A5101728432- Mosquito-borne diseases and control
- Malaria Research and Control
- Invertebrate Immune Response Mechanisms
- Complement system in diseases
- Immune Cell Function and Interaction
- Insect symbiosis and bacterial influences
- HIV Research and Treatment
- Gut microbiota and health
- Insect Resistance and Genetics
- Immunotherapy and Immune Responses
- RNA regulation and disease
- Autophagy in Disease and Therapy
- Genetic factors in colorectal cancer
- Neurological diseases and metabolism
- Probiotics and Fermented Foods
- CAR-T cell therapy research
- Advanced Biosensing Techniques and Applications
- Toxoplasma gondii Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Hereditary Neurological Disorders
- Selenium in Biological Systems
- Advancements in Transdermal Drug Delivery
- Pulmonary Hypertension Research and Treatments
- Prostate Cancer Treatment and Research
- Mercury impact and mitigation studies
Army Medical University
2015-2025
Hangzhou City University
2025
Wenzhou Medical University
2024
Tongji University
2021
Xiamen University
2009-2015
Chongqing University of Technology
2014
Plastic Surgery Hospital
2011
Peking Union Medical College Hospital
2011
Fred Hutch Cancer Center
2005
Plasmodium yoelii is an excellent model for studying malaria pathogenesis that often intractable to investigate using human parasites; however, genetic studies of the parasite have been hindered by lack genome-wide linkage resources. Here, we performed 14 crosses between three pairs P. clones/subspecies, isolated 75 independent recombinant progeny from crosses, and constructed a high-resolution map this parasite. Microsatellite genotypes formed groups belonging chromosomes, allowing...
Abstract Background Thioester-containing proteins (TEPs) serve as crucial effectors and regulatory components within the innate immune system of mosquitoes. Despite their significance, mechanisms by which TEPs exert negative regulation on response in mosquitoes remain inadequately understood. This study aims to elucidate role melanization, thereby advancing our comprehension function response. Methods We infected female Anopheles stephensi with Plasmodium yoelii allowing them feed...
Background Early diagnosis and treatment for encephalitis are crucial improving patient outcomes reducing the economic burden, especially given overlapping symptoms low specificity of auxiliary diagnostic tests between viral (VE) autoimmune (AE). Since these two conditions require different approaches, an early differential AE VE is a critical challenge. Methods This study enrolled cohort 75 patients (38 with 37 AE) September 2022 July 2024. The demographic data, clinical characteristics,...
Abstract TLRs (Toll-like receptors) play an important role in the initiation of innate immune responses against invading microorganisms. Although several have been reported to be involved response blood-stage malaria parasites, development pre-erythrocytic stage is still largely unknown. Here, we found that sporozoite and its lysate could significantly activate TLR2 induce macrophages release proinflammatory cytokines, including IL-6, MCP-1 TNF-α, a TLR2-dependent manner. Further studies...
Malaria parasites are less vulnerable to mosquito immune responses once ookinetes transform into oocysts, facilitating parasite development in the mosquito. However, underlying mechanisms of oocyst resistance defenses remain unclear. Here, we show that circumsporozoite protein (CSP) is required for rodent malaria oocysts avoid defenses. Mosquito infection with CSPmut (mutation CSP pexel I/II domains) induces nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 5 (NOX5)-mediated...
Malaria parasites suppress host immune responses to facilitate their survival, but the underlying mechanism remains elusive. Here, we found that blood-stage malaria predominantly induced CD4+Foxp3+CD25+ regulatory T cells release soluble fibrinogen-like protein 2 (sFGL2), which substantially enhanced infection. This was attributed capacity of sFGL2 inhibit macrophages from releasing monocyte chemoattractant protein-1 (MCP-1) and sequentially reduce recruitment natural killer/natural killer...
Cancer vaccines that rely on tumor antigen-specific CD8+ T cell responses, are promising anti-cancer adjuvant immunotherapies. This study investigated whether genetically attenuated Plasmodium sporozoite (GAS) could be used as a novel vector to induce responses against lung cancer.We constructed GAS/MAGE-A3, recombinant GAS engineered express the cancer-specific antigen, melanoma-associated antigen 3 (MAGE-A3), and assessed its therapeutic effects cancer.Robust parasite-specific...
ABSTRACT One unique feature of malaria parasites is the differential transcription structurally distinct rRNA (rRNA) genes at different developmental stages: A-type are transcribed mainly in asexual stages, whereas S-type expressed mostly sexual or mosquito stages. Conclusive functional evidence rRNAs regulating stage-specific parasite development, however, still absent. Here we performed genetic crosses Plasmodium yoelii with one parent having an oocyst development defect (ODD) phenotype...
