A5072235617- Mast cells and histamine
- Bacterial Identification and Susceptibility Testing
- Sepsis Diagnosis and Treatment
- Food Allergy and Anaphylaxis Research
- Streptococcal Infections and Treatments
- Clinical Laboratory Practices and Quality Control
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Antibiotic Use and Resistance
- COVID-19 Clinical Research Studies
- Neonatal Respiratory Health Research
- Eosinophilic Disorders and Syndromes
- Clostridium difficile and Clostridium perfringens research
- Allergic Rhinitis and Sensitization
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Nosocomial Infections in ICU
- Parvovirus B19 Infection Studies
- Antimicrobial Resistance in Staphylococcus
- Asthma and respiratory diseases
- Stress Responses and Cortisol
- Infective Endocarditis Diagnosis and Management
- Pediatric health and respiratory diseases
- Vitamin C and Antioxidants Research
- Click Chemistry and Applications
Medical University of Vienna
2012-2025
University of Alberta
2024
Ludwig Boltzmann Institute Applied Diagnostics
2020-2022
Klinikum rechts der Isar
1985
Advanced macrolides, such as azithromycin (AZM) or clarithromycin (CLM), are antibiotics with immunomodulatory properties. Here we have sought to evaluate their in vitro influence on the activation of CD4+ T-cells. Isolated T-cells were stimulated agonistic anti-CD3/anti-CD28 monoclonal antibodies presence 0.6 mg/L, 2.5 10 mg/L 40 AZM CLM. Cell proliferation, cytokine level supernatants and cell viability was assessed. Intracellular signaling pathways evaluated using reporter lines, FACS...
Physicians are regularly faced with severely ill patients at risk of developing infections. In literature, standard care wards often neglected, although their frequently suffer from a systemic inflammatory response syndrome (SIRS) unknown origin. Fast identification infections is vital, as they immediately require appropriate therapy. Further, tools high negative predictive value (NPV) to exclude infection or bacteremia important increase the cost effectiveness microbiological examinations...
The analytically sensitive detection of KIT D816V in blood and bone marrow is important for diagnosing systemic mastocytosis (SM). Additionally, precise quantification the variant allele fraction (VAF) relevant clinically because it helps to predict multilineage involvement prognosis cases advanced SM. Digital PCR (dPCR) a promising new method accurate somatic mutations.We performed validation study dPCR on 302 peripheral samples from 156 patients with comparison melting curve analysis after...
Non-vitamin K antagonist oral anticoagulants (NOAC), including rivaroxaban, apixaban or dabigatran, regularly show relevant effects on coagulation tests, making the interpretation of results difficult. The aim this study was to evaluate possible interferences NOACs in trough level concentrations lupus anticoagulant (LA) testing. Citrate plasma specimens 30 healthy volunteers were spiked with dabigatran four concentration levels at below NOAC levels. measured using dedicated surrogate tests...
Abstract Chloroquine (CQ) is widely used as an anti-inflammatory therapeutic for rheumatic diseases. Although its modes of action on the innate immune system are well described, there still insufficient knowledge about direct effects adaptive system. Thus, we evaluated influence CQ activation parameters human CD4 + T-cells. directly suppressed proliferation, metabolic activity and cytokine secretion T-cells following anti-CD3/anti-CD28 activation. In contrast, showed no effect up-regulation...
Bacteraemia is a life-threating condition requiring immediate diagnostic and therapeutic actions. Blood culture (BC) analyses often result in low true positive rate, indicating its improper usage. A predictive model might assist clinicians deciding for whom to conduct or avoid BC analysis patients having relevant bacteraemia risk. Predictive models were established by using linear non-linear machine learning methods. To obtain proper data, unique data set was collected prior estimation...
Burnout and work-related stress symptoms of anxiety disorder depression cause prolonged work absenteeism early retirement. Hence, reliable identification patients under risk monitoring treatment success is highly warranted. We aimed to evaluate stress-specific biomarkers in a population-based, "real-world" cohort (burnouts: n = 40, healthy controls: 26), recruited at preventive care ward, baseline after four-month follow up, during which received medical psychological treatment. At baseline,...
Abstract Tryptophan metabolites, including kynurenine, 3-hydroxyanthranilic acid, and picolinic are key mediators of immunosuppression by cells expressing the tryptophan-catabolizing enzyme indoleamine2,3-dioxygenase. In this study, we assessed influence acid on cell viability effector functions CD4+ T following in vitro activation with agonistic anti-CD3/anti-CD28 antibodies. contrast to kynurenine exposure did not affect viability, whereas proliferation metabolic activity were suppressed a...
A high allele burden of the KIT D816V mutation in peripheral blood or bone marrow aspirates indicates multi-lineage hematopoietic involvement and has been associated with an aggressive clinical course systemic mastocytosis. Since mast cells are substantially underrepresented these liquid specimens, their likely underestimates tumor disease. We used a novel previously validated digital polymerase chain reaction (PCR) method for analysis to systematically analyze formalin-fixed,...
Background Bacteraemia is a frequent and severe condition with high mortality rate. Despite profound knowledge about the pre-test probability of bacteraemia, blood culture analysis often results in low rates pathogen detection therefore increasing diagnostic costs. To improve cost-effectiveness sampling, we computed risk prediction model based on highly standardizable variables, ultimate goal to identify via an automated decision support tool patients very for bacteraemia. Methods In this...
