A5067637165- Osteoarthritis Treatment and Mechanisms
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Inflammatory mediators and NSAID effects
- Wnt/β-catenin signaling in development and cancer
- Immune Response and Inflammation
- Bone Metabolism and Diseases
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Peripheral Nerve Disorders
- interferon and immune responses
- IL-33, ST2, and ILC Pathways
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Education and Social Development in Ukraine
- Nerve Injury and Rehabilitation
- Natural product bioactivities and synthesis
- Hippo pathway signaling and YAP/TAZ
- DNA Repair Mechanisms
- Cytokine Signaling Pathways and Interactions
- Renal and related cancers
- Orthopedic Surgery and Rehabilitation
- Chemokine receptors and signaling
- S100 Proteins and Annexins
Kibi International University
2022
Kyoto University
2013-2021
The University of Tokyo
2014-2019
Meikai University
2019
Graduate School USA
2013
Tokyo Dental College Ichikawa General Hospital
1987
Keio University
1987
Exposure of articular cartilage to excessive mechanical loading is deeply involved in the pathogenesis osteoarthritis. Here, we identify gremlin-1 as a loading-inducible factor chondrocytes, detected at high levels middle and deep layers after cyclic strain or hydrostatic pressure loading. Gremlin-1 activates nuclear factor-κB signalling, leading subsequent induction catabolic enzymes. In mice intra-articular administration antibody chondrocyte-specific deletion decelerates osteoarthritis...
Significance Here we demonstrate that Hes1, an important target of Notch signaling, modulated pathogenesis osteoarthritis by using Col2a1 - Cre ERT ;Hes1 fl/fl mice. Adamts5 and Mmp13 , catabolic enzymes break down cartilage matrix, were induced Hes1. Additionally, microarray analysis ChIP-seq revealed novel Hes1 genes, including Il6 Il1rl1 coding a receptor for IL-33. CaMK2δ was activated during development. formed protein complex with switched it from transcriptional repressor to activator...
Abstract In vitro studies have shown that Rela/p65, a key subunit mediating NF-κB signalling, is involved in chondrogenic differentiation, cell survival and catabolic enzyme production. Here, we analyse vivo functions of Rela embryonic limbs adult articular cartilage, find protects chondrocytes from apoptosis through induction anti-apoptotic genes including Pik3r1 . During skeletal development, homozygous knockout leads to impaired growth enhanced chondrocyte apoptosis, whereas heterozygous...
To identify genes that maintain the homeostasis of adult articular cartilage and regenerate its lesions, we initially compared four types chondrocytes: (AA) versus growth plate (AG) chondrocytes in rats, superficial layer (IS) deep (ID) epiphyseal infant rats. Microarray analyses revealed 40 186 had ≥10-fold higher expression ratios AA/AG IS/ID, respectively, 16 showed both IS/ID ratios. The results were validated by real-time RT-PCR analysis. Among them, Hoxd1, Fgf18, Esm1 expressed more...
Abstract Background Both loss- and gain-of-function of Wnt/β-catenin signaling in chondrocytes result exacerbation osteoarthritis (OA). Here, we examined the activity roles superficial zone (SFZ) articular cartilage. Methods was analyzed using TOPGAL mice. We generated Prg4-Cre ERT2 ;Ctnnb1 fl/fl ;Ctnnb1-ex3 fl/wt mice for gain-of-function, respectively, SFZ. Regulation Prg4 expression by vitro, as were upstream downstream factors SFZ cells. Results activity, determined reporter, high...
Reactive oxygen species (ROS) play critical roles in self-renewal division for various stem cell types. However, it remains unclear how ROS signals are integrated with machinery. Here, we report that the MAPK14/MAPK7/BCL6B pathway creates a positive feedback loop to drive spermatogonial (SSC) via amplification. The activation of MAPK14 induced MAPK7 phosphorylation cultured SSCs, and targeted deletion Mapk14 or Mapk7 resulted significant SSC deficiency after transplantation. this signaling...
Spermatogonial stem cells (SSCs) are maintained in a special microenvironment called niche. However, much is unknown about components that constitute the Here, we report Cdc42 essential for germline niche development. Sertoli cell-specific Cdc42-deficient mice showed normal premeiotic spermatogenesis. germ gradually disappeared during haploid cell formation and few remained mature testes. transplantation experiments revealed significant loss of SSCs Moreover, deficiency downregulated GDNF,...
Spermatogonial stem cells (SSCs) comprise a small population of germ that have self-renewal potential. However, studies on SSCs are hampered by the lack SSC-specific markers. Although cryptorchid operation is often used to obtain an enriched SSC destroying differentiating using high body temperature, in testes different biological characteristics than those normal environment. Therefore, it necessary develop new methods for selection. In this study, we report method isolating from wild-type...
Superficial zone (SFZ) cells, which are morphologically and functionally distinct from chondrocytes in deeper zones, play important roles the maintenance of articular cartilage. Here, we established an easy reliable method for performance laser microdissection (LMD) on cryosections mature rat cartilage using adhesive membrane. We further examined gene expression profiles SFZ zones by performing RNA sequencing (RNA-seq). validated sample collection methods, amplification RNA-seq data...
Abstract Although reactive oxygen species (ROS) are required for spermatogonial stem cell (SSC) self-renewal, they induce DNA damage and harmful to SSCs. However, little is known about how SSCs protect their genome during self-renewal. Here, we report that Ogg1 essential SSC protection against ROS. While cultured exhibited homologous recombination-based double-strand break repair at levels comparable with those in pluripotent cells, were significantly more resistant hydrogen peroxide than...
S100A1 and S100B are induced by the SOX trio transcription factors (SOX9, SOX5, SOX6) in chondrocytes, inhibit their hypertrophic differentiation culture. However, functional roles of during vivo skeletal development yet to be determined. Here we show that mice deficient both S100a1 S100b genes displayed normal growth from embryonic stage adulthood. Although no compensatory upregulation other S100 family members was observed S100a1/S100b double mutants, related S100a2, S100a4, S100a10,...
Rac1b is frequently expressed in a number of human cancer cells. It still unclear, however, whether causes morphological abnormalities epithelial tissues. To investigate induces changes 3-dimensional structures, we utilized an auxin-dependent protein expression system, which enabled us to rapidly induce and evaluate function MDCK (Madin-Darby Canine Kidney) cysts, model for polarized structure. Cells carrying the wild-type Rac1, constitutively active Rac1V12 gene were morphologically...
Objective Although mouse osteoarthritis (OA) models are widely used, their histological analysis may be susceptible to arbitrariness and inter-examiner variability in conventional methods. Therefore, a method for the unbiased scoring of OA histology is needed. In this study, as first step establishing system, we developed computer-vision algorithm that automatically detects medial lateral compartments knee sections rigorous manner. Design A total 706 images coronal joints stained by...