A5080267591- Mesenchymal stem cell research
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Gastric Cancer Management and Outcomes
- Immune cells in cancer
- Genomics, phytochemicals, and oxidative stress
- Neurogenesis and neuroplasticity mechanisms
- Colorectal Cancer Screening and Detection
- MicroRNA in disease regulation
- Bone and Joint Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Circular RNAs in diseases
- Liver Disease Diagnosis and Treatment
- RNA modifications and cancer
- S100 Proteins and Annexins
- Colorectal Cancer Surgical Treatments
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- Pancreatic function and diabetes
- Cell Adhesion Molecules Research
- RNA Research and Splicing
Second Hospital of Shandong University
2009-2024
Air Force Medical University
2011-2024
Ministry of Education of the People's Republic of China
2024
Xijing Hospital
2023
University Gastroenterology
2017-2023
Zhejiang University
2020
Weatherford College
2020
Sun Yat-sen University
2006-2016
University of Pennsylvania
2015
Shandong University
2011
Macrophage recruitment and pro-inflammatory differentiation are hallmarks of various diseases, including infection sepsis. Although studies suggest that mitochondria may regulate macrophage immune responses, it remains unclear whether mitochondrial mass affects differentiation. Here, we found lipopolysaccharide (LPS)-activated macrophages possess higher than resting cells. Therefore, this study aimed to explore the functional role molecular mechanisms increased in differentiated macrophages....
We aimed at the effects of long non-coding RNA (lncRNA) SNHG5 on proliferation, metastasis and migration colorectal cancer (CRC) cells. also investigated regulatory relationships among miR-132-3p, CREB5 their roles in CRC. 25 pairs samples containing CRC tissues matched para-tumor were obtained to examine SNHG5, miR-132-3p expression by qRT-PCR or Western blot. The targeted relationship between was confirmed dual luciferase report assay as well pull down assay. cells regulated cell...
Polychlorinated biphenyls (PCB) is a major type of persistent organic pollutants (POPs) that act as endocrine-disrupting chemicals. In the current study, we examined mechanism underlying effect PCB-153 on glucose and lipid metabolism in vivo vitro. We found induced per se worsened high fat diet (HFD)-resulted increase blood level insulin intolerance. addition, HFD-resulted triglyceride content adipose mass. Moreover, concentration-dependently inhibited insulin-dependent uptake accumulation...
ABSTRACT Diabetes mellitus is an established risk factor associated with bisphosphonate-related osteonecrosis of the jaw (BRONJ). Sustained activation Nod-like receptor (NLR) family, pyrin domain-containing protein 3 (NLRP3) inflammasome contributes to persistent inflammation and impaired cutaneous wound healing in diabetic mice human. We have recently demonstrated a compelling linkage between M1 macrophages BRONJ conditions both murine human diseases. The aim this study was determine...
BRAF is among the most frequently mutated oncogenes in human cancers. Multiple small molecule kinase inhibitors have been approved for treating melanoma carrying BRAF-V600 mutations. However, benefits of are generally short-lived. Small molecule-mediated targeted protein degradation has recently emerged as a novel pharmaceutical strategy to remove disease proteins through hijacking cellular ubiquitin proteasome system (UPS). In this study, we developed thalidomide-based heterobifunctional...
This study aims to investigate the effect of miR-21-5p on process colon adenocarcinoma (COAD) cells and its connection with neural cell adhesion molecule L1 (CHL1).Different expressions mRNAs miRNAs were calculated microarray analysis. QRT-PCR western blot performed quantify CHL1 expression. Flow Cytometry, MTT assay, colony formation transwell assay ELISA evaluate propagation invasiveness COAD cells. Dual luciferase reporter was employed scrutinize relationship between miR-21-5P CHL1. We in...
Alzheimer's disease (AD) is the most common cause of dementia. Mitophagy fulfills crucial functions in neurodegenerative disorders and neuronal survival but relationship between mitophagy AD unclear. correlation scores samples control were calculated using single-sample GSEA (ssGSEA) based on two datasets from gene expression omnibus (GEO) database. Mitophagy-related genes (MRGs) differentially expressed (DEGs) screened by WGCNA "limma" package intersected to take genes. These overlapping...
In this study, we explored the competence of adipose-derived stem cells to differentiate into Schwann in vitro. Rat were sequentially treated with various factors beta-mercaptoethanol, all-trans-retinoic acid, followed by a mixture forskolin, basic fibroblast growth factor, platelet-derived factor and heregulin. We found that differentiated displayed morphology cells. Western blotting dual immunofluorescence staining confirmed they produced proteins characteristic for cells, including S100...
MSCs (mesenchymal stem cells) with their versatile growth and differentiation potential are ideal candidates for use in regenerative medicine currently making way into clinical trials, which requires the development of xeno-free protocols culture. In this study, were cultured 10% FCS or 7.5% HPL (human platelet lysate)-supplemented media. We found that both groups showed a comparable morphology, phenotype proliferation. The percentage cells S- G2-/M-phases, however, was slightly up-regulated...
We report compounds 5 (CG416) and 6 (CG428) as two first-in-class tropomyosin receptor kinase (TRK) degraders that target the intracellular domain of TRK. Degraders reduced levels 3 (TPM3)-TRKA fusion protein in KM12 colorectal carcinoma cells inhibited downstream PLCγ1 signaling at sub-nanomolar concentrations. Both also degraded human wild-type TRKA with similar potency. Interestingly, both degraders, especially 6, showed selectivity for degradation endogenous TPM3-TRKA over ectopically...
2,3,5,4′‐Tetrahydroxystilbene‐2‐O‐ β ‐D‐glucoside (TSG), an important monomer extracted from Polygonum multiflorum, can prevent a number of inflammation associated chronic diseases. However, the mechanism involved in TSG inducing anti‐inflammatory role remains unclear. As inducible antioxidant enzyme, Heme oxygenase‐1 (HO‐1), is crucial for protecting mammalian cells against adverse stimuli. Here, we found that treatment strongly induces expression HO‐1 NRF2‐depended manner. Meanwhile,...
Background Nestin is associated with neoplastic transformation, but the mechanisms by which nestin contributes to invasion and malignancy of lung cancer remain unknown. Considering that proliferation necessary for malignant behavior, we investigated mechanism action in association proliferative properties non-small cell (NSCLC). Methods expression was examined NSCLC specimens lines. Associations clinicopathological features, including prognosis markers, were evaluated. Effects knockdown on...
This study was conducted to investigate the effects of ADP dependent glucokinase antisense RNA 1 (ADPGK-AS1)/ miR-205-5p/ zinc finger E-box binding homeobox (ZEB1) on PC cells.Differentially expressed lncRNAs and miRNAs in pancreatic cancer (PC) were identified by microarray analysis. In silico ceRNA analysis find out interactions among lncRNAs, mRNAs. Quantitative real-time PCR (qRT-PCR) utilized examine expression miR-205-5p lncRNA ADPGK-AS1 non-cancerous cells. The association between as...