A5063673622- Robotic Path Planning Algorithms
- Robotics and Automated Systems
- Osteoarthritis Treatment and Mechanisms
- Robot Manipulation and Learning
- Bone Metabolism and Diseases
- Microtubule and mitosis dynamics
- AI-based Problem Solving and Planning
- Pancreatitis Pathology and Treatment
- Fungal and yeast genetics research
- Spinal Fractures and Fixation Techniques
- Multimodal Machine Learning Applications
- Inflammatory mediators and NSAID effects
- Spine and Intervertebral Disc Pathology
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Traffic control and management
- Advanced Graph Neural Networks
- Abdominal vascular conditions and treatments
- Social Robot Interaction and HRI
- Bone health and treatments
- Human-Automation Interaction and Safety
- Cervical and Thoracic Myelopathy
- Kruppel-like factors research
- Pelvic and Acetabular Injuries
- Transportation Planning and Optimization
Keio University
2018-2024
Mitsui Memorial Hospital
2016-2024
Osaka Hospital
2016-2017
Japan Community Healthcare Organization
2017
Okinawa Institute of Science and Technology Graduate University
2013
Kyoto University
2000-2010
Japan Science and Technology Agency
2009
The University of Tokyo
2006-2009
Kyorin University
2004-2008
Kure Kyosai Hospital
2008
Abstract Objective By producing instability in mouse knee joints, we attempted to determine the involvement of runt‐related transcription factor 2 (RUNX‐2), which is required for chondrocyte hypertrophy, development osteoarthritis (OA). Methods An experimental OA model was created by surgical transection medial collateral ligament and resection meniscus joints heterozygous RUNX‐2–deficient (Runx2 +/− ) mice wild‐type littermates. Cartilage destruction osteophyte formation tibial cartilage...
Cohesin complex acts in the formation and maintenance of sister chromatid cohesion during after S phase. Budding yeast Scc1p/Mcd1p, an essential subunit, is cleaved dissociates from chromosomes anaphase, leading to separation. Most cohesin higher eukaryotes, contrast, dissociated well before anaphase. The universal role anaphase thus remains be determined. We report here initial characterization four putative subunits, Psm1, Psm3, Rad21, Psc3, fission yeast. They are for cohesion....
Bone mass and turnover are maintained by the coordinated balance between bone formation osteoblasts resorption osteoclasts, under regulation of many systemic local factors. Phosphoinositide-dependent serine-threonine protein kinase Akt is one key players in signaling potent anabolic This study initially showed that disruption Akt1, a major mice led to low-turnover osteopenia through dysfunctions both cells. Ex vivo cell culture analyses revealed osteoblast dysfunction was traced increased...
The effectiveness and safety of yokukansan (TJ-54), a traditional Japanese medicine (kampo) for the treatment behavioural psychological symptoms dementia (BPSD), were evaluated in 106 patients diagnosed as having Alzheimer's disease (AD) (including mixed-type dementia) or with Lewy bodies. Patients randomly assigned to group A (TJ-54 period I no II; each lasting 4 wk) B (no TJ-54 II). BPSD cognitive functions using Neuropsychiatric Inventory (NPI) Mini-Mental State Examination (MMSE),...
Sister chromatid cohesion is essential for cell viability.We have isolated a novel temperature-sensitive lethal mutant named eso1-H17 that displays spindle assembly checkpoint-dependent mitotic delay and abnormal chromosome segregation.At the permissive temperature, shows mild sensitivity to UV irradiation DNA-damaging chemicals.At nonpermissive arrested in M phase with viability loss due failure establish sister during S phase.The M-phase arrest phenotype, however, suppressed by...
Background Although transition from proliferation to hypertrophic differentiation of chondrocytes is a crucial step for endochondral ossification in physiological skeletal growth and pathological disorders like osteoarthritis, the underlying mechanism remains an enigma. This study investigated role transcription factor CCAAT/enhancer-binding protein β (C/EBPβ) during ossification. Methodology/Principal Findings Mouse embryos with homozygous deficiency C/EBPβ (C/EBPβ−/−) exhibited dwarfism...
cGMP-dependent protein kinase II (cGKII; encoded by PRKG2) is a serine/threonine that critical for skeletal growth in mammals; mice, cGKII deficiency results dwarfism. Using radiographic analysis, we determined this defect was consequence of an elongated plate and impaired chondrocyte hypertrophy. To investigate the mechanism cGKII-mediated hypertrophy, performed substrate array identified glycogen synthase kinase–3β (GSK-3β; Gsk3b) as principal phosphorylation target cGKII. In cultured...
Calcium/calmodulin-dependent protein kinase (CaMK) is required for diverse cellular functions, and similar kinases exist in fungi. Although mammalian CaMK (CaMKK) activates also evolutionarily-conserved AMP-activated (AMPK), CaMKK yet to be established yeast. We here report that the fission yeast Schizosaccharomyces pombe Ssp1 kinase, which controls G2/M transition response stress, putative CaMKK. has a CaM binding domain (CBD) associates with 14-3-3 proteins as does. Temperature-sensitive...