Intratumor heterogeneity is a key driver for local relapse and treatment failure. Thus, using multifocal prostate cancer as model to investigate tumor inter-clonal relationships evolution could aid in our understanding of drug resistance. Previous studies discovered genomic alterations by comparing hormone-sensitive (HSPC) with castration-resistant (CRPC) large cohorts. However, most did not sequentially sample tumors from the same patient. In study, we performed whole-exome sequencing (WES)...
T follicular helper (TFH) cells are fundamental for the development of humoral immunity. In our previous study, we found that PD-1 deficiency substantially promoted expansion Plasmodium-specific TFH and enhanced immunity ITV (infection treatment vaccine)-immunized mice. However, underlying mechanism by which signaling modulates activation remains unclear.Mice were immunized with following standard procedures. The phenotype CD11c+CXCR5+ dendritic (DCs), frequency number splenic regulatory...
Although exo-erythrocytic forms (EEFs) of liver stage malaria parasite in the parasitophorous vacuole (PV) are encountered with robust host innate immunity, EEFs can still survive and successfully complete infection hepatocytes, underlying mechanism is largely unknown. Here, we showed that sporozoite circumsporozoite protein (CSP) translocated from into hepatocyte cytoplasm significantly mediated resistance to killing by interferon-gamma (IFN-γ). Attenuation IFN-γ-mediated CSP was dependent...
As a new type of biomaterials, gold nanoparticles (GNPs), also known as colloidal (CG), have wide biomedical application. In this study, GNPs with diameters 10, 15, and 25 nm were prepared by sodium citrate reduction, detected common
Abstract As a malaria transmission-blocking vaccine alone does not confer direct benefit to the recipient, it is necessary develop that only blocks transmission but also protects vaccinated individuals. In this study we observed whole-killed blood-stage (WKV) conferred protection against challenge markedly inhibited of different strains parasite. Although parasitemia much lower in WKV-immunized mice challenged with parasites, gametocytemia comparable between control and immunized during...
Although the attenuated sporozoite is most efficient vaccine to prevent infection with malaria parasite, limitation of a source sterile sporozoites greatly hampers its application. In this study, we found that whole-killed, blood-stage lysate could confer protection against blood stage as well liver stage. protective immunity induced by whole-organism dependent on parasite-specific CD4+ T-cell responses and antibodies, in mice immunized vaccine, CD8+ , but not effector contributed Thus, our...
The transmission of drug-resistant parasites by the mosquito may be influenced altered biological fitness and different immune reactions or metabolic change in mosquito. At this point, little is known about variations immunity metabolism when mosquitoes are infected with parasites. To understand differential gene expression Anopheles following infection Plasmodium, we conducted a genome-wide transcriptomic profiling analysis stephensi feeding on mice drug-sensitive P. yoelii, observed...
Charcot-Marie-Tooth disease (CMT) is a hereditary peripheral neuropathy involving approximately 80 pathogenic genes. Whole-exome sequencing (WES) and confirmatory Sanger analysis was applied to identify the disease-causing mutations in Chinese patient with lower limb weakness. We present an 18-year-old male 2.5-year history of progressive weakness unsteady gait. Upon admission, physical examination revealed hands tremulousness, bilateral calf muscle wasting weakness, pes cavus, elevated...
Abstract A whole‐killed malaria blood‐stage vaccine (WKV) is promising in reducing the morbidity and mortality of patients, but its efficacy needs to be improved. We found that antimalarial drug chloroquine could augment protective WKV Plasmodium yoelii . The direct effect on parasites during immunization excluded, as administration or plus alum every two weeks had a slight parasitemia, an with NP‐KLH (4‐hydroxy‐3‐nitrophenylacetyl Keyhole Limpet Hemocyanin) significantly promote generation...
Objective To investigate the effect of Astragalus polysaccharides (APS) on 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats and dendritic cells (DCs) mesenteric lymph nodes.Methods Forty-four male Sprague-Dawley were randomly divided into four groups (n = 11) using simple random sampling: normal control group, TNBS APS 5-aminosalicylic (5-ASA) group.Experimental was induced by enema last three groups.Rats 5-ASA treated gavage with (0.75 g ? kg-1 d-1) (100 mg 10 consecutive...
Abstract Although exoerythrocytic forms (EEFs) of liver stage malaria parasite in parasitophorous vacuole (PV) encountered with robust host innate immunity, EEFs can still survive and successfully complete infection hepatocytes, the underlying mechanism is largely unknown. Here, we showed that sporozoite circumsporozoite protein (CSP) translocated from into hepatocyte cytoplasm significantly inhibited killing exo-erythrocytic by interferon-gamma (IFN-γ). Attenuation IFN-γ-mediated CSP was...