A5100751890- Chemical Synthesis and Analysis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- RNA and protein synthesis mechanisms
- Genomics, phytochemicals, and oxidative stress
- Mitochondrial Function and Pathology
- Bacterial Genetics and Biotechnology
- Liver Disease Diagnosis and Treatment
- Renal and related cancers
- ATP Synthase and ATPases Research
- RNA modifications and cancer
- Hormonal Regulation and Hypertension
- Machine Learning in Bioinformatics
- Signaling Pathways in Disease
- Antimicrobial Peptides and Activities
- Receptor Mechanisms and Signaling
- Genetics and Neurodevelopmental Disorders
- Click Chemistry and Applications
- Psoriasis: Treatment and Pathogenesis
- Dermatological and Skeletal Disorders
- Chronic Kidney Disease and Diabetes
- Advanced Materials and Mechanics
- Nitrogen and Sulfur Effects on Brassica
- Biochemical and Structural Characterization
- Cancer-related gene regulation
Shandong University of Science and Technology
2024
Zhejiang Sci-Tech University
2024
University of Pennsylvania
2023
Ottawa Hospital Research Institute
2022-2023
Ottawa Hospital
2022-2023
University of Regina
2017-2023
Second Military Medical University
2013-2021
Peking University
2013-2021
Shenzhen Bay Laboratory
2021
Peking University Shenzhen Hospital
2015-2018
Abstract The addition of a precisely positioned chiral center in the tether constrained peptide is reported, yielding two separable diastereomers with significantly different helicity, as supported by circular dichroism (CD) and NMR spectroscopy. Single crystal X‐ray diffraction analysis suggests that absolute configuration in‐tether helical form R , which agreement theoretical simulations. relationship between secondary structure short peptides their biochemical/biophysical properties...
Highlights•Rewiring of mitochondrial (mt) protein interaction network in distinct cell states•Dramatic changes site-specific phosphorylation during neuronal differentiation•C20orf24 is a respirasome assembly factor depleted patients deficient CIV•NENF binding with DJ-1/PINK1 promotes neurotrophic activity and survivalSummaryMitochondrial (MP) assemblies undergo alterations neurogenesis, complex process vital brain homeostasis disease. Yet which MP remodel differentiation remains unclear....
Abstract Despite recent advances in colloidal hydrogels, designing general and robust strategies that enable the controlled fabrication of hydrogels with strong mechanical properties still proves challenging. Herein, statistical copolymerization‐induced self‐assembly ( stat ‐PISA) monomers hydrogen bonding ability is developed as a strategy for high stretchability, anti‐swelling, self‐healing abilities. This concept first verified by dispersion copolymerization acrylic acid diacetone...
Hepatic cholesterol overload promotes steatohepatitis. Insufficient understanding of liver stress defense impedes therapy development. Here, we elucidate the role transcription factors, nuclear factor erythroid 2 related factor-1 (NRF1) and -2 (NRF2), in counteracting cholesterol-linked stress. Using a diet that increases storage, expression profiles phenotypes from mice with hepatocyte deficiency NRF1, NRF2, or both are compared controls, chromatin immunoprecipitation sequencing is...
A novel and effective helix-stabilizing strategy by introducing a precisely positioned S = NTs chiral center<italic>via</italic>chloramine-T oxidation is demonstrated.
Renal oncocytomas are rare benign tumors of the kidney and characterized by a deficient complex I (CI) enzyme activity oxidative phosphorylation (OXPHOS) system caused mitochondrial DNA (mtDNA) mutations. Yet, little is known about underlying molecular mechanisms alterations metabolic pathways in this tumor. We compared renal with adjacent matched normal tissues on global scale multi-omics approaches, including whole exome sequencing (WES), proteomics, metabolomics, pathway simulation. The...
Abstract Methylglyoxal (MG) production after myocardial infarction (MI) leads to advanced glycation end‐product formation, adverse remodeling, and loss of cardiac function. The extracellular matrix (ECM) is a main target for MG glycation. This suggests that ECM‐mimicking biomaterial therapies may protect the post‐MI environment by removing MG. In this study, mechanisms which recombinant human collagen type I hydrogel therapy confers cardioprotection are investigated. One‐week post‐MI, mice...
Herein we report the first silver catalyzed fluorinated quinoline synthesis via a cascade alkyne–ketone–amino coupling/addition/condensation process in water.