In this study, we examined the mutational spectrum of K-ras in cases gallbladder and carcinoma with an anomalous junction pancreaticobiliary duct (AJPBD).We 35 gallbladders AJPBD (20 hyperplasia, 15 carcinoma) 38 without (four normal gallbladders, four six adenoma, 24 carcinoma). Polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) direct sequencing were performed to detect mutations codon 12 or 13 K-ras.In AJPBD, prevalences mutation 15% (3/20) 60% (6/10) stage I...
Body cells in multicellular organisms are the G0 state, which arrested and terminally differentiated. To understand how state is maintained, genes that specifically expressed or repressed must be identified, as they control G0. In fission yeast Schizosaccharomyces pombe , haploid completely under nitrogen source starvation with high viability. We examined global transcriptome of on course to resume vegetative growth. Approximately 20% transcripts ~5000 increased decreased more than fourfold...
Although degradation of cartilage matrix has been suggested to be a rate-limiting step for endochondral ossification during skeletal development, little is known about the transcriptional regulation. This study investigated involvement KLF5 (Krüppel-like factor 5), an Sp/KLF family member, in development. was expressed chondrocytes and osteoblasts but not osteoclasts. The heterozygous deficient (KLF5+/-) mice exhibited growth retardation perinatal period. chondrocyte proliferation...
Retrospective multi-center study.This study was conducted to clarify the incidence and neurological outcomes of fall-related deterioration subjective symptoms in patients undergoing surgical treatment.The evidence that minor trauma, including falls, increases risk worsening cervical myelopathy is insufficient.A retrospective analysis who had undergone surgery for at 12 participating institutes conducted. Patients symptomatic from January 2012 December 2013 completed least 1-year follow-up...
Fission yeast, Schizoaccharomyces pombe, is a model for studying cellular quiescence. Shifting to medium that lacks nitrogen-source induces proliferative cells enter long-term G0 Klf1 Krüppel-like transcription factor with 7-amino acid Cys2His2-type zinc finger motif. The deletion mutant, ∆klf1, normally divides in vegetative medium, but proliferation not restored after Cell biologic, transcriptomic, and metabolomic analyses revealed unique phenotype of the ∆klf1 mutant Mutant had diminished...
AbstractSister chromatid separation requires two steps of proteolysis. Securin, the chaperon and inhibitor separase, is destructed in anaphase after polyubiquitination, resulting activated separase cleaves cohesin subunit Scc1/Rad21. Fission yeast securin/Cut2 separase/Cut1 that form complex are essential for viability a number temperature-sensitive (ts) mutants have been isolated. We here report stresses such as 1.2 M sorbitol, 0.6 KCl 0.1 CaCl2 medium suppress ts phenotypes all cut1 three...
It has been reported that the immunosuppressant rapamycin decreases viability of pancreatic beta cells. In contrast, exendin-4, an analogue glucagon-like peptide-1, found to inhibit cell death and increase mass. We investigated effects exendin-4 on cytotoxic effect in Incubation with 10 nM induced 12 h murine line MIN6 cells Wistar rat islets, but not when coincubated exendin-4. Rapamycin was phosphorylation c-Jun amino-terminal kinase (JNK) p38 30 minutes islets while decreased their...
Morel-Lavallée lesion (MLL) is a posttraumatic closed degloving soft tissue injury, in which the subcutaneous tissues are separated from underlying fascia. Surgical treatment recommended if conservative management fails. The conventional surgical for drainage and debridement. A 51-year-old male patient presented with swelling of right thigh incurred during traffic accident. was diagnosed MLL. MLL successfully treated minimally invasive arthroscopic after failure treatment. chosen because...
This paper describes a domestic service robot (DSR) that fetches everyday objects and carries them to specified destinations according free-form natural language instructions. Given an instruction such as "Move the bottle on left side of plate empty chair," DSR is expected identify chair from multiple candidates in environment carry target object destination. Most existing multimodal understanding methods are impractical terms computational complexity because they require inferences for all...
Study Design: Cross-sectional observational study (consecutive case series). Objectives: The aim of this was to define a criterion for achieving successful decompression lumbar spinal stenosis (LSS) using intraoperative ultrasonography (IOUS) and investigate the pathogenesis redundant nerve roots (RNRs) based on ultrasonographic findings. Methods: A total 100 LSS patients (71 males, 29 females, mean age, 71 ± 8 years) with RNRs were enrolled as subjects in study. IOUS performed evaluate...
Sister chromatid cohesion is essential for cell viability. We have isolated a novel temperature-sensitive lethal mutant namedeso1-H17 that displays spindle assembly checkpoint-dependent mitotic delay and abnormal chromosome segregation. At the permissive temperature, eso1-H17 shows mild sensitivity to UV irradiation DNA-damaging chemicals. nonpermissive arrested in M phase with viability loss due failure establish sister during S phase. The M-phase arrest phenotype, however, suppressed by...