A5037362966- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
- COVID-19 Impact on Reproduction
- Neonatal Respiratory Health Research
- Influenza Virus Research Studies
- Respiratory viral infections research
- Cancer Cells and Metastasis
- CAR-T cell therapy research
- Autophagy in Disease and Therapy
- Phytochemistry and biological activity of medicinal plants
- SARS-CoV-2 and COVID-19 Research
- Remote Sensing and Land Use
- Cancer-related molecular mechanisms research
- FOXO transcription factor regulation
- Hops Chemistry and Applications
- Viral Infections and Vectors
- Layered Double Hydroxides Synthesis and Applications
- Data-Driven Disease Surveillance
- Long-Term Effects of COVID-19
- Nanoparticle-Based Drug Delivery
- Ferroptosis and cancer prognosis
- Polyoxometalates: Synthesis and Applications
The University of Queensland
2017-2021
Zhongshan Hospital
2018
Sun Yat-sen University
2016
Sun Yat-sen University Cancer Center
2016
Chinese Center For Disease Control and Prevention
2014
First Affiliated Hospital of Jinan University
2011
To determine if silencing PD-1 on tumor-infiltrating lymphocytes (TILs) and its ligand-1 (PD-L1) cancer cells will enhance the cytotoxicity of TILs.Lipid-coated calcium phosphate nanoparticles were synthesized to deliver siRNAs against PD-L1 TILs breast MCF-7 cells. The downregulation PD-1/PD-L1 expressions was determined by real-time PCR western blotting assays. killing efficacy cytotoxic T lymphocyte assay.Lipid-coated effectively delivered silenced PD-L1sh expression. knockdown either...
Cancer vaccines have attracted increasing attention for their application in tumor immunotherapy. DNA are one of them that been proven very promising with the advantages safety, rapid design, and low cost. However, stability, ineffective cell internalization, immunostimulation hinder wide application. Thus, developing targeted safe systems to effectively deliver becomes a vital step. In this study, we report development mannose- bisphosphonate (BP)-modified calcium phosphate (CP)...
Abstract Background Children and older adults with coronavirus disease 2019 (COVID-19) display a distinct spectrum of severity yet the risk factors aren’t well understood. We sought to examine expression pattern angiotensin-converting enzyme 2 (ACE2), cell-entry receptor for severe acute respiratory syndrome (SARS-CoV-2), role lung progenitor cells in children patients. Methods retrospectively analyzed clinical features cohort 299 patients COVID-19. The distribution ACE2 were systematically...
Previous studies reported that emodin extracted from Rheum palmatum L. exerts antiproliferation and antimetastatic effects in a variety of human cancer types. However, the role hepatocellular carcinoma (HCC) remain unknown.EdU colony formation assays were performed to evaluate on proliferation. The mobility capacities HCC treated with evaluated using wound healing assay. Transwell invasion migration anti-migratory anti-invasive HCC. Annexin V-FITC/PI was analyze apoptosis. PI stain cell...
Programmed cell death ligand 1 (PD-L1) is an important immune-inhibitory protein expressed on cancer cells to mediate escape through interaction with PD-1 activated T lymphocytes (T cells). Previously, we reported that colon and breast stem (CSCs) much higher levels of PD-L1 than their parental cells, suggesting they will be more resistant immune attack.We investigated the underlining mechanism increase in CSCs, a special focus effect insulin epithelial growth factor (EGF), two fundamental...
Suitable carriers are crucial to RNAi applications for cancer genotherapy and T-cell immunotherapy. In this research, we selected two extensively-investigated biocompatible inorganic nanoparticle carriers, i.e., layered double hydroxide (LDH) lipid-coated calcium phosphate (LCP) then compared their efficacy siRNA delivery in T cells, order understand which carrier is more efficient delivering functional programmed cell death protein 1 (PD-1 siRNA) suspended lymphocytes. Both LDH LCP...
Invasive bladder cancer is a lethal disease for which effective prognostic markers as well potential therapy targets are still lacking. Nkx2.8 (Nk2 homeobox 8), novel member of the NK-2 gene family, was reported to play an important role in development and progression human cancer. Herein, we that markedly reduced tissues compared with matched adjacent normal urothelial tissues. levels were inversely correlated advanced T classification, N tumor multiplicity, high proliferation index (Ki-67)...
