Abstract Metastatic colorectal cancer is the fourth most common cause of death. Current options in palliation such as hyperthermic intraperitoneal chemotherapy (HIPEC) present severe side effects. Recent research efforts suggested therapeutic use oxidant-enriched liquid using cold physical plasma. To investigate a clinically accepted treatment regimen, we assessed antitumor capacity plasma-treated saline solution. In response to liquid, CT26 murine colon cells were readily oxidized and...

Medical technologies from physics are imperative in the diagnosis and therapy of many types diseases. In 2013, a novel cold physical plasma treatment concept was accredited for clinical therapy. This gas jet technology generates large amounts different reactive oxygen nitrogen species (ROS). Using melanoma model, is tested as anticancer agent. Plasma derived ROS diminish tumor growth vitro vivo. Varying feed mixture modifies composition ROS. Conditions rich atomic correlate with killing...

Reactive oxygen species (ROS/RNS) are produced during inflammation and elicit protein modifications, but the immunological consequences largely unknown. Gas plasma technology capable of generating an unmatched variety ROS/RNS is deployed to mimic study significance modifications using model chicken ovalbumin (Ova vs oxOva). Dynamic light scattering circular dichroism spectroscopy reveal structural in oxOva compared Ova. T cells from Ova-specific OT-II not C57BL/6 or SKH-1 wild type mice...

Gas plasma technology generates reactive oxygen and nitrogen species (ROS/RNS), inducing lethal oxidative damage in tumor cells. The transfer of gas plasma–derived ROS/RNS into liquids has been proposed as an innovative anti-cancer strategy targeting peritoneal carcinomatosis (PC). However, the mechanism action is under debate. To this end, we compared plasma–oxidized medical-grade sodium chloride (oxNaCl) with a concentration-matched control (cmc) NaCl enriched equivalent concentrations H 2...

Laminin (A:B1:B2) is a major component of the first basement membrane to appear in developing mouse embryo. Its effects on morphogenesis and differentiation are mediated by interaction with cell surface receptors that members integrin family. We have studied expression alpha 6 subunit murine beta 1 its ligand, laminin, preimplantation embryos, embryo outgrowths embryonic stem (ES) cells embryonal carcinoma (EC) cells. The present oocyte throughout development. A chain appears later than has...

While many new and emerging therapeutic concepts have appeared throughout the last decades, cancer still is fatal in patients. At same time, importance of immunology oncotherapy increasingly recognized, not only since advent checkpoint therapy. Among types tumors, also breast has an immunological dimension that might be exploited best by increasing immunogenicity tumors microenvironment. To this end, we tested a novel concept, gas plasma irradiation, for its ability to promote increase...

Cold physical plasma has limited tumor growth in many preclinical models and is, therefore, suggested as a putative therapeutic option against cancer. Yet, studies investigating the cells' metastatic behavior following treatment are scarce, although being of prime importance to evaluate safety this technology. Therefore, we investigated four human pancreatic cancer cell lines for their vitro chicken embryos (in ovo). Pancreatic was chosen it is particularly peritoneum systemically, which...

Cold plasma technology is an emerging tool facilitating the spatially controlled delivery of a multitude reactive species (ROS) to skin. While therapeutic efficacy treatment has been observed in several types diseases, fundamental consequences plasma-derived ROS on skin physiology remain unknown. We aimed bridge this gap since epidermal barrier and perfusion plays vital role health disease by maintaining homeostasis protecting from environmental damage. The intact SKH1 mice was...

In a search for functions of transforming growth factor-beta during early embryonic development we used two different experimental approaches. the first made use stem (ES) cells. ES cells in culture differentiate to derivatives all three germ layers and mimic some aspects organogenesis when grown as aggregates suspension form embryoid bodies. Differentiation proceeds further bodies attach suitable substrates. Muscle neuronal are among most readily identified cell types then formed. We...

Poor prognosis of pancreatic cancer has remained almost unchanged in recent years. Cold physical plasma was suggested as an innovative anticancer strategy, but its selective killing activity malignant over non-malignant cells only partially been explored. The present study aimed at exploring the effect cold on cellular viability.Induction cell death and apoptosis by investigated murine PDA6606 primary fibroblasts vitro (2D 3D cultures) ovo.Plasma increased to a significantly higher extent...

