A5058801737- Connective tissue disorders research
- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Gene expression and cancer classification
- HER2/EGFR in Cancer Research
- Bone fractures and treatments
- Osteoarthritis Treatment and Mechanisms
- Computational Drug Discovery Methods
- Bone health and treatments
- RNA Research and Splicing
- Synthesis and biological activity
- COVID-19 Impact on Reproduction
- Advanced Breast Cancer Therapies
- Bioinformatics and Genomic Networks
- Genomics and Chromatin Dynamics
- Bone Metabolism and Diseases
- Genomic variations and chromosomal abnormalities
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Electrocatalysts for Energy Conversion
- Genetic Associations and Epidemiology
- Neuroinflammation and Neurodegeneration Mechanisms
- COVID-19 Clinical Research Studies
- Protease and Inhibitor Mechanisms
- Spinal Cord Injury Research
University of Hong Kong - Shenzhen Hospital
2021-2025
University of Hong Kong
2012-2025
Weatherford College
2023
Chinese University of Hong Kong
2018-2022
Peking University
2016
Methodist Hospital
2013
The University of Texas at Austin
2013
Cornell University
2013
Children's Cancer Center
2013
Baylor College of Medicine
2013
Subtypes are widely found in cancer. They characterized with different behaviors clinical and molecular profiles, such as survival rates, gene signature copy number aberrations (CNAs). While cancer is generally believed to have been caused by genetic aberrations, the of events tremendous tissue only a small subset them may be tumorigenic. On other hand, expression subtype represents residuals subtype-specific mechanisms. Using high-throughput data link these factors define boundaries...
Pt‐based electrocatalysts for the oxygen reduction reaction (ORR) are topic of extensive and intensive research since a few decades. Nevertheless, scarcity these electrocatalysts, their high cost unsatisfactory durability primary hindrances to further commercialization. In recent years, non‐Pt have garnered considerable interest as alternatives catalysts ORR. This review highlights synthesis, catalytic activity key factors, namely also active sites, various nanostructured that can be grouped...
Intervertebral disc degeneration (IVD) is a leading cause of low back pain, prevalent musculoskeletal condition. IVD characterized by the degradation nucleus pulposus (NP), annulus fibrosus (AF), and cartilage endplates (EP). Growth Differentiation Factor 6 (GDF6), part bone morphogenetic protein family, has demonstrated potential in maintaining integrity. However, its precise role cellular synthesis during remains unclear. This study employed Stable Isotope Labeling Amino Acids Cell Culture...
Rationale: Retinal ganglion cell (RGC) degeneration is extremely hard to repair or regenerate and often coupled with mitochondrial dysfunction. Mesenchymal stem cells (MSCs)-based treatment has been demonstrated beneficial for RGC against degeneration. However, underlying mechanisms of MSC-provided protection are largely unknown other than neuroprotective paracrine actions. In this study, we sought investigate whether donation from induced pluripotent cell-derived MSC (iPSC-MSCs) could...
Intervertebral disc degeneration might be amenable to stem cell therapy, but the required cells are scarce. Here, we report development of a protocol for directed in vitro differentiation human pluripotent (hPSCs) into notochord-like and nucleus pulposus (NP)-like disc. The first step combines enhancement ACTIVIN/NODAL WNT inhibition BMP pathways. By day 5 differentiation, hPSC-derived express notochordal characteristic genes. After activating TGF-β pathway an additional 15 days, qPCR,...
The spatiotemporal proteome of the intervertebral disc (IVD) underpins its integrity and function. We present DIPPER, a deep comprehensive IVD proteomic resource comprising 94 genome-wide profiles from 17 individuals. To begin with, protein modules defining key directional trends spanning lateral anteroposterior axes were derived high-resolution spatial proteomes intact young cadaveric lumbar IVDs. They revealed novel region-specific regulatory activities displayed potential paths...
The synovial joint forms from a pool of progenitor cells in the future region joint, interzone. Expression Gdf5 and Wnt9a has been used to mark earliest cellular processes formation interzone cells. However, lineage specification progression toward different tissues are not well understood. Here, by lineage-tracing studies we identify population Lgr5+ that contribute cruciate ligaments, membrane, articular chondrocytes joint. This finding is supported single-cell transcriptome analyses. We...
Abstract Backgrounds Intracranial germ cell tumors (GCTs) are rare and heterogeneous with very little is known about their pathogenesis underlying genetic abnormalities. Procedures In order to identify candidate genes pathways which involved in the of these tumors, we have profiled 62 intracranial GCTs for DNA copy number alterations (CNAs) loss heterozygosity (LOH) by using single nucleotide polymorphism (SNP) array quantitative real time PCR (qPCR). Results Initially 27 cases tumor tissues...
A new type of signaling network element, called cancer bridges (CSB), has been shown to have the potential for systematic and fast-tracked drug repositioning. On basis CSBs, we developed a computational model derive specific downstream pathways that reveal previously unknown target-disease connections mechanisms subtypes. The enables us reposition drugs based on available patient gene expression data. We applied this repurpose known or shelved brain, lung, bone metastases breast with...
Bietti's crystalline dystrophy (BCD) is an incurable retinal disorder caused by the polypeptide 2 of cytochrome P450 family 4 subfamily V (CYP4V2) mutations. Patients with BCD present degeneration pigmented epithelial (RPE) cells and consequent blindness. The lack appropriate disease models patients' RPE limits our understanding pathological mechanism degeneration. In this study, using CYP4V2 mutant pluripotent stem as models, we demonstrated that mutations presented a disrupted fatty acid...
