A5027630612- Sepsis Diagnosis and Treatment
- Infection Control in Healthcare
- Immune Response and Inflammation
- Cancer, Stress, Anesthesia, and Immune Response
- Surgical site infection prevention
- Surgical Sutures and Adhesives
- Pancreatitis Pathology and Treatment
- Appendicitis Diagnosis and Management
- Thermal Regulation in Medicine
- Vitamin C and Antioxidants Research
- Intraperitoneal and Appendiceal Malignancies
- Venous Thromboembolism Diagnosis and Management
- Neuroblastoma Research and Treatments
- Patient Satisfaction in Healthcare
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Neurofibromatosis and Schwannoma Cases
- Vagus Nerve Stimulation Research
- Neuropeptides and Animal Physiology
- Immune cells in cancer
- Adrenal and Paraganglionic Tumors
- Intensive Care Unit Cognitive Disorders
- Ultrasound and Hyperthermia Applications
- Patient-Provider Communication in Healthcare
- Plasma Applications and Diagnostics
- Intestinal and Peritoneal Adhesions
Universitätsmedizin Greifswald
2012-2022
Universität Greifswald
2006-2013
Moritz Klinik
2010
The rate of microscopic incomplete resections gastrointestinal cancers including pancreatic cancer has not changed considerably over the past years. Future intra-operative applications tissue tolerable plasmas (TTP) could help to address this problem. Plasma is generated by feeding energy, like electrical discharges, gases. development non-thermal atmospheric displaying spectra temperature within or just above physiological ranges allows biological medical plasmas. We have investigated...
// Lars Ivo Partecke 1, * , André Käding Dung Nguyen Trung 1 Stephan Diedrich Matthias Sendler 2 Frank Weiss Jens-Peter Kühn 3 Julia Mayerle Katharina Beyer 4 Wolfram von Bernstorff Claus-Dieter Heidecke Keßler Department of General, Visceral, Thoracic and Vascular Surgery, University Medicine, Greifswald, Germany Internal Medicine A, Experimental Radiology, Current address: Visceral Charité–University Campus Benjamin Franklin, Berlin, These authors...
Negative pressure wound therapy (NPWT) is an established treatment option, but there no evidence of benefit for subcutaneous abdominal healing impairment (SAWHI).To evaluate the effectiveness and safety NPWT SAWHI after surgery in clinical practice.The multicenter, multinational, observer-blinded, randomized study enrolled patients between August 2, 2011, January 31, 2018. The last follow-up date was June 11, trial included 34 surgical departments hospitals Germany, Belgium, Netherlands, 539...
Pancreatic cancer is the fourth leading cause of tumour death in western world. However, appropriate models are scarce. Here we present a syngeneic murine pancreatic model using 7 Tesla MRI and evaluate its clinical relevance applicability.6606PDA cells were orthotopically injected into head. Liver metastases induced through splenic injection. Animals analyzed by three five weeks following Tumours detected T2-weighted high resolution sequences. Tumour volumes determined callipers MRI....
<b><i>Background:</i></b> In postoperative sepsis, mortality is increased due to the surgically induced immune dysfunction. Further causes of this traumatic effect on system include burn injuries and polytrauma, as well endogenous traumata like stroke. Several animal models have been defined analyse characteristics trauma-induced suppression. This article will correlate our results from studies clinical observations with recent literature...
Poor prognosis of pancreatic cancer has remained almost unchanged in recent years. Cold physical plasma was suggested as an innovative anticancer strategy, but its selective killing activity malignant over non-malignant cells only partially been explored. The present study aimed at exploring the effect cold on cellular viability.Induction cell death and apoptosis by investigated murine PDA6606 primary fibroblasts vitro (2D 3D cultures) ovo.Plasma increased to a significantly higher extent...
Sepsis remains a persistent problem on intensive care units all over the world. Understanding complex mechanisms of sepsis is precondition for establishing new therapeutic approaches in this field. Therefore, animal models are required that able to closely mimic human disease and also sufficiently deal with scientific questions. The Colon Ascendens Stent Peritonitis (CASP) highly standardized model polymicrobial abdominal rodents. In model, small stent surgically inserted into ascending...
