A5040679586- Extracellular vesicles in disease
- Mesenchymal stem cell research
- IL-33, ST2, and ILC Pathways
- Asthma and respiratory diseases
- MicroRNA in disease regulation
- Neonatal Respiratory Health Research
- Allergic Rhinitis and Sensitization
- Eosinophilic Esophagitis
- Tissue Engineering and Regenerative Medicine
- Sinusitis and nasal conditions
- Glaucoma and retinal disorders
- Neurogenesis and neuroplasticity mechanisms
- Cancer-related molecular mechanisms research
- Retinal Development and Disorders
- RNA Interference and Gene Delivery
- Nerve injury and regeneration
- Axon Guidance and Neuronal Signaling
- Head and Neck Surgical Oncology
- Pediatric health and respiratory diseases
- Air Quality and Health Impacts
- Circular RNAs in diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Nasal Surgery and Airway Studies
- Cancer Cells and Metastasis
- Retinal Diseases and Treatments
Shanghai Eye Disease Prevention & Treatment Center
2025
The First Affiliated Hospital, Sun Yat-sen University
2015-2024
Sun Yat-sen University
2015-2024
Huazhong Agricultural University
2022
Culture Resource
2021
Chinese University of Hong Kong
2007-2019
University of Hong Kong
2007-2019
First People's Hospital of Foshan
2016
Changhai Hospital
2013
Second Military Medical University
2013
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of cell origin and change functions phenotypes other cells. These features indicate strong biomarker therapeutic potential have generated broad interest, as evidenced by steady year-on-year increase in numbers scientific publications about EVs. Important advances been made EV metrology understanding applying biology. However, hurdles remain to realising EVs domains ranging from basic biology clinical...
Abstract Background Chronic sinusitis (CRS) is a common otorhinolaryngologic disease that frequently encountered in everyday practice, but there lack of precise data regarding the prevalence CRS developing countries. We performed national investigation China to determine and associated factors CRS. Methods conducted cross‐sectional 2012. A stratified four‐stage sampling method was used select participants randomly from seven cities mainland China. All were interviewed face‐to‐face via...
Group 2 innate lymphoid cells (ILC2s) are recently reported to play a more critical role in allergic diseases. We previously identified that mesenchymal stromal (MSCs) elicited therapeutic effects on airway inflammation. Small extracellular vesicles (sEV) derived from MSCs possess striking advantages including low immunogenicity and high biosafety, is extremely promising cell-free agents. However, the of MSC-sEV ILC2s still unclear. Additionally, scalable isolation protocols required for...
We previously identified an immunomodulatory role of human induced pluripotent stem cell (iPSC)-derived mesenchymal cells (MSCs) in asthmatic inflammation. Mitochondrial transfer from bone marrow MSCs to epithelial can result the attenuation acute lung injury mice. However, effects mitochondrial iPSC-MSCs asthma and mechanisms underlying these are unclear. found that iPSC-MSC transplantation significantly reduced T helper 2 cytokines, attenuated dysfunction cells, alleviated inflammation...
We previously found that mesenchymal stem cells (MSCs) derived from human-induced pluripotent (iPSCs) exerted immunomodulatory effects on Th2-mediated allergic rhinitis in vitro. However, their contribution to the asthma and animal models remains unclear. In this study, we developed a mouse model of ovalbumin (OVA)-induced inflammation both upper lower airways evaluated systemic administration human iPSC-MSCs bone marrow-derived MSCs (BM-MSCs) inflammation. Our results showed treatments with...
Human induced pluripotent stem cells (iPSCs) possess remarkable self-renewal capacity and the potential to differentiate into novel cell types, such as mesenchymal (MSCs). iPSC-MSCs have been shown enhance tissue regeneration attenuate ischaemia; however, their contribution immune regulation of Th2-skewed allergic rhinitis (AR) asthma remains unclear.This study compared immunomodulatory effects bone marrow-derived MSCs (BM-MSCs) on lymphocyte proliferation, T-cell phenotypes cytokine...
Abstract Transplantation of bone marrow mesenchymal stem cells (BM-MSCs) can protect cardiomyocytes against anthracycline-induced cardiomyopathy (AIC) through paracrine effects. Nonetheless the effects human induced pluripotent cell-derived MSCs (iPSC-MSCs) on AIC are poorly understood. In vitro studies reveal that doxorubicin (Dox)-induced reactive oxidative stress (ROS) generation and cell apoptosis in neonatal rat (NRCMs) significantly reduced when treated with conditioned medium...
Rationale: Retinal ganglion cell (RGC) degeneration is extremely hard to repair or regenerate and often coupled with mitochondrial dysfunction. Mesenchymal stem cells (MSCs)-based treatment has been demonstrated beneficial for RGC against degeneration. However, underlying mechanisms of MSC-provided protection are largely unknown other than neuroprotective paracrine actions. In this study, we sought investigate whether donation from induced pluripotent cell-derived MSC (iPSC-MSCs) could...
Mesenchymal stem cells (MSCs) have potent immunomodulatory effects on multiple immune and great potential in treating disorders. Induced pluripotent (iPSCs) serve as an unlimited noninvasive source of MSCs, iPSC-MSCs been reported to more advantages exhibit immunomodulation T lymphocytes natural killer cells. However, the dendritic (DCs) are unclear. The aim this study is investigate differentiation, maturation, function DCs. Human monocyte-derived DCs were induced cultured presence or...