The in vivo mechanisms underlying dominant syndromes caused by mutations SRY-Box Transcription Factor 9 ( SOX9 ) and SOX10 SOXE transcription factors, when they either are expressed alone or coexpressed, ill-defined. We created a mouse model for the campomelic dysplasia Y440X mutation, which truncates transactivation domain but leaves DNA binding dimerization intact. Here, we find that causes deafness via distinct endolymphatic sac (ES)/duct cochlea. By contrast, conditional heterozygous...
Osteogenesis imperfecta (OI) Type V is typically characterized by radial head dislocation, calcification of interosseous membrane and hyperplastic callus. It caused the c.-14C > T mutation in 5' UTR IFITM5 gene, adding five amino acids (MALEP) to N-terminal protein. Previous studies have suggested a neomorphic function MALEP-IFITM5 However, underlying mechanisms remain unclear due embryonic lethality previous mouse models. Therefore, we developed an inducible model (Ifitm5flox T) that could...
Bone homeostasis is regulated by hormones such as parathyroid hormone (PTH). While PTH can stimulate osteo-progenitor expansion and bone synthesis, how the PTH-signaling intensity in progenitors controlled unclear. Endochondral osteoblasts arise from perichondrium-derived osteoprogenitors hypertrophic chondrocytes (HC). We found, via single-cell transcriptomics, that HC-descendent cells activate membrane-type 1 metalloproteinase 14 (MMP14) pathway they transition to neonatal adult mice....
Glioblastoma Multiforme (GBM) cells are highly invasive, infiltrating into the surrounding normal brain tissue, making it impossible to completely eradicate GBM tumors by surgery or radiation. Increasing evidence also shows that these migratory resistant cytotoxic reagents, but decreasing their capability can re-sensitize them chemotherapy. These evidences suggest cell population may serve as a better therapeutic target for more effective treatment of GBM. In order understand regulatory...
Abstract Motivation It remains challenging to unravel new susceptibility genes of complex diseases and the mechanisms in genome-wide association studies. There are at least two difficulties, isolation genuine from many indirectly associated functional validation these genes. Results We first proposed a novel conditional gene-based test which can use only summary statistics isolate independently disease. Applying this method, we detected 185 independent with schizophrenia. then designed an...
• Proteomics of healthy mouse IVDs differentiating compartments and spine levels. NP cells feature vacuoles with lysosomal, transport cell–cell communication functions. Collagen XII, decorin other ECM proteins contribute to function the AF. Distinct proteomics between lumbar tail discs. Mouse is a relevant model for human disc biology but care needed in its use. Mice are commonly used study intervertebral (IVD) related diseases such as IVD degeneration. Discs from both regions used. However,...
Abstract The driver tissues or cell types in which susceptibility genes initiate diseases remain elusive. We develop a unified framework to detect the causal of complex traits according selective expression disease-associated genome-wide association studies (GWASs). This consists three components run iteratively produce converged prioritization list tissues. Additionally, this also outputs prioritized as byproduct. apply six representative with GWAS summary statistics, leads estimation lung...
Osteogenesis imperfecta (OI) is a rare inherited connective tissue dysplasia characterized with skeletal fragility, recurrent fractures and bone deformity, predominantly caused by mutations in the genes COL1A1 or COL1A2 that encode chains of type I collagen. In present study, clinical manifestations genetic variants were analysed from 187 Chinese OI patients, majority whom are southern origin. By targeted sequencing, 63 58 patients found carrying respectively, including 8 novel 7 variants....
Introduction: An increasing number of children with severe coronavirus disease 2019 (COVID-19) is being reported, yet the spectrum severity and expression patterns angiotensin-converting enzyme 2 (ACE2) in at different developmental stages are largely unknow.Methods: We analysed clinical features a cohort 173 between January 22, 2020 March 15, 2020.We systematically examined distribution ACE2 by using combination children's lung biopsies, pluripotent stem cell-derived cells, RNA-sequencing...
Extracellular matrices (ECMs) in the intervertebral disc (IVD), lung and artery are thought to undergo age-dependant accumulation of damage by chronic exposure mechanisms such as reactive oxygen species, proteases glycation. It is unknown whether this species-dependant (via differing lifespans hence cumulative exposures) or it can influence progression age-related diseases atherosclerosis. Peptide location fingerprinting (PLF) a new proteomic analysis method, capable non-targeted...
Abstract Background Osteogenesis imperfecta (OI) is a group of rare skeletal dysplasia. Long bone deformity and scoliosis are often associated with progressively deforming types OI. FKBP65 (encoded by FKBP10 , OMIM *607063) plays crucial role in the processing type I procollagen. Autosomal recessive variants result XI osteogenesis imperfecta. Methods Patients diagnosed OI were recruited for genetic test. RT‐PCR Sanger sequencing applied to confirm splicing defect mRNA splice‐site variant....
Silver nanoparticles (AgNPs) possess anti-inflammatory activities and have been widely deployed for promoting tissue repair. Here we explored the efficacy of AgNPs on functional recovery after spinal cord injury (SCI). Our data indicated that, in a SCI rat model, local delivery could significantly recover locomotor function exert neuroprotection through reducing pro-inflammatory M1 survival. Furthermore, comparison with Raw 264.7-derived M0 M2, higher level uptake more pronounced...