A5080770831- Epigenetics and DNA Methylation
- Catalytic C–H Functionalization Methods
- Prostate Cancer Treatment and Research
- Cancer-related molecular mechanisms research
- Catalytic Cross-Coupling Reactions
- Cancer-related gene regulation
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- SARS-CoV-2 and COVID-19 Research
- Sulfur-Based Synthesis Techniques
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- MicroRNA in disease regulation
- Radical Photochemical Reactions
- Asymmetric Synthesis and Catalysis
- Bladder and Urothelial Cancer Treatments
- Chemokine receptors and signaling
- Synthesis and Catalytic Reactions
- Viral-associated cancers and disorders
- CAR-T cell therapy research
- RNA Research and Splicing
- Asymmetric Hydrogenation and Catalysis
- Autophagy in Disease and Therapy
- Fluorine in Organic Chemistry
- Immune cells in cancer
Shenzhen Third People’s Hospital
2018-2025
Shenzhen University
2018-2025
Chongqing University of Posts and Telecommunications
2025
Chinese PLA General Hospital
2025
Hebei Medical University
2025
Hebei General Hospital
2025
Sun Yat-sen University
2010-2024
Sun Yat-sen University Cancer Center
2010-2024
Yangzhou University
2016-2024
Zhongnan Hospital of Wuhan University
2017-2024
Summary The pandemic coronavirus SARS‐CoV‐2 in the world has caused a large infected population suffering from COVID‐19. To curb spreading of virus, WHO urgently demanded an extension screening and testing; thus, rapid simple diagnostic method is needed. We applied reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) to achieve detection 30 min. designed four sets LAMP primers (6 each set), targeting viral RNA regions orf1ab, S gene N gene. A colorimetric change was used...
This research examined the relations of social media addiction to college students' mental health and academic performance, investigated role self-esteem as a mediator for relations, further tested effectiveness an intervention in reducing its potential adverse outcomes. In Study 1, we used survey method with sample students (N = 232) found that was negatively associated performance relation between mediated by self-esteem. 2, developed two-stage self-help program. We recruited 38) who met...
It has been recently reported that a side population of cells in nasopharyngeal carcinoma (NPC) displayed characteristics stem-like cancer cells. However, the molecular mechanisms underlying modulation such cell populations NPC remain unclear. Epstein-Barr virus was first identified human tumor to be associated with various malignancies, most notably NPC. LMP2A, encoded latent protein, play roles oncogenic processes. We report by immunostaining our current study LMP2A is overexpressed 57.6%...
BackgroundElevated levels of antibodies against antigens in the Epstein–Barr virus (EBV) lytic phase are important predictive markers for nasopharyngeal carcinoma (NPC) risk. Several lifestyle factors, including smoking, have also been associated with NPC We hypothesized that some specific factors induce transformation EBV from latent to stage and contribute occurrence.
Abstract Epstein–Barr virus (EBV) is implicated as an aetiological factor in B lymphomas and nasopharyngeal carcinoma. The mechanisms of cell-free EBV infection epithelial cells remain elusive. glycoprotein (gB) the critical fusion protein for both cells, determines susceptibility non-B cells. Here we show that neuropilin 1 (NRP1) directly interacts with gB 23–431 . Either knockdown NRP1 or pretreatment soluble suppresses infection. Upregulation by overexpression EGF treatment enhances...
The recent outbreak of betacoronavirus Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which is responsible for the Disease 2019 (COVID-19) global pandemic, has created great challenges in viral diagnosis. existing methods nucleic acid detection are high sensitivity and specificity, but need complex sample manipulation expensive machinery slow down disease detection. Thus, there an urgent demand to develop a rapid, inexpensive, sensitive diagnostic test aid point-of-care...
Abstract Nonsteroidal anti-inflammatory drug derivatives (NSAIDs) are an important class of medications. Here we show a visible-light-promoted photoredox/nickel catalyzed approach to construct enantioenriched NSAIDs via three-component alkyl arylation acrylates. This reductive cross-electrophile coupling avoids preformed organometallic reagents and replaces stoichiometric metal reductants by organic reductant (Hantzsch ester). A broad range functional groups well-tolerated under mild...
Diabetic nephropathy (DN) is one of the most serious complications diabetes. Rhein has been reported to be effective in treating DN. This study aimed investigate role and mechanism rhein treatment DN.High glucose-induced (HG) podocyte injury model streptozocin-induced (STZ) DN mouse were constructed intervened with rhein. Cell viability was detected by Counting Kit-8 (CCK-8) assay. The reactive oxygen species (ROS) level measured flow cytometry. expression Ras-related C3 botulinum toxin...
Abstract Genetically modified HSV-1 viruses serve as promising vectors for tumour therapy and vaccine development. The CRISPR/Cas9 system is one of the most powerful tools precise gene editing genomes organisms. However, whether can precisely efficiently make replacements in genome remains essentially unknown. Here, we reported CRISPR/Cas9-mediated human cells, including knockout replacement large genes. In established cells stably expressing CRISPR/Cas9, gRNA coordination with Cas9 could...