Tumour-associated macrophages (TAMs) are highjacked M2-polarized that especially promote pancreatic cancer growth. The aim of this study was to identify an easy-to-use cell culture model suitable for studying interaction and macrophage polarization.Co-cultures two lines, PDA6606 cells with RAW were used in vitro ovo. Macrophages analyzed by microscopy, magnetic resonance imaging (MRI), flow cytometry.By comparing chemically-induced M1 M2 macrophages, a clear induction the phenotype observed...

Pancreatic cancer is one of the most aggressive tumor entities. Diffuse metastatic infiltration vessels and peritoneum restricts curative surgery. Standard chemotherapy protocols include cytostatic drug gemcitabine with limited efficacy at considerable toxicity. In search a more effective less toxic treatment modality, we tested in human pancreatic cells (MiaPaca PaTuS) novel combination therapy consisting drugs (gemcitabine or cisplatin) gas plasma-conditioned Ringer's lactate that acts via...

Despite continuous advances in therapy, malignant melanoma is still among the deadliest types of cancer. At same time, owing to its high plasticity and immunogenicity, regarded as a model tumor entity when testing new treatment approaches. Cold physical plasma novel anticancer tool that utilizes plethora reactive oxygen species (ROS) being deposited on target cells tissues. To test whether would enhance toxicity an established antitumor ionizing radiation, we combined both modalities...

Abstract Strategies to improve activity and selectivity are major goals in oncological drug development. Medical gas plasma therapy has been subject intense research dermatooncology recently. Based on partial ionization, this approach is exceptional generating a variety of reactive oxygen species simultaneously that can be applied locally at the tumor side. It hypothesized combined treatment potentiate responses melanoma. Using jet approved as medical device Europe, systematic screening 46...

Colorectal cancer metastasis often occurs before clinical diagnosis and leads to diffuse spread of tumor nodules in the peritoneal cavity carcinomatosis. These small lesions are difficult remove surgically poor prognosis. Current options palliation include ionizing radiation hyperthermic intraperitoneal chemotherapy that lead severe side effects. In previous research, oxidant-enriched solutions, like physical plasma-treated cell culture medium, showed therapeutic potential diminishing growth...

Gas plasmas, often referred to as cold physical plasma, are currently being investigated for their potential serve anticancer agents. Along similar lines, gas plasma-oxidized liquids a carrier reactive oxygen species have found way into preclinical research. This review focuses on <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">in vivo</i> studies that utilized such cancer therapies. These tumor models, treatment modalities, and types of were...

Abstract Recent research indicated the potential of cold physical plasma in cancer therapy. The plethora plasma-derived reactive oxygen and nitrogen species (ROS/RNS) mediate diverse antitumor effects after eliciting oxidative stress cells. We aimed at exploiting this principle using a newly designed dual-jet neon source ( V jet) to treat colorectal A treatment time-dependent ROS/RNS generation induced oxidation, growth retardation, cell death within 3D tumor spheroids were found. In...

Abstract The α6β1 integrin is a receptor for laminins and present from early stages of mouse embryogenesis. In the study determined temporal spatial expression two cytoplasmic splice variants α6 subunit, α6A α6B, in early‐ midgestation postimplantation embryo using RT‐PCR, situ hybridization, immunofluorescence. Our results show that α6B at all studied expressed before α6A. begins 8.5 day p.c. embryos initially exclusively localized to developing heart. (and 9.5) mRNA protein are gradient...

Increasing numbers of cancer deaths worldwide demand for new treatment avenues. Cold physical plasma is a partially ionized gas expelling variety reactive oxygen and nitrogen species, which can be harnesses therapeutically. Plasmas plasma-treated liquids have antitumor properties in vitro vivo. Yet, global response signatures to not yet been identified. To this end, we screened eight human cell lines investigate effects low-dose, tumor-static medium (PTM) on cellular activity,...

Background: Monocyte-derived macrophages are key regulators and producers of reactive oxygen nitrogen species (ROS/RNS). Pre-clinical clinical studies suggest that cold physical plasma may be beneficial in the treatment inflammatory conditions via release ROS/RNS. However, it is unknown how affects monocytes their differentiation profile. Methods: Naïve or phorbol-12-myristate-13-acetate (PMA)-pulsed THP-1 were exposed to plasma. The cells analyzed regarding metabolic activity as well flow...