A5067693286- Plant Molecular Biology Research
- Plant Reproductive Biology
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Plant tissue culture and regeneration
- Crystallography and molecular interactions
- Plant Physiology and Cultivation Studies
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Plant Disease Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant and animal studies
- Flowering Plant Growth and Cultivation
- Plant Diversity and Evolution
- Plant Taxonomy and Phylogenetics
- Cytokine Signaling Pathways and Interactions
- Light effects on plants
- Plant Genetic and Mutation Studies
- Cardiac Fibrosis and Remodeling
- DNA and Nucleic Acid Chemistry
- Helicobacter pylori-related gastroenterology studies
- Plant Parasitism and Resistance
- Head and Neck Surgical Oncology
- Genomics and Chromatin Dynamics
Fisheries and Oceans Canada
2024
Monash University
2011-2023
University of Ottawa
2015-2022
University of British Columbia
2022
Yale University
2022
South East Technological University
2022
Institute of Technology Carlow
2022
University of Ulster
2021
Altnagelvin Area Hospital
2021
University Hospital Waterford
2003-2021
The early development of the flower Arabidopsis thaliana is described from initiation until opening bud. morphogenesis, growth rate, and surface structure floral organs were recorded in detail using scanning electron microscopy. Flower has been divided into 12 stages a series landmark events. Stage 1 begins with buttress on flank apical meristem. 2 commences when primordium becomes separate Sepal primordia then arise (stage 3) grow to overlie 4). Petal stamen appear next 5) are soon enclosed...
The first step in flower development is the generation of a floral meristem by inflorescence meristem. We have analyzed how this process affected mutant alleles Arabidopsis gene LEAFY. show that LEAFY interacts with another control gene, APETALA1, to promote transition from cloned and, consistent phenotype, we find RNA expressed strongly young primordia. expression procedes homeotic genes AGAMOUS and APETALA3, which specify organ identify within flower. Furthermore, demonstrate homolog...
We describe the effects of four recessive homeotic mutations that specifically disrupt development flowers in Arabidopsis thaliana. Each affects outcome organ development, but not location primordia. Homeotic transformations observed are as follows. In agamous-1, stamens to petals; apetala2-1, sepals leaves and petals staminoid apetala3-1, carpels; pistillata-1, sepals. addition, two these (ap2-1 pi-1) result loss organs, ag-1 causes cells would ordinarily form gynoecium differentiate a...
ABSTRACT We describe allelic series for three loci, mutations in which result homeotic conversions two adjacent whorls the Arabidopsis thaliana flower. Both structure of mature flower and its development from initial primordium are described by scanning electron microscopy. New at APETALA2 locus, ap2-2, ap2-8 ap2-9, cause outer whorls: sepals to carpels (or leaves) petals stamens. Two new PISTILLATA, pi-2 pi-3, second third whorl organs differentiate incorrectly. Homeotic stamens carpels, a...
The coronavirus disease 2019 (COVID-19) pandemic has affected health and economy worldwide on an unprecedented scale. Patients have diverse clinical outcomes, but those with preexisting cardiovascular disease, hypertension, related conditions incur disproportionately worse outcome. high infectivity of severe acute respiratory syndrome 2 is in part to new mutations the receptor binding domain, acquisition a furin cleavage site S-spike protein. continued viral shedding asymptomatic...
ABSTRACT Mutations in the APETALA1 gene disturb two phases of flower development, meristem specification and floral organ specification. These effects become manifest as a partial conversion flowers into inflorescence shoots disruption sepal petal development. We describe changes an allelic series nine apetala1 mutants show that functions are separable. have also studied interaction between other genes by examining phenotypes multiply mutant plants situ hybridization using probes for several...
Mutants of a new gene, TRANSPARENT TESTA GLABRA2 (TTG2), show disruptions to trichome development and tannin mucilage production in the seed coat. The gene was tagged by endogenous transposon Tag1 shown encode WRKY transcription factor. It is first member this large, plant-specific family known control morphogenesis. functions all other genes revealed date involve responses pathogen attack, mechanical stress, senescence. TTG2 strongly expressed trichomes throughout their development,...
To understand better the role of genes in controlling ovule development, a female-sterile mutant, aintegumenta (ant), was isolated from Arabidopsis. In ovules this integuments do not develop and megasporogenesis is blocked at tetrad stage. As pleiotropic effect, narrower floral organs arise reduced numbers. More complete loss occurs when ant mutant combined with homeotic apetala2, suggesting that two share functions initiating organ development. The ANT gene cloned by transposon tagging,...
