A5005661061- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Dermatology and Skin Diseases
- Asthma and respiratory diseases
- Contact Dermatitis and Allergies
- Plant Reproductive Biology
- Urticaria and Related Conditions
- Celiac Disease Research and Management
- Insect and Pesticide Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Wheat and Barley Genetics and Pathology
- Monoclonal and Polyclonal Antibodies Research
- Drug-Induced Adverse Reactions
- Plant Molecular Biology Research
- Microscopic Colitis
- Biochemical and Structural Characterization
- Cellular Mechanics and Interactions
- Transgenic Plants and Applications
- Signaling Pathways in Disease
- Mast cells and histamine
- Studies on Chitinases and Chitosanases
- Bacteriophages and microbial interactions
- Parasitic Diseases Research and Treatment
- thermodynamics and calorimetric analyses
Medical University of Vienna
2010-2020
Christian Doppler Laboratory for Thermoelectricity
2010-2016
Vienna General Hospital
1995-2013
Ludwig-Maximilians-Universität München
2006
University of Vienna
1995-2004
Philipps University of Marburg
2004
Vienna Biocenter
1996
Background Atopic dermatitis (AD) is a complex chronic inflammatory disease where allergens can act as specific triggering factors. Aim To characterize the specificities of IgE-reactivity in patients with AD to broad panel exogenous including microbial and human antigens. Methodology Adult were grouped according SCORAD index, into severe (n = 53) moderate 126). As controls 43 included seborrhoeic eczema 97 individuals without history allergy or skin diseases. Specific IgE reactivity was...
Abstract Background IgE reactivity to antigens from Gram‐positive and Gram‐negative bacteria is common in patients suffering respiratory skin manifestations of allergy, but the routes mechanisms sensitization are not fully understood. The analysis genome, transcriptome microbiome house dust mites ( HDM ) has shown that Staphylococcus aureus S. Escherichia coli E. species abundant within microbiome. Therefore, our aim was investigate whether carriers bacterial leading atopic dermatitis....
Abstract Skin inflammation in atopic dermatitis starts with Th2 and IgE-mediated responses against exogenous allergens and, for unknown reasons, resembles features of a Th1-driven reaction the chronic stages. We report characterization human protein, Hom s 4, recognized by IgE autoantibodies from patients. The complete 4 cDNA codes 54-kDa basic protein containing two typical calcium-binding domains separated an unusually long α-helical domain. Therefore, homologous proteins found sequence...
Abstract Autoreactivity may play a critical role in the chronification of atopic dermatitis (AD). Several studies showed that AD patients produce IgE Abs specific for autoantigens, and we described Th as well CD8+ T cells autoallergen Hom s 2, α-chain nascent polypeptide-associated complex (α-NAC). This study aimed to investigate frequency inflammatory phenotype autoallergen-specific cells. cell immunodominant epitopes α-NAC were mapped by applying prediction softwares, binding affinity was...
In high-risk populations, allergen-specific prophylaxis could protect from sensitization and subsequent development of allergic disease. However, such treatment might itself induce allergies, thus requiring hypoallergenic vaccine formulations. We here characterized the preventive potential virus-like nanoparticles (VNP) expressing surface-exposed or shielded allergens.Full-length major mugwort pollen allergen Art v 1 was selectively targeted either to surface inner side lipid bilayer...
Background A subgroup of patients with atopic dermatitis (AD) produces IgE autoantibodies to human proteins which may be present in inflamed skin and perpetuate cutaneous inflammation. Objectives In order investigate mechanisms 'autoallergy' for AD we studied T‐cell responses the autoallergen Hom s 2, transcriptional coactivator α‐nascent polypeptide‐associated complex (α‐NAC). Methods Specific proliferation blood lymphocytes from 30 12 healthy control individuals was investigated by flow...
Wheat (Triticum aestivum) is an important allergen source responsible for various clinical manifestations of allergy (i.e. food allergy, pollen respiratory to flour-Baker's asthma).The objective this study was the molecular and immunological characterization new recombinant wheat allergens evaluate their usefulness diagnosis wheat.A T. aestivum cDNA library constructed screened with serum IgE from patients suffering identify cDNAs coding allergens. The allergen-encoding were expressed in...
Background The house dust mite (HDM) allergen Der p 18 belongs to the glycoside hydrolase family chitinases. relevance of for allergic patients has only been partly investigated. Objective To perform a detailed characterization on molecular, structural and immunological level. Methods was expressed in E. coli, purified homogeneity, tested chitin-binding activity its secondary structure analyzed by circular dichroism. 18-specific IgG antibodies were produced rabbits localize mites using...
Background Allergy to bee and wasp venom can lead life-threatening systemic reactions. The identification of the culprit species is important for allergen-specific immunotherapy. Objectives To determine a panel recombinant allergens which suitable or as allergen sources search molecular surrogates clinical severity sting Methods Sera from eighty-seven patients with detailed documentation their reaction (Mueller grade) who had been subjected titrated skin testing were analyzed wasp-specific...