Significance EBV causes nasopharyngeal carcinoma (NPC), an endemic disease in southern China. Unlike infection of B lymphocytes, the molecular mechanisms and outcomes epithelial cells are poorly understood. In vitro, infects lymphoma Mo-MLV insertion region 1 homolog (BMI1)-immortalized (NPECs) poorly. We have now established a protocol to infect sphere-like derived from monolayer NPECs with EBV. Using this model system, we identified nonmuscle myosin heavy chain IIA (NMHC-IIA) as important...
Abstract The application potential of primary amine catalysts in the context Mannich reactions aldehydes with ketimines is exemplified by isatin‐derived and cyclic trifluoromethyl ketimines. Primary exhibit either unusual stereoselectivity or reactivity, which not observable secondary catalysts. Moreover, reversal diastereofacial selectivity between disclosed. These new provide useful methods for syntheses chiral 3‐substituted 3‐amino‐2‐oxindoles dihydroquinazolinones bearing a...
Arterial calcification is a major complication of cardiovascular disease. Oestrogen replacement therapy in postmenopausal women associated with lower levels coronary artery calcification, but its mechanism action remains unclear. Here, we show that oestrogen inhibits the osteoblastic differentiation vascular smooth muscle cells (VSMCs) vitro and arterial vivo by promoting autophagy. Through electron microscopy, GFP-LC3 redistribution, immunofluorescence analyses as well measurement...
Abstract The first arylation strategy for the synthesis of enantioenriched propargylamines is disclosed. This approach, which complementary to previous alkynylation and alkylation strategies, involves a C(sp 2 )−C(sp 3 ) bond formation, based on asymmetric Friedel–Crafts‐type reaction C‐alkynyl imines. Asymmetric Friedel–Crafts reactions with electron‐deficient phenols, longstanding unsolved challenge, have thus been realized time, enabled by combination our recently introduced...
Oncolytic virotherapy can lead to systemic antitumor immunity, but the therapeutic potential of oncolytic viruses in humans is limited due their insufficient ability overcome immunosuppressive tumor microenvironment (TME). Here, we showed that locoregional upregulated expression PD-L1 TME, which was mediated by virus-induced type I and II IFNs. To explore PD-1/PD-L1 signaling as a direct target tissue, developed novel immunotherapeutic herpes simplex virus (HSV), OVH-aMPD-1, expressed...
Abstract SARS-CoV-2 variants have evolved a variety of critical mutations, leading to antigenicity changes and immune escape. The recent emerging Omicron variant attracted global attention due its significant resistance current antibody therapies vaccines. Here, we profiled the mutations other various circulating in parallel by computational interface analysis vitro experimental assays. We identified that lead diminished neutralization efficiency panel 14 antibodies diverse molecular...
A P(NMe2)3-catalyzed [4 + 2] annulation of 2-amino-β-nitrostyrenes and β′-acetoxy allenoates followed by a Zn/AcOH-mediated reduction–hydroamination–isomerization has been developed.
Abstract Purpose: Prostate cancer is the second leading cause of deaths among men in Western counties, which has also occurred Chinese male with markedly increasing incidence recent years. Although mechanism underlying its progression still remains unclear, epigenetic modifications are important ethological parameters. The purpose this study to determine methylation status and function hypermethylatioted 1 (HIC1) prostate progression. Experimental Design: HIC1 promoter was assayed cell...
Arterial calcification is a common cardiovascular disease that initiates from process of osteoblastic differentiation vascular smooth muscle cells (VSMCs). Accumulating evidence has demonstrated microRNAs play an important role in regulating arterial calcification. miR-204 was significantly downregulated calcified human renal arteries patients with uremia; mice, due to 5/6 nephrectomy high-phosphate diet (5/6 NTP); and VSMCs induced by high phosphate concentration. The overexpression...
Background/Aims: Diffuse large B-cell lymphoma (DLBCL), the most common subtype of non-Hodgkin lymphoma, has significant prognostic heterogeneity. This study aimed to generate a prediction model based on autophagy-related genes for DLBCL patients. Methods: Utilizing bioinformatics techniques, we analyzed clinical information and transcriptome data patients from Gene Expression Omnibus (GEO) database. Through unsupervised clustering, identified new molecular subtypes pinpointed differentially...
MicroRNAs (miRNAs) are closely related to cardiovascular diseases, including chronic heart failure (CHF). Endothelial dysfunction can lead failure. The purpose of this study was evaluate the clinical significance miR-1285-3p in CHF patients, with aim identifying a novel and effective biomarker for CHF. At same time, we investigated effect on vascular endothelial cells. Total RNA extracted from plasma samples 106 patients healthy individuals. Quantitative Real-time PCR (qRT-PCR) used detect...
Working memory, a fundamental cognitive function of the brain, necessitates evaluation load intensity due to limited resources. Optimizing can enhance task performance efficiency by preventing resource waste and overload. Therefore, identifying working memory is an essential area research. Deep learning models have demonstrated remarkable potential in load. However, existing neural networks based on electroencephalogram (EEG) decoding primarily focus temporal spatial characteristics while...