Annabelle Anandappa
A5031109181- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Chronic Lymphocytic Leukemia Research
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Genetic factors in colorectal cancer
- Cardiac Structural Anomalies and Repair
- Erythrocyte Function and Pathophysiology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Single-cell and spatial transcriptomics
- Childhood Cancer Survivors' Quality of Life
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Cancer Research and Treatments
- Pancreatic and Hepatic Oncology Research
- Glioma Diagnosis and Treatment
- Blood disorders and treatments
University of Cincinnati
2023-2024
Euroquality
2024
Harvard University
2018-2023
Dana-Farber Cancer Institute
2017-2023
Massachusetts General Hospital
2020-2021
Moscow Research and Clinical Center for Neuropsychiatry
2018
Columbia University
2013
Abstract Despite improvements in survival among pediatric patients with acute lymphoblastic leukemia (ALL), outcomes for adolescents and young adults (AYAs) ALL have lagged. The reasons the inferior AYAs are multifactorial, each presenting unique challenges requiring novel solutions. First, adverse disease biology is more common ALL. Ongoing trials investigating approaches to treatment, such as incorporating JAK inhibitors Philadelphia chromosome–like ALL, menin KMT2A-rearranged BCL2/BCLXL...
2020 Background: The impact of individualized neoepitope vaccination targeting neoantigens arising from tumor-specific mutations for GBM, a low mutation burden tumor with an immunologically cold microenvironment, as well that concurrently administered dexamethasone (dex), are unknown. Methods: Individualized up to 20 synthetic, long peptides high predicted HLA binding affinity admixed poly-ICLC, were subcutaneously using prime-boost schedule after RT newly diagnosed, MGMT unmethylated, at...
Abstract Personal vaccines directed at neoantigens arising from tumor mutations can induce neoepitope-specific T cell responses in patients with highly mutated tumors such as melanoma. It remains unknown if this approach be successfully applied low mutation frequency. We conducted a phase 1/1b study to determine the safety and feasibility of patient-specific neoantigen-targeting vaccination newly diagnosed, methylguanine methyltransferase (MGMT) unmethylated glioblastoma, administered...
Autoimmune hemolytic anemia (AIHA) results in red blood cell destruction by auto-antibodies directed against surface antigens and is rarely fatal. Here we describe a case of AIHA, refractory to both standard experimental therapies, complicated multiorgan failure, rapidly leading death.A 65 year-old man who presented with progressive dyspnea jaundice was found have anemia. Diagnostic work-up revealed positive direct antiglobulin test strong pan-reactive antibody the plasma reacting titer...
Ruxolitinib, a selective inhibitor of Janus kinases 1 and 2, is increasingly being used in allogeneic hematopoietic cell transplantation (HCT) recipients following its approval by the U.S. Food Drug Administration for treatment steroid-refractory acute graft-versus-host disease. Although there extensive experience using ruxolitinib patients with myeloproliferative neoplasms, biologic effects clinical implications dosing, tapering, discontinuation HCT are incompletely characterized. We...
Abstract Obtaining paired alpha- and beta-chain information of the T cell receptor (TCR) enables identification which TCR binds to a particular antigen, providing critical knowledge for understanding how tumor interacts with its immune microenvironment monitoring responses immunotherapy. We have developed rapid, cost-effective single-cell sequencing protocol (scTCRseq) that processes single cells flow sorted into 384-well plates. Exploiting added specificity provided by RNase H-dependent PCR...
The impact of individualized neoepitope vaccination targeting neoantigens arising from tumor-specific mutations for GBM, a low mutation burden tumor with an immunologically cold microenvironment, as well that concurrently administered dexamethasone (dex), are unknown. Individualized up to 20 synthetic, long peptides high predicted HLA binding affinity admixed poly-ICLC, were subcutaneously using prime-boost schedule after RT newly diagnosed, MGMT unmethylated, at least partially resected,...
Abstract Recent advances in single cell T receptor (TCR) sequencing have allowed detailed analysis of TCRαβ pairs. However, efficient methods for probing the specificity discovered TCRs remain limited. We developed a streamlined approach cloning and expressing screening against candidate antigens. A key innovation was establishment plasmid library to encode all variable (V) regions TCR, from which any TCR interest can be assembled with only custom synthesis short CDR3 regions. reporter...