Abstract The clinical role of vitamin D in sepsis and mortality prediction is controversially discussed. Therefore, we conducted a prospective cohort study on standard care wards, including 461 patients with suspected fulfilling two or more SIRS criteria. On the first third day after onset symptoms levels 25(OH)D, 1,25(OH)D biomarkers were analysed for their predictive capacity identifying infection, bacteraemia an elevated risk. Additionally, several SNPs associated metabolism evaluated....
Abstract Background Neutropaenic patients are at a high risk of contracting severe infections. In particular, in these patients, parameters with negative predictive value desirable for excluding infection or bacteraemia. This study evaluated sepsis biomarkers neutropaenic suffering from systemic inflammatory response syndrome ( SIRS ). Further, the capacities were compared to non‐neutropaenic patients. Material and methods this prospective observational cohort study, clinically suspected...
Abstract This study analyzed the performance of different molecular technologies along with blood culture (BC) in diagnosis bloodstream infections (BSI) patients from internal medicine wards - including intensive care units (ICUs) and emergency room. Patients systemic inflammatory response syndrome were prospectively included. BCs EDTA whole obtained simultaneously. The latter was by PCR combined electrospray ionization mass spectrometry (PCR/ESI-MS; IRIDICA BAC BSI assay, Abbott) SeptiFast...
Secondary hemophagocytic lymphohistiocytosis (sHLH) is a life-threatening condition clinically presenting as SIRS (Systemic Inflammatory Response Syndrome). However, there no comprehensive data concerning diagnostic algorithms, prevalence, outcome and biomarker performance in patients. We conducted prospective observational cohort study on 451 consecutive patients fulfilling ≥2 criteria. The Hscore the HLH-2004 criteria were used to determine presence of sHLH, correlation...
Determination of antibody levels against the nucleocapsid (N) and spike (S) proteins severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are used to estimate humoral immune response after SARS-CoV-2 infection or vaccination. Differences in design specification assays challenge interpretation test results, comparative studies often limited single time points per patient. We determined longitudinal kinetics 145 unvaccinated disease 2019 (COVID-19) patients at four visits over 1 year...
Childhood obesity is an increasing health care problem associated with insulin resistance and low-level systemic inflammation, which can ultimately lead to diabetes. Evidence for efficacy of therapeutic intervention programs on the early development sequelae moderate. This paper investigates effect a multidisciplinary short-term program metaflammation in childhood obesity. Two hundred 36 overweight or obese children adolescents between ages 10 14 were included prospective 5 months study,...
Background and aims: Platelet function testing may help to identify poor responders antiplatelet drugs. The aim of this study was compare three commonly used platelet tests with special focus on the pre-analytical influence time-delay tested parameters.Methods: We assessed ADP-induced by Multiplate, Function Analyzer-100 (PFA-100) VerifyNow in nine healthy volunteers 36 patients receiving clopidogrel or prasugrel 1 3 hours after sampling. Results: PFA-100 demonstrated non-closure time 23...
The immediate need for appropriate antimicrobial therapy in septic patients requires the detection of causative pathogen a timely and reliable manner. In this study, real-time PCR Septifast MGrade test was evaluated adult meeting systemic inflammatory response syndrome (SIRS) criteria that were treated at standard care wards.Patients with clinical suspected infection, drawn blood cultures (BC), M(Grade) (SF) sepsis biomarkers prospectively screened fulfillment SIRS using European Centre...
Background and Aim Recent studies revealed a link between hypovitaminosis D3 the risk for hyperglycemia. Further mechanistic interventional investigations suggested common reason both conditions rather than causal relationship. Exposure to sunlight is most relevant source of vitamin (25(OH)D), whereas adipose tissue able store amounts lipophilic vitamin. Since running/bicycling leads increased out-door time alters physiological response mechanisms, it can be hypothesized that correlation...
Background A major obstacle to effectively treat and control tuberculosis is the absence of an accurate, rapid, low-cost diagnostic tool. new approach for screening patients use rapid classification algorithms. Methods We tested a previously published algorithm based on four biomarkers as tool in Central European patient population using assessor-blinded cross-sectional study design. In addition, we developed improved at tertiary hospital Vienna, Austria, by supervised computational...
Background: Automated analyzers are an important component of modern laboratories. As a representative the newest generation coagulation analyzers, CS-5100 features several technical refinements including pre-analytical assessment unit as well multi-wavelength optical detection units. Therefore, is supposed to rapidly and accurately perform broad panel tests. In current study, was evaluated regarding its precision practicability in clinical laboratory setting.
Anemia in chronic kidney disease (CKD) is an almost universal complication of this condition. Fibroblast growth factor 23 (FGF23), a key-player mineral metabolism, reportedly associated with anemia and hemoglobin levels non-dialysis CKD patients. Here, we sought to further characterize association while taking into account the biologically active, intact fraction FGF23, iron erythropoietin (EPO). Hemoglobin, EPO, iron, metabolism parameters, including both c-terminal-FGF23 (iFGF23 cFGF23,...