A5017170291- Immune Response and Inflammation
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Sepsis Diagnosis and Treatment
- Adrenal Hormones and Disorders
- Intramuscular injections and effects
- Neuroinflammation and Neurodegeneration Mechanisms
- Platelet Disorders and Treatments
- Heparin-Induced Thrombocytopenia and Thrombosis
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Amoebic Infections and Treatments
- Vitamin C and Antioxidants Research
- Immunotherapy and Immune Responses
- Metabolism and Genetic Disorders
- Urinary Bladder and Prostate Research
- Gastrointestinal motility and disorders
- Pancreatitis Pathology and Treatment
- Hormonal and reproductive studies
- Prostate Cancer Diagnosis and Treatment
- Diabetes Management and Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
Universitätsmedizin Greifswald
2007-2020
Johns Hopkins University
2017
Universität Greifswald
2010-2011
Immunosuppression, impaired cytokine production and high susceptibility to secondary infections are characteristic for septic patients, mice after induction of polymicrobial peritonitis by sublethal cecal ligation puncture (CLP). Here, we demonstrate that CLP markedly altered subsequent B-cell responses. Total IgG IgM levels, as well the memory response, were increased in mice, but antigen-specific primary antibody was strongly impaired. We found two days CLP, CD11b(+) splenocytes activated...
The role of regulatory T cells (Tregs) in bacterial sepsis remains controversial because antibody-mediated depletion experiments gave conflicting results. We employed DEREG mice (DEpletion REGulatory cells) and a caecal ligation puncture model to elucidate the CD4+Foxp3+ Tregs sepsis. In natural can be visualized easily selectively depleted by diphtheria toxin animals express receptor enhanced green fluorescent protein as fusion under control foxp3 locus. confirmed rapid Treg-activation an...
Background and Purpose . Regulatory T cells (Tregs) have been suggested to modulate stroke-induced immune responses. However, analyses of Tregs in patients experimental stroke yielded contradictory findings. We performed the current study assess regulation function peripheral blood patients. Age dependent expression CD39 on was quantified mice men. Methods Total FoxP3 + were by flow cytometry controls admission days 1, 3, 5, 7 thereafter. Treg assessed quantifying inhibition...
To investigate the role of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) in postoperative polymicrobial abdominal sepsis.Sepsis is leading cause death among critically ill surgical patients. TRAIL commonly known as an agent cancer cells. It also plays important regulation inflammatory reactions. The sepsis still unclear.Experimental animal model.University laboratory.C57BL/6 mice.Colon ascendens stent peritonitis (CASP) was induced female mice. One hour, 24 hrs, and...
Abdominal sepsis due to intestinal leakage of endogenous gut bacteria is a life-threatening condition. In healthy individuals, T lymphocytes have essential functions in balancing the immune response commensal flora.To determine how shape process diffuse faecal peritonitis.In colon ascendens stent peritonitis (CASP), clinically relevant mouse model peritonitis, kinetics systemic cell activation were investigated by assessment markers. CD4(+) cells then depleted with monoclonal antibodies, and...
During sepsis, CD4 T cells express activation markers within the first 24 h. In present study, mechanisms of T-cell and its consequences were addressed in an acute peritonitis model mice. The response CD4+ to sepsis induction was compared between OTII mice, characterized by ovalbumin-specific receptor-transgenic cells, C57BL/6 controls (wild type [WT] mice). Because ovalbumin absent during peritonitis, could not be activated canonical antigen recognition. both WT control effector Foxp3+...
Patients with sepsis are often immune suppressed, and experimental mouse models of also display this feature. However, acute in mice is characterized by a generalized B cell activation plasma differentiation, resulting marked increase serum antibody concentration. Its effects on humoral memory not clearly defined. We measured the long-term immunological for defined antigen: we induced colon ascendens stent peritonitis (CASP) 8 weeks after 2 rounds immunization ovalbumin. Four later,...
Sepsis is frequently complicated by a state of profound immunosuppression, in its extreme form known as immunoparalysis. We have studied the role adaptive immune system murine acute peritonitis model. To read out we primed post-septic and control animals immunization with model antigen TNP-ovalbumin alum, measured specific antibody-responses via ELISA ELISpot assay well T-cell responses proliferation after restimulation. Specific antibody titers, affinity plasma cell counts bone marrow were...
Although antigen-specific priming of antibody responses is impaired during sepsis, there nevertheless a strong increase in IgM and IgG serum concentrations. Using colon ascendens stent peritonitis (CASP), mouse model polymicrobial abdominal we observed substantial increases as well all subclasses, starting at day three peaking two weeks after sepsis induction. The dominant source antibody-secreting cells was by far the spleen, with minor contribution mesenteric lymph nodes. Remarkably,...
In murine abdominal sepsis by colon ascendens stent peritonitis (CASP), a strong increase in serum IgM and IgG antibodies was observed, which reached maximum values 14 days following induction. The specificity of this antibody response studied at the single cell level using broad panel bacterial, sepsis-unrelated as well self-antigens. Whereas an antibacterial IgM/IgG rarely studies single-cell revealed that antibodies, particular, were largely polyreactive. Interestingly, least 16% mAbs 20%...
Monocyte chemotactic protein-1 (MCP-1) is differentially expressed in benign prostatic hyperplasia (BPH) and a potential clinical biomarker for BPH.We collected urine post-digital rectal examination (DRE) from 48 men who did not have diagnosis of prostate cancer.We measured MCP-1 levels by enzyme-linked immunosorbent assay (ELISA) compared them with weight lower urinary tract symptoms (LUTS) as the International Prostate Symptom Score (I-PSS) utilizing correlation regression analyses.In...