Objective During abdominal sepsis, the activation of hepatic Kupffer cells (KC) and its consequences are central interest. This study evaluates impact selective KC depletion on microcirculation, cytokine release, systemic alterations in colon ascendens stent peritonitis (CASP), a model polymicrobial sepsis. Methods For clodronate liposomes were injected 24 h before CASP surgery female C57BL/6N mice. Three 12 after CASP, in-vivo fluorescence microscopy liver was performed. Analysis...
The particular importance of the vagus nerve for pathophysiology peritonitis becomes more and apparent. In this work we provide evidence vagal modulation inflammation in murine model colon ascendens stent (CASP). Vagotomy significantly increases mortality polymicrobial sepsis. This effect is not accounted by dilatation gastric volume following vagotomy. As stimulation cholinergic receptors nicotine has no therapeutic effect, lack also reason reduced survival rate. fact, increased septic a...
Successful treatment of severe sepsis and septic shock remains a major challenge in critical care medicine. The recently introduced recombinant human activated protein C (APC) remarkably improved the outcome patients. influence APC on intestinal circulation is still poorly understood. Therefore, present study aimed to investigate effects microcirculation during experimental endotoxaemia rats by using intravital microscopy. A total 44 male Lewis were randomly assigned receive intravenous...
To investigate the role of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) in postoperative polymicrobial abdominal sepsis.Sepsis is leading cause death among critically ill surgical patients. TRAIL commonly known as an agent cancer cells. It also plays important regulation inflammatory reactions. The sepsis still unclear.Experimental animal model.University laboratory.C57BL/6 mice.Colon ascendens stent peritonitis (CASP) was induced female mice. One hour, 24 hrs, and...
Sepsis remains a persistent problem on intensive care units all over the world. Understanding complex mechanisms of sepsis is precondition for establishing new therapeutic approaches in this field. Therefore, animal models are required that able to closely mimic human disease and also sufficiently deal with scientific questions. The Colon Ascendens Stent Peritonitis (CASP) highly standardized model polymicrobial abdominal rodents. In model, small stent surgically inserted into ascending...
Abstract Background The prevention of nosocomial infections requires participation from the patients themselves. In past, however, have been apprehensive to point out hygiene-relevant behaviour personnel. project AHOI, possibilities active patient involvement in infection are identified, tested and realized. goal is a strategy based upon three dimensions: “adherence”, “empowerment” “acceptance”. “AHOI” stands for “ A ctivation patients, persons need care givers H ygiene-conscious participati...
Infections, in particular with multidrug-resistant organisms, are a burden for inpatient and outpatient care the whole community. The pathogens "roam" patients their relatives, forming an epidemiological bridge between different facilities. Patients could play important role infection control, given that they properly involved. AHOI project stands Activation of patients, people need care, care-providers Hygiene-conscious participatiOn Infection prevention. To this end, multimodal...
Apart from inducing apoptosis in tumor cells, necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) influences inflammatory reactions. Murine colon ascendens stent peritonitis (CASP) represents a model of diffuse peritonitis. Recently, it has been demonstrated that administration exogenous TRAIL not only induces neutrophils but also enhances survival this model. The aim study was to examine the impact genetic deficiency on course CASP.Peritonitis induced 6- 8-week-old female (-/-)...
Aim: Since the introduction of surgical gloves, one main challenges has been to improve donning and wearability. For wearer, formation "glove juice" is problematic. To gliding properties for gloves absorbing sweat, in 1963 Bio-sorb® cream: sterile powder cream (Johnson & Johnson Medical, Gargrave, Skipton, United Kingdom) was introduced. Individuals subjectively reported a reduction sweat production inside glove, however, actual effect on accumulation hand antisepsis not examined. Methods:...
Postoperatively acquired immune dysfunction is associated with a higher mortality rate in case of septic complications. As details this severe clinical problem are still unknown, animal models essential to characterise the mechanisms involved.Mice were laparotomised and small intestine was pressed smoothly antegrade direction. For extension trauma, manipulated three times consecutively. Following this, ex vivo cytokine release splenocytes determined. The degree surgical trauma analysed by...
<b><i>Background:</i></b> Abdominal surgery is frequently followed by immune dysfunction usually lasting for several days. This especially important in cases with tumour diseases as an intact function essential this situation. Therefore, we analysed the outcome of tumour-bearing mice a mouse model surgically induced (SID). <b><i>Methods:</i></b> In male C57BL/6 mice, pancreatic was implanted orthotopically. Following implantation, SID applied....