ABSTRACT Studies of plants with mutations in the CRABS CLAW gene indicate that it is involved suppressing early radial growth gynoecium and promoting its later elongation. It also required for initiation nectary development. To gain further insight, was cloned by chromosome walking. encodes a putative transcription factor containing zinc finger helix-loop-helix domain. The latter resembles first two helices HMG box, known to bind DNA. At least five other genes Arabidopsis carry same...
Summary A series of mutants Arabidopsis thaliana was selected in which the inflorescence stem elongates but loses ability to produce flower primordia on its flanks. Mutants fell into two classes, further occurrences pin‐formed and mutations at a new locus named pinoid . As well as causing defects, result pleiotropic defects development floral organs, cotyledons leaves. Most changes involve number organs produced rather than their differentiation suggesting that PINOID controls an early...
To help understand the process of carpel morphogenesis, roles three development genes have been partitioned genetically. Mutants CRABS CLAW cause gynoecium to develop into a wider but shorter structure, and two carpels are unfused at apex. second gene, SPATULA, show reduced growth style, stigma, septum, transmitting tract is absent. Double mutants crabs claw spatula with homeotic that ectopic demonstrate SPATULA necessary for, inseparable from, development, their action negatively regulated...
Summary Growth of flowering stems in wild‐type Arabidopsis is indeterminate. Many flowers arise sequentially on the flanks apical meristems a phyllotactic spiral. We have isolated eight recessive mutants gene, terminal flower , which inflorescences become determinate. Flower primordia sooner or later ‘invade’ meristem summit leading to cessation its further growth. Primary usually terminate with several part‐flowers lack pedicels, and normal pedicellate may first. By contrast secondary...
ABSTRACT Studies involving mutants of the gene SPATULA indicate that it promotes growth carpel margins and pollen tract tissues derived from them. We show encodes a new member basic-helix-loop-helix family transcription factors. is expressed in marginal throughout their development confirming its role regulating growth. It also many other where may act redundantly to control growth, including peripheral zone shoot apical meristem, specific within leaves, petals, stamens roots. Expression...
ABSTRACT “From our acquaintance with this abnormal metamorphosis, we are enabled to unveil the secrets that normal metamorphosis conceals from us, and see distinctly what, regular course of development, can only infer.” - J. W. von Goethe (1790)
In 1991, we published a paper in Development that proposed the ABC model of flower development, an early contribution to genetic analysis development plants. this, used series homeotic mutants, and double triple establish predictive organ specification developing flowers. This has served as basis for much subsequent work, especially towards understanding seed plant evolution. Here, discuss several aspects this story, could be longer one. One surprising conclusion is materials methods might...
Abstract Structural organization of organs in multicellular organisms occurs through intricate patterning mechanisms that often involve complex interactions between transcription factors regulatory networks. For example, INDEHISCENT (IND), a basic helix-loop-helix (bHLH) factor, specifies formation the narrow stripes valve margin tissue, where Arabidopsis thaliana fruits open on maturity. Another bHLH SPATULA (SPT), is required for reproductive tissue development from carpel margins...
Despite the success of current therapies for acute myocardial infarction (MI), many patients still develop adverse cardiac remodeling and heart failure. With growing prevalence failure, a new therapy is needed that can prevent support tissue repair. Herein, we report on injectable recombinant human collagen type I (rHCI) III (rHCIII) matrices treating MI. Injecting rHCI or rHCIII in mice during late proliferative phase post-MI restores myocardium's mechanical properties reduces scar size,...
Perianth development is specifically disrupted in mutants of the PETAL LOSS (PTL) gene, particularly petal initiation and orientation. We have cloned PTL show that it encodes a plant-specific trihelix transcription factor, one family previously known only as regulators light-controlled genes. transcripts were detected early-developing flower, four zones between initiating sepals their developing margins. Strong misexpression range tissues universally results inhibition growth, indicating its...
The lily retrotransposon del 1-46 is 9345 base pairs (bp) long. It has long terminal repeats (LTRs) of 2406 bp (left) and 2415 (right), which differ in sequence by 1.4%. Sequences similar to those involved priming DNA synthesis retroviruses occur the internal region. Near left LTR a complementary 18 residues at 3' end methionine initiator tRNA three plant species, run 12 purines occurs close right LTR. One reading frame relatively few stop codons. 1462-codon product from this motifs, N C...