A5030886659- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Allergic Rhinitis and Sensitization
- Renal cell carcinoma treatment
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- CAR-T cell therapy research
- Cancer Research and Treatments
- Food Allergy and Anaphylaxis Research
- Immune cells in cancer
- Vector-Borne Animal Diseases
- vaccines and immunoinformatics approaches
- Herpesvirus Infections and Treatments
- Retinoids in leukemia and cellular processes
- Pancreatic and Hepatic Oncology Research
- RNA Interference and Gene Delivery
- Estrogen and related hormone effects
- Virus-based gene therapy research
- Adenosine and Purinergic Signaling
- Glioma Diagnosis and Treatment
- Research on Leishmaniasis Studies
Boehringer Ingelheim (Austria)
2018-2023
University of Regensburg
2009-2017
Center for Cancer Research
2006-2009
National Cancer Institute
2006-2009
National Institutes of Health
2006-2009
GlobeImmune (United States)
2007
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2002-2003
University of Salzburg
1999-2003
Comenius University Bratislava
2003
Slovak Academy of Sciences
2002
Drugs targeting the programmed cell death protein 1 (PD-1) pathway are approved as therapies for an increasing number of cancer entities, including renal carcinoma. Despite a significant increase in overall survival, most treated patients do not show durable clinical responses. A combination checkpoint inhibitors could provide promising improvement. The aim study was to determine blockade carcinoma patients. Tumor-infiltrating lymphocytes (TIL) and autologous peripheral blood mononuclear...
TNF and receptor type 2 (TNFR2) have been shown to be important for generation of myeloid-derived suppressor cells (MDSC). In order analyze whether how TNFR2 passes the effect on, myeloid from TNFR2-deficient mice were compared respective wild-type mice. Primary showed reduced production NO IL-6 which was attributable CD11b(+) CD11c(-) Ly6C(+) Ly6G(-) immature monocytic MDSC. MDSC isolated bone marrow less suppressive T cell proliferation WT-derived These differences on between two mouse...
Abstract The biological role of Langerin+ dendritic cells (DCs) such as Langerhans and a subset dermal DCs (dDCs) in adaptive immunity against cutaneous pathogens remains enigmatic. Thus, we analyzed the impact T cell-mediated toward Leishmania major parasites Lang-DTR mouse model that allows conditional diphtheria toxin (DT)-induced ablation vivo. For first time, infection experiments with DT-treated mice revealed proliferation L. major-specific CD8+ is significantly reduced during early...
Immunosuppression, impaired cytokine production and high susceptibility to secondary infections are characteristic for septic patients, mice after induction of polymicrobial peritonitis by sublethal cecal ligation puncture (CLP). Here, we demonstrate that CLP markedly altered subsequent B-cell responses. Total IgG IgM levels, as well the memory response, were increased in mice, but antigen-specific primary antibody was strongly impaired. We found two days CLP, CD11b(+) splenocytes activated...
Upregulation of inhibitory receptors, such as lymphocyte activation gene-3 (LAG-3), may limit the antitumor activity therapeutic antibodies targeting programmed cell death protein-1 (PD-1) pathway. We describe binding properties ezabenlimab, an anti-human PD-1 antibody, and BI 754111, LAG-3 assess their alone in combination. Ezabenlimab bound with high affinity to human (KD = 6 nM) blocked interaction PD-L1 PD-L2. dose-dependently increased interferon-γ secretion T cells expressing...
Abstract Despite some impressive clinical results with immune checkpoint inhibitors, the majority of patients cancer do not respond to these agents, in part due immunosuppressive mechanisms tumor microenvironment. High levels adenosine tumors can suppress cell function, and strategies target pathway involved its production have emerged. CD73 is a key enzyme production. This led us identify novel humanized antagonistic antibody, mAb19, distinct binding properties. mAb19 potently inhibits...
ABSTRACT The circumsporozoite protein (CSP) from the surface of sporozoite stage Plasmodium sp. malaria parasites is among most important vaccine candidates. Gene gun injection genetic vaccines encoding berghei CSP induces a significant protective effect against challenge; however, intramuscular does not. In present study we compared immune responses and effects induced by P. delivered intradermally with needle or epidermally gene gun. Mice were immunized three times at 4-week intervals...
IL-2 is well described as a cytokine with two markedly distinct functionalities: necessary signal during CD4(+) and CD8(+) T cell activation/expansion an essential for the maintenance of CD4(+)CD25(+)FoxP3(+) cells (regulatory (T(REG)) cells) homeostasis. In this study we demonstrate first time that, compared use alone, complex anti-IL-2 Ab (IL-2 complex) enhances effectiveness viral vaccine in mouse model known Ag specificity. led to increase number Ag-specific effector/memory cells,...
<b>Background:</b> Recent publications indicate that immunization with plasmid DNA encoding allergens might represent a potential approach in allergen–specific immunotherapy. <b>Objective:</b> In the present study we have compared immune responses induced by for two isoforms of Bet v 1, major allergen birch pollen. <b>Methods:</b> BALB/c mice were injected intradermally genes 1a (pCMV–Beta) and 1d (pCMV–Betd). addition, effect immunostimulatory sequences...
The C terminus of the circumsporozoite protein (CSP) is anchored to parasite cell membrane by a glycosylphosphatidylinositol (GPI) glycolipid. This GPI signal sequence functions poorly in heterologous eukaryotic cells, causing CSP retention within internal organelles during genetic immunization. Cellular location antigen has quantitative and qualitative effects on immune responses induced Removal had profound effect induction efficacy CSP-specific response after immunization BALB/c mice with...
TNF receptor type 2 (TNFR2) has gained attention as a costimulatory for T cells and critical factor the development of regulatory (Treg) myeloid suppressor cells. Using TNFR2-specific agonist TNCscTNF80, direct effects TNFR2 activation on were investigated in mice. In vitro, TNCscTNF80 induced cell proliferation fashion, also supported vitro expansion Treg addition, retarded differentiation bone marrow-derived immature culture reduced their function. vivo application TNCscTNF80-induced mild...
VISTA (PD-1H) is an immune regulatory molecule considered part of the next wave immuno-oncology targets. immunoglobulin (Ig) superfamily cell surface mainly expressed on myeloid cells, and to some extent NK cells T cells. In previous preclinical studies, VISTA-targeting antibodies provided inhibitory signals, while other triggered stimulatory signals. Importantly, for therapeutic antibodies, isotype backbone can have a strong impact antibody function. To elucidate mode action anti-VISTA we...
Sepsis-induced immune reactions are reduced in TNF receptor 2 (TNFR2)-deficient mice as previously shown. In order to elucidate the underlying mechanisms, functional integrity of myeloid cells TNFR2-deficient was analyzed and compared wild type (WT) mice. The capacity dendritic produce IL-12 strongly impaired TNF-deficient mice, mirroring production by WT sepsis or after LPS pre-treatment. addition, were refractory pre-treatment also hyper-sensitization inactivated Propionibacterium acnes,...
The immune system in sepsis is impaired as seen by reduced numbers and function of cells antigen-specific antibody responses. We studied T cell septic mice using cecal ligation puncture (CLP) a clinically relevant mouse model for sepsis. proliferative response CD4(+) CD8(+) was suppressed mice. Adoptive transfer experiments demonstrated that the were not intrinsically altered CLP. Instead, host environment responsible this suppression. While CLP-induced suppression dependent on TNF activity,...