A5101497053- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- CAR-T cell therapy research
- MicroRNA in disease regulation
- Influenza Virus Research Studies
- Bladder and Urothelial Cancer Treatments
- Cancer-related molecular mechanisms research
- Lymphoma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Prostate Cancer Treatment and Research
- Urinary and Genital Oncology Studies
- Autophagy in Disease and Therapy
- Blood Pressure and Hypertension Studies
- Renal and Vascular Pathologies
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Phagocytosis and Immune Regulation
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Silicon Carbide Semiconductor Technologies
- PI3K/AKT/mTOR signaling in cancer
- Diabetes Treatment and Management
- Cutaneous lymphoproliferative disorders research
- Advanced Drug Delivery Systems
Shanghai Jiao Tong University
2021-2025
Shanghai First People's Hospital
2021-2025
Linyi People's Hospital
2019-2021
Guangxi University
2018-2019
Weifang Medical University
2018
Chinese Academy of Sciences
2008-2017
East China Normal University
2017
Center for Excellence in Molecular Cell Science
2017
Soochow University
2015-2017
Second Affiliated Hospital of Soochow University
2015-2017
T cells expressing a chimeric antigen receptor (CAR) engineered to target CD19 can treat leukemia effectively but also increase the risk of complications such as cytokine release syndrome (CRS) and CAR cell related encephalopathy (CRES) driven by interleukin-6 (IL-6). Here, we investigated whether IL-6 knockdown in CART-19 reduce secretion from monocytes, which may adverse events.Supernatants cocultures regular B lymphoma were added monocytes vitro, levels monocyte supernatants measured 24 h...
Abstract Background As the objective overall response rate to immune checkpoint inhibitors (ICIs) is less than 30% in late stage or metastatic bladder cancer (BLCA), elucidating intrinsic mechanisms of evasion great importance for discovery predictive and prognostic biomarkers exploration novel targets intervention. Recent studies have shown that OBSCN cytoskeletal protein it encodes, obscurin, play an important role tumour progression. However, no reported BLCA. Methods RNA sequencing...
Abstract Diabetic retinopathy (DR) presents a microvascular complication of diabetes, which may contribute to visual impairment. The treatment DR is still controversial. Accumulating studies have reported the role microRNAs (miRs) in DR. This study aims explore functions microRNA‐384‐3p (miR‐384‐3p) retinal neovascularization by targeting hexokinase 2 (HK2) mice with A total 43 C57BL/6 male were selected and divided into normal ( n = 16) 27) groups. Retinal endothelial cells (RMECs)...
Treatment with chimeric antigen receptor (CAR)-engineered T cells directed against the B-cell maturation (BCMA) promoted transient recovery from multiple myeloma (MM). However, absence of this on immature plasma may limit efficacy modality and facilitate relapse. The purpose study is to characterize a novel CAR that includes both single-chain variable fragment (scFv)-BCMA an scFv-CD19 in tandem orientation (tan-CAR) attempt target BCMA CD19 expression MM cells.The scFv sequences anti-CD19...
Abstract Background The production of transgenic chicken cells holds great promise for several diverse areas, including developmental biology and biomedical research. To this end, site-specific gene integration has been an attractive strategy generating cell lines successfully adopted inserting desired genes regulating specific expression patterns. However, optimization method is essential improving the efficiency genome modification in species. Results Here we compare knock-in methods based...
Abstract Chronic kidney disease (CKD) is a persistent structural and functional disorder. Quercetin one of active extracted flavonoids has protective effects. Therefore, we proposed to survey the effect on CKD. HK‐2 cells were preprocessed by then irritated with lipopolysaccharide (LPS). CCK‐8 assay flow cytometry utilized test viability apoptosis. ELISA was estimate IL‐6 TNF‐α secretion. Western blot performed examine expression apoptosis inflammation‐associated mediators. After that...
Urothelial carcinoma (UC) comprises a heterogeneous group of epithelial neoplasms with diverse biological behaviors and variable clinical outcomes. Distinguishing UC histological subtypes has become increasingly important because prognoses therapy can dramatically differ among subtypes. In work, overlapping morphological findings between low-grade noninvasive (LGNUC), which exhibits an inverted growth pattern, urothelial papilloma (IUP) make subclassification difficult. We propose...
Chronic kidney disease is a worldwide clinical and public health problem. As risk factor for cardiovascular cerebrovascular diseases, the has gradually become one of causes morbidity death. routine method screening disease, ultrasound very important in improving rate early detection accuracy chronic disease. This article explores value applying 3D conjunction with augmented reality medical technology observing changes tumor at different stages percutaneous patients Volunteers were selected...
The aim of the study was to explore correlation between rs7903146 and rs290487 polymorphisms in transcription factor 7-like 2 (TCF7L2) gene diabetic nephropathy (DN) Chinese Han population.Polymerase chain reaction-restriction fragment length polymorphism used determine genotypes TCF7L2 90 patients with DN 96 diabetes without DN. linkage disequilibrium (LD) haplotype analysis were performed haploview software. Hardy-Weinberg equilibrium assessed control group based on genotype distributions...
ssCART-19 cells with shRNA-IL-6 gene knockdown were subjected to a comprehensive safety evaluation, including efficacy, toxicity and biodistribution studies in NSG (PrkdcscidIL2rgtm1 /Bcgen) mice.NSG mice administered Raji-Luc then singly dosed via intravenous infusion. DNA fragments quantified different tissues by qPCR, the optical intensity of was determined for evaluate efficacy regular CAR-T cells. In study, clinical symptoms observation, body weight measurements, serum biochemical...
The Gleason grading system is a major tool used for prediction of prostate cancer (PCa) behavior. Because heterogeneity and sampling errors, prognosis variable even among patients with the same score (GS). Therefore, more accurate biomarkers that complement are needed to improve clinical management PCa.Formalin-fixed, paraffin embedded tissue samples were obtained from radical prostatectomy (RP) (patient set 1, n=53) needle biopsy 2, n=107; patient 3, n=119). Cancer tissues pure regions each...
Anaplastic lymphoma kinase-positive large B-cell (ALK <sup>+</sup> LBCL) is a rare variant of LBCL with an abnormal immunophenotype, which easily misdiagnosed as carcinoma or plasma cell neoplasm. Here, we present two cases ALK in the lymph node; describe their clinical, pathological, and immunophenotype features; compare them those reported cases.
Lupus nephritis (LN) is a kidney disorder that critical cause of mortality in patients with systemic lupus erythematosus. The present study aimed to explore the protective role complement component 1q (C1q) on LN and underlying mechanism involving nuclear factor (NF)‑κB singling pathway. MRL/lpr mice served as mouse model, pcDNA‑C1q was injected into determine C1q. C1q mRNA expression detected using reverse transcription‑quantitative PCR. Urine protein blood urea nitrogen (BUN) levels were...
Since the recent emergence of swine and avian flu, there has been a sense urgency to discover new anti-influenza drugs. In this study, stable cell line with M2 ion channel/ enhanced Green fluorescent protein (EGFP) co-expression was established in order develop an EGFP-based high-throughput assay for screening channel inhibitors. This directly monitors proton conductivity by measuring fluorescence intensity EGFP, which is dependent on sensitive pH. The ability amantadine inhibit detected...