A5079994961- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Genetic and phenotypic traits in livestock
- Virus-based gene therapy research
- Animal Nutrition and Physiology
- Reproductive Physiology in Livestock
- Renal and related cancers
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Birth, Development, and Health
- Xenotransplantation and immune response
- Heat shock proteins research
- Ovarian function and disorders
- Natural product bioactivities and synthesis
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- Reproductive System and Pregnancy
- Antibiotics Pharmacokinetics and Efficacy
- Nuclear Structure and Function
- Bee Products Chemical Analysis
- Reproductive biology and impacts on aquatic species
- Pharmacological Effects and Assays
Guangxi University
2015-2024
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2014-2024
Guangxi Center for Disease Prevention and Control
2023
AgroBio
2015-2022
Hubei University of Medicine
2020
University of Georgia
2011-2016
Institute of Subtropical Agriculture
2011
The recent development of porcine induced pluripotent stem cells (piPSCs) capable generating chimeric animals, a feat not previously accomplished with embryonic or iPSCs in species outside rodents, has opened the doors for in-depth study iPSC tumorigenicity, autologous transplantation, and other key aspects to safely move therapies clinic. tumorigenicity is critical as previous research mouse showed that iPSC-derived chimeras possessed large numbers tumors, rising significant concerns about...
The selection for improved body weight is an effective approach in animal breeding. Guangxi Partridge chickens have differentiated into two lines under selective breeding, which include line S and D that shown statistically significant differences weight. However, the meat quality analysis our study indicated of breast thigh muscles changed, included increased values L*, b*, drip loss decreased a* value, pH, shear force skeletal muscles. To illuminate effect on muscles, LC-MS/MS metabolomics...
Spermatogenesis carries the task of precise intergenerational transmission genetic information from paternal genome and involves complex developmental processes regulated by testicular microenvironment. Studies performed mainly in mouse models have established theoretical basis for spermatogenesis, yet wide interspecies differences preclude direct translation findings, farm animal studies are progressing slowly. More than 32,000 cells prepubertal (3-month-old) pubertal (24-month-old) buffalo...
Avian species are important model animals for developmental biology and disease research. However, unlike in mice, where clonal lines of pluripotent stem cells have enabled researchers to study mammalian gene function, highly proliferative avian cell been an elusive goal. Here we demonstrate the generation induced (iPSCs), first nonmammalian iPSCs, which were clonally isolated propagated, attributes not attained embryo-sourced cells. This was accomplished using human pluripotency genes...
l-Carnitine (LC) is considered to be a natural antioxidant agent that could used improve the efficiency of reproduction. However, precise machinery effect LC supplementation on frozen-thawed rabbit sperm has not been evaluated. Thus, aim this study was evaluate addition freezing medium parameters and ultrastructure changes in sperm. Rabbit bucks (7 months age) were involved, semen collected using artificial vagina method. Pooled cryopreserved tris yolk fructose extender without any...
Exosomes are nanosized membranous vesicles secreted by various types of cells, which facilitate intercellular communication transporting bioactive compounds. abundant in biological fluids including semen, and their protein composition the potential seminal plasma exosomes (SPEs) as fertility biomarkers were elucidated humans, however, little information is available regarding buffalo (Bubalus bubalis). Here, we examined correlation between spermatozoa, (SP), SPEs, compared analyzed...
Follicular fluid (FF) accumulates in the antrum of ovarian follicle and provides microenvironment for oocyte development. FF plays an important role growth maturation. The a unique window to investigate processes occurring during buffalo follicular observed low quality oocytes may arise from poor microenvironment. Investigating proteins found (BFF) should provide insight into development further understanding intra-follicular maturation quality. Here, proteomic-based approach was used...
This study aimed to evaluate the effects of dietary Lasia spinosa Thw. (LST) powder supplementation on growth performance, blood metabolites, antioxidant status, intestinal morphology, and cecal microbiome in broiler chickens. A total 400 1-day-old male Guangxi partridge broilers (initial body weight: 42.52 ± 0.06 g) were randomly allotted 4 treatments: LST0 group (a basal diet), LST1 diet with 1% LST powder), LST2 2% LST4 4% 10 replicates for each treatment, treatment group. Results...
Germ cells (GCs) are critically important as the vehicle that passes genetic information from one generation to next. Correct development of these is essential and perturbation in their often leads reproductive failure disease. Despite importance GCs, little known about mechanisms underlying acquisition maintenance GC character. Using a reprogramming strategy, we demonstrate overexpression ectopic transcription factors embryonic fibroblasts can lead chicken induced primordial germ (ciPGCs)....
Siraitia grosvenorii is a kind of medicinal food plant. The mogroside-rich extract (MGE) its fruits can effectively ameliorate obesity, but the underlying mechanisms remain underexplored. In this study, we aimed to determine whether MGE obesity by protecting against divergences gut microbiota. Mice were challenged with high-fat diet (HFD) and treated oral gavage. Then, characteristics microbiota determined 16S rDNA analysis. Our findings showed that could significantly reduce body weight...
Xenotransplantation is a rapidly expanding field of research, and cloned miniature pigs are considered to be good model animals for its development. Although many animal species have been cloned, the success rate very low, especially in pig. To optimize protocols somatic cell nuclear transfer Guangxi Bama minipig, relationship between cycle synchronization histone acetylation levels were investigated. The results showed that cells efficiently synchronized by either serum starvation or...
Autologous bone grafting is the most effective treatment for long-bone nonunions, but it poses considerable risks to donors, necessitating development of alternative therapeutics. Poly(ethylene glycol) (PEG) microencapsulation and BMP2 transgene delivery are being developed together induce rapid formation. However, methods make these treatments available clinical applications presently lacking. In this study we used mesenchymal stem cells (MSCs) due their ease harvest, replication potential,...
Abstract Background The production of transgenic chicken cells holds great promise for several diverse areas, including developmental biology and biomedical research. To this end, site-specific gene integration has been an attractive strategy generating cell lines successfully adopted inserting desired genes regulating specific expression patterns. However, optimization method is essential improving the efficiency genome modification in species. Results Here we compare knock-in methods based...
In this study, the effects of 3 graded dietary levels (0.1%, 0.2%, and 0.4%) naringin were studied in Three-Yellow breeder hens during late laying period (55-62 wk). A total 480 (54-wk-old) randomly divided into 4 groups (6 replicates 20 hens): basal diet group (C), diets supplemented with 0.1%, 0.4% (N1, N2, N3), respectively. Results showed that supplementation for 8 wk increased rate egg mass, enhanced yolk color, decreased feed ratio (P < 0.05). Meanwhile, compared C group, there more...
Abstract Maternal secretion of recombinant proteins into chicken eggs may provide a viable approach for pharmaceutical production but remains limited by poor efficiency through the membrane oviduct cells, despite high expression levels. Here, we used site-specific integration an EGFP fused to OVAL gene rigid linker, (EAAAK) 3 , at endogenous ovalbumin locus in primordial germ cells generate OVAL-E3-EGFP transgenic chickens, with chickens expressing CMV immediate enhancer/β-actin-driven...