The ATP-modified Calcium Phosphate (ACP) nano-adjuvant for DNA vaccines. ATP plays two key roles in this system: (1) a stabiliser CP to inhibit the particle size growth and aggregation, (2) immunological adjuvant vaccine.
Calcium phosphate (CP) nanoparticles (NPs) have been used in various applications for delivery of nucleic acid (NA) cargos with ideal biocompatibility and safety. However, some critical issues such as poor stability aggregation water solution hinder the industrial application CP NPs. To further utilize NPs NA delivery, this study specifically focused on modification to achieve a rapid synthesis improvement dispersibility colloidal by using bisphosphonate (BP) BSA (named BCP NPs). Compared...
Introduction: An increasing number of children with severe coronavirus disease 2019 (COVID-19) is being reported, yet the spectrum severity and expression patterns angiotensin-converting enzyme 2 (ACE2) in at different developmental stages are largely unknow.Methods: We analysed clinical features a cohort 173 between January 22, 2020 March 15, 2020.We systematically examined distribution ACE2 by using combination children's lung biopsies, pluripotent stem cell-derived cells, RNA-sequencing...
Layered double hydroxide (LDH) nanoparticles (NPs) are safe and effective vectors for small interfering RNA (siRNA) delivery. However, it is unclear whether there optimal parameters the efficient delivery of functional siRNA using LDH NPs. In this research, we comprehensively examined effect parameters, such as mixing method LDH/siRNA mass ratio on silencing capability. We first noted that best way loading gene segments (25 bp dsDNA siRNA) to add molecules 100 nm then diluting in Dulbecco's...
K-Ras is a well-studied oncogene, and its mutation frequently found in epithelial cancers like pancreas, lung, colorectal cancers. Cancer cells harboring mutations are difficult to treat due the drug resistance metastasis properties. stem (CSCs) believed major cause of chemotherapeutic responsible for tumor recurrence metastasis. But how affects CSCs inflammation not clear. Here, we compared two colon cancer cell lines, HCT-116 HT-29, with former being G13D mutant latter wildtype. We that...
Aim: Under suboptimal storage and transport conditions, influenza virus (flu-v) RNA is prone to degradation lysis buffers from extraction kits have a potential stabilize RNA. The aim of this study was investigate the effects different on stability flu-v Materials & methods: Aliquots suspension were processed in parallel with two buffers, then underwent cyclic freeze–thaw or prolonged at 4, 22 -20°C. viral analyzed by using real-time conventional RT-PCR amplifying, respectively, partial...
An increasing number of infected children are being encountered with the rapid spread COVID-19, yet spectrum disease severity and expression patterns angiotensin-converting enzyme 2 (ACE2), cell-entry receptor SARS-CoV-2 virus, in children's lungs at different developmental stages largely unknown. We retrospectively analysed clinical features laboratory parameters a cohort 234 (neonates to 16 yrs. old) COVID-19 infection. ACE2 distribution were examined by using combination human pluripotent...
As the current urban public security video surveillance system planning lacks of indicator and relies too much on subjective experience, in order to solve problem, this paper constructs evaluation indicators estimating number deployment front-end cameras, proposes methods transmission networks, data storage platform functions from macro micro levels by studying characteristic factors affecting frontend point location requirements various surveillant scenarios. This provides theoretical...
Background: An increasing number of children with severe coronavirus disease 2019 (COVID-19) is being reported, yet the spectrum severity and expression patterns angiotensin- converting enzyme 2 (ACE2) in at different developmental stages are largely unknow. Methods: We analysed clinical features a cohort 173 COVID-19 (0-15 yrs.-old) between January 22, 2020 March 25, 2020. systematically examined distribution ACE2 by using combination children's lung biopsies, pluripotent stem cell-derived...
Background: An increasing number of infected children are being encountered with the rapid spread COVID-19, yet vulnerability COVID-19 infection and underlying risk factors largely unknown. It is thought that disease severity might be associated patterns ACE2 expression patient's immune responses.Methods: We retrospectively analysed clinical features in a cohort 234 4 hospitals from Hubei Province (Wuhan Shiyan) Guangdong (Guangzhou Shenzhen), China, between January 22, 2020 March 25, 2020....