Group 2 innate lymphoid cells (ILC2s) were closely associated with asthma. However, there no perspective studies about the effects of glucocorticoid on ILC2s in asthma patients. Our objective was to perform a study and evaluate ILC2 activity after therapy patients.The allergic rhinitis patients treated for 3 months. The circulating levels evaluated. possible signalling pathways investigated vitro.The well-controlled, high significantly decreased at 1 months treatment. Peripheral blood...
Mesenchymal stem cell- (MSC-) based therapy is a novel strategy in regenerative medicine. The functional and capacities of MSCs decline with senescence. Nonetheless, the potential mechanisms that underlie their senescence are not fully understood. This study was aimed at exploring fibroblast growth factor 21 (FGF21) regulation MSC determined by senescence-associated β -galactosidase (SA- -gal) staining. morphology level mitochondrial reactive oxygen species (ROS) were assessed MitoTracker...
BackgroundBone destruction is one of many severe complications that occurs in patients with rheumatoid arthritis (RA) and current therapies are unable to cure this manifestation. This study here aims determine whether GMSC can directly inhibit osteoclast formation eventually attenuate osteoclastogenesis bone erosion an inflammatory milieu.MethodGMSC were co-cultured precursors or without CD39 inhibitor, CD73 inhibitor adenosine receptors inhibitors pretreatment evaluated vitro. 2×10^6 per...
Application of mesenchymal stem cell-derived exosomes (MSC-EXO) has emerged as a novel therapeutic strategy for myocardial infarction (MI). Our previous study showed that pretreatment with hemin, potent heme oxygenase-1 (HO-1) inducer, enhanced the cardioprotective effects MSCs in mouse model MI. This aimed to investigate EXO derived from hemin-pretreated (Hemin-MSC-EXO) MI and explore potential mechanisms.MSC-EXO Hemin-MSC-EXO were collected characterized. MSC-EXO intramuscularly injected...
Mesenchymal stem cells (MSCs) have demonstrated potent immunomodulatory properties that shown promise in the treatment of autoimmune diseases, including rheumatoid arthritis (RA). However, inherent heterogeneity MSCs triggered conflicting therapeutic outcomes, raising safety concerns and limiting their clinical application. This study aimed to investigate potential extracellular vesicles derived from human gingival mesenchymal (GMSC-EVs) as a strategy for RA. Through vivo experiments using...
Abstract Adult mesenchymal stem cells (MSCs) are immunoprivileged due to the low expression of major histocompatibility complex (MHC) II molecules. However, MHC molecules in human-induced pluripotent (iPSCs)-derived MSCs has not been investigated. Here, we examined human leukocyte antigen (HLA) derived from iPSCs, fetuses, and adult bone marrow (BM) after stimulation with interferon-γ (IFN-γ), compared their repair efficacy, cell retention, inflammation, HLA immune humanized NOD Scid gamma...
Abstract Therapeutic applications of tissue-derived mesenchymal stem cells (MSCs) are hindered by their limited expansion ability and variation across donors. Human induced pluripotent cell (iPSC)-derived MSCs show greater expandability therefore offer potential for use in tissue repair therapies. Here we explored the regenerative effects iPSC-MSCs mechanisms which promote mucosal healing via tumor necrosis factor-α-stimulated gene 6 (TSG-6) mouse models inflammatory bowel disease (IBD)....
Group 2 innate lymphoid cells (ILC2s) are essential in initiating and driving allergic immune responses. However, there were inconsistent findings of the ILC2 levels rhinitis (AR) patients. This study investigated peripheral blood house dust mite (HDM)-sensitized AR patients their ability to secrete type cytokines. The ILC2s with phenotypic characteristics increased HDM-AR patients' symptom score IL-13 positively associated epithelial cytokine stimulation induced dramatic production IL-5...
Allergic airway inflammation is a major public health disease that affects up to 300 million people in the world. However, its management remains largely unsatisfactory. The dysfunction of pulmonary macrophages contributes greatly development allergic inflammation. It has been reported small extracellular vesicles derived from mesenchymal stromal cells (MSC-sEV) were able display extensive therapeutic effects some immune diseases. This study aimed investigate MSC-sEV on inflammation, and...
Mesenchymal stromal cells (MSCs) are well known for their immunoregulatory roles on allergic inflammation particularly by acting T cells, B and dendritic (DCs). MSC-derived small extracellular vesicles (MSC-sEV) increasingly considered as one of the main factors effects MSCs immune responses. However, MSC-sEV DCs in diseases remain unclear. were prepared from induced pluripotent stem (iPSC)-MSCs anion-exchange chromatography, characterized with size, morphology, specific markers. Human...
Abstract “Requirements for human mesenchymal stem cell‐derived small extracellular vesicles” is the first set of guidelines on vesicles (hMSC‐sEVs) in China, jointly drafted and agreed upon by experts from Chinese Society Extracellular Vesicles. It was released Research Hospital Association 28 December 2021 came into effect 1 January 2022. This standard specifies technical requirements, test methods regulations, packaging, instructions use, labeling, storage, transportation, waste handling...
Abstract Senescence of vascular smooth muscle cells (VSMCs) contributes to the formation abdominal aortic aneurysm (AAA). Although mesenchymal stem cell exosomes (MSC-EXO) have been confirmed restrict development AAA, their biological activity depends largely on physiological state MSCs. This study aimed compare effects adipose-derived MSC-EXO from healthy donors (HMEXO) and AAA patients (AMEXO) senescence VSMCs in explore underlying mechanisms. An ApoE-/- mouse model was used investigate...