A5066589781- Protein Degradation and Inhibitors
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Chromatin Remodeling and Cancer
- Epigenetics and DNA Methylation
- Click Chemistry and Applications
- Biotin and Related Studies
- Bacterial biofilms and quorum sensing
- Monoclonal and Polyclonal Antibodies Research
- Advanced Proteomics Techniques and Applications
- Vibrio bacteria research studies
- Glycosylation and Glycoproteins Research
- Advanced Cellulose Research Studies
- Nanofabrication and Lithography Techniques
- Biofuel production and bioconversion
- DNA Repair Mechanisms
- Chemical Synthesis and Analysis
- Bacteriophages and microbial interactions
- Cancer Mechanisms and Therapy
- Histone Deacetylase Inhibitors Research
- Enzyme Production and Characterization
- Data Management and Algorithms
- Ubiquitin and proteasome pathways
- Biochemical and Structural Characterization
AstraZeneca (United States)
2023
Princeton University
2016-2022
California Institute of Technology
2010-2015
Pasadena City College
2015
Max Planck Institute for Brain Research
2010
Leibniz Institute for Neurobiology
2010
The azide−alkyne cycloaddition provides a powerful tool for bio-orthogonal labeling of proteins, nucleic acids, glycans, and lipids. In some experiments, e.g., in proteomic studies involving affinity purification mass spectrometry, it is convenient to use cleavable probes that allow release labeled biomolecules under mild conditions. Five biotin are described proteins other via cycloaddition. Subsequent conjugation with metabolically protein, these subject cleavage either 50 mM Na2S2O4, 2%...
Chromatin landscape dictates remodeling Large, multicomponent molecular machines known as mammalian SWI/SNF (mSWI/SNF) chromatin-remodeling complexes play critical roles in governing the architecture of our genomes. These entities bind to chromatin (DNA assembled on proteins) inside nucleus and dictate which regions DNA, thus genes genome, are made accessible. It is that this process happens at right times for cells, because disruptions cause diseases such cancers neurodevelopmental...
An approach to proteomic analysis that combines bioorthogonal noncanonical amino acid tagging (BONCAT) and pulsed stable isotope labeling with acids in cell culture (pSILAC) provides accurate quantitative information about rates of cellular protein synthesis on time scales minutes. The method is capable quantifying 1400 proteins produced by HeLa cells during a 30 min interval, scale inaccessible techniques alone. Potential artifacts quantification can be reduced insignificant levels limiting...
Methods for cell-selective analysis of proteome dynamics will facilitate studies biological processes in multicellular organisms. Here we describe a mutant murine methionyl-tRNA synthetase (designated L274GMmMetRS) that charges the noncanonical amino acid azidonorleucine (Anl) to elongator tRNAMet hamster (CHO), monkey (COS7), and human (HeLa) cell lines. Proteins made cells express can be labeled with Anl, tagged dyes or affinity reagents, enriched on resin identification by mass...
Access to protein substrates homogenously modified by ubiquitin (Ub) is critical for biophysical and biochemical investigations aimed at deconvoluting the myriad biological roles Ub. Current chemical strategies ubiquitylation, however, employ temporary ligation auxiliaries that are removed under harsh denaturing conditions have limited applicability. We report an unprecedented aromatic thiol-mediated N-O bond cleavage its application towards native ubiquitylation with auxiliary...
ADP-ribosylation of nuclear proteins is a critical feature various DNA damage repair pathways. Histones, particularly H3 and H2B, are major targets primarily modified on serine with single ADP-ribose unit following damage. While the overall impact PARP1-dependent poly-ADP-ribosylation heavily investigated, very little known about specific roles histone ADP-ribosylation. Here, we report development an efficient modular semisynthetic route to full-length ADP-ribosylated histones chemically...
Cellulases containing a family 9 catalytic domain and 3c cellulose binding module (CBM3c) are important components of bacterial cellulolytic systems. We measured the temperature dependence activities three homologs: Clostridium cellulolyticum Cel9G, Thermobifida fusca Cel9A, C. thermocellum Cel9I. To directly compare their activities, we constructed six new versions enzymes in which GH9-CBM3c domains were fused to dockerin both with without T. fibronectin type 3 homology (Fn3). studied these...
Bio-orthogonal non-canonical amino acid tagging allows time-resolved proteomic analysis of quorum sensing in<italic>Vibrio harveyi</italic>.
Nucleosomes frequently exist as asymmetric species in native chromatin contexts. Current methods for the traceless generation of these heterotypic substrates are inefficient and/or difficult to implement. Here, we report an application SpyCatcher/SpyTag system a convenient route assemble desymmetrized nucleoprotein complexes. This genetically encoded covalent tethering serves internal chaperone, maintained through assembly process, affording nucleosomes following proteolytic removal tethers....
Bispecific antibodies represent an increasingly large fraction of biologics in therapeutic development due to their expanded scope functional capabilities. Asymmetric monovalent bispecific IgGs (bsIgGs) have the additional advantage maintaining a native antibody-like structure, which can provide favorable pharmacology and pharmacokinetic profiles. The production correctly assembled asymmetric bsIgGs, however, is complex engineering endeavor propensity for non-cognate heavy light chains...
Abstract. Several tools have been proposed to automatically create thematic maps. A common drawback of these is that they rarely provide means for users explore their decision trees. Users may thus maps or get some proposed, but not know why suggestions were made. To address this gap, work introduces 3D4DT, an approach trees as a three-dimensional interactive scene. The 3D4DT uses JSON machine-readable format represent and elements user interface (e.g. radio buttons cubes). preliminary...