Thiol-ene reactions have been used in a variety of applications that mostly involve an inter-molecular pathway. Herein, we report facile method to construct thioether-tethered cyclic peptides via intra-molecular thiol-ene reaction. This reaction is efficient, selective, and has good residue compatibility. Short with thioether tethers were constructed longer peptides. synthetic may be useful for constructing bioactive Copyright © 2016 European Peptide Society John Wiley & Sons, Ltd.
Inducing α-helicity through side-chain cross-linking is a strategy that has been pursued to improve peptide conformational rigidity and bio-availability. Here we describe the preparation of small peptides tethered chiral sulfoxide-containing macrocyclic rings. Furthermore, study structure-activity relationships (SARs) disclosed properties with respect ring size, sulfur position, oxidation state, stereochemistry show propensity induce α-helicity. Supporting data include circular dichroism...
Although certain human genetic variants are conspicuously loss of function, decoding the impact many is challenging. Previously, we described a patient with leukemia predisposition syndrome (GATA2 deficiency) germline GATA2 variant that inserts 9 amino acids between 2 zinc fingers (9aa-Ins). Here, conducted mechanistic analyses using genomic technologies and rescue system Gata2 enhancer-mutant hematopoietic progenitor cells to compare how 9aa-Ins function genome-wide. Despite nuclear...
Amyloidosis can involve the gastrointestinal (GI) tract, and deposition present with varied histologic patterns that make recognition challenging. This retrospective observational study aimed to characterize in GI tract evaluate key quality metrics, including discrepant cases, improve provide insight for accurate diagnosis. Sixty-two patients (195 biopsies) amyloid involvement of luminal were reviewed. Amyloid subtyping by mass spectrophotometry was available 59 patients. Immunoglobulin...
Abstract The addition of a precisely positioned chiral center in the tether constrained peptide is reported, yielding two separable diastereomers with significantly different helicity, as supported by circular dichroism (CD) and NMR spectroscopy. Single crystal X‐ray diffraction analysis suggests that absolute configuration in‐tether helical form R , which agreement theoretical simulations. relationship between secondary structure short peptides their biochemical/biophysical properties...
We present a bioinformatics database named Renal Gene Expression Database (RGED), which contains comprehensive gene expression data sets from renal disease research. The web-based interface of RGED allows users to query the profiles in various kidney-related samples, including cell lines, human kidney tissues and murine model kidneys. Researchers can explore certain profiles, relationships between genes interests identify biomarkers or even drug targets diseases. aim this work is provide...
Bacterial transcription factors (TFs) are widely studied in Escherichia coli. Yet it remains unclear how individual genes the underlying pathways of TF machinery operate together during environmental challenge. Here, we address this by applying an unbiased, quantitative synthetic genetic interaction (GI) approach to measure pairwise GIs among all E. coli under auxotrophic (rich medium) and prototrophic (minimal static growth conditions. The resulting differential GI networks reveal...
A sulfilimine chiral center in the tether at i, i + 3 positions of short peptides was systematically studied to elucidate chirality-driven conformational changes. rare and unexpected type III β-turn structure induced by an in-tether center, supported circular dichroism spectroscopy, NMR X-ray crystallography.
The effects of N-methylation on CIH peptides' biophysical properties were systematically studied. N-Methylation at the N terminal NH could help improve cellular uptake with a retained helical conformation. This strategy also be applied to longer peptides their stability and uptake.
The non-immune-suppressive cyclophilin inhibitor CRV431 is a clinical candidate to cure nonalcoholic steatohepatitis (NASH) and has the potential treat liver fibrosis cancer incidence. Herein we report concise chemical semisynthesis of in four steps from commercially available cyclosporine, featuring this flow-chemistry-based methylenation an intermolecular ring-closing metathesis Rh-catalyzed diastereoselective hydrogenation.
The 120 bp origin of replication (ori) for the Mycobacterium plasmid pAL5000 has been shown to comprise binding sites protein RepB as well start site transcription repA and repB genes, encoding proteins RepA RepB. In this work it is demonstrated that a third gene product, Rap, involved in addition previously described proteins. smegmatis cells transformed with replicons carrying rap recover markedly faster upon electroporation than those minimal replicon, which lacks rap. gene, oppositely...