each enhanced expression type II mRNA, when with that without hydrogel. By...
Nonviral gene vectors from synthetic catiomers (polyplexes) are a promising alternative to viral vectors. In particular, many recent efforts have been devoted the construction of biocompatible polyplexes for in vivo nonviral therapy. A approach this regard is use poly(ethylene glycol) (PEG)-based block catiomers, which form nanoscaled core-shell polyplex with PEG palisades. study, series PEG-based different amine functionalities were newly prepared by simple and affordable procedure based on...
The elastic moduli of elastomeric materials are predicted by the affine or phantom junction network models. Although these models often used, we do not know requirement conditions for each model even validity model. validation is difficult because heterogeneity. In this study, tried to evaluate using Tetra-PEG gel, which has extremely homogeneous structure. We performed stretching and tearing tests, first time, observed transition between around overlapping concentration prepolymers.