A5050461972- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Nanopore and Nanochannel Transport Studies
- Cardiovascular Disease and Adiposity
- Phagocytosis and Immune Regulation
- Atherosclerosis and Cardiovascular Diseases
- Infectious Aortic and Vascular Conditions
- Circular RNAs in diseases
- Genomics and Chromatin Dynamics
- Mechanisms of cancer metastasis
- Advanced biosensing and bioanalysis techniques
- Photosynthetic Processes and Mechanisms
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Systemic Lupus Erythematosus Research
- Photoreceptor and optogenetics research
- Cell Adhesion Molecules Research
- Lipoproteins and Cardiovascular Health
- Chromatin Remodeling and Cancer
- Liver Disease Diagnosis and Treatment
- Inflammasome and immune disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Redox biology and oxidative stress
Semmelweis University
2013-2024
AstraZeneca (United Kingdom)
2018-2022
AstraZeneca (Brazil)
2019
Institut d'Investigació Biomédica de Bellvitge
2016
University of Florida
2014
Interface (United States)
2014
Abstract Circulating extracellular vesicles have emerged as potential new biomarkers in a wide variety of diseases. Despite the increasing interest, their isolation and purification from body fluids remains challenging. Here we studied human pre-prandial 4 hours postprandial platelet-free blood plasma samples well platelet concentrates. Using flow cytometry, found that majority circulating particles within size range lacked common vesicular markers. We identified most these lipoproteins...
This work shows for the first time that exosomes are more resistant to detergents than microvesicles and apoptotic bodies.
Background Understanding the pathogenic role of extracellular vesicles (EVs) in disease and their potential diagnostic therapeutic utility is extremely reliant on in‐depth quantification, measurement identification EV sub‐populations. Quantification EVs has presented several challenges, predominantly due to small size such as exosomes availability various technologies measure nanosized particles, each technology having its own limitations. Materials Methods A standardized methodology...
Extracellular vesicles (EVs) mediate cellular communication through the transfer of active biomolecules, raising interest in using them as biological delivery vehicles for therapeutic drugs. For drug applications, it is important to understand intrinsic safety and toxicity liabilities EVs. Nanoparticles, including EVs, typically demonstrate significant accumulation liver after systemic administration vivo. We confirmed uptake EVs derived from Expi293F cells into HepG2 did not detect any...
Abstract Extracellular Vesicles (EVs) have been intensively explored for therapeutic delivery of proteins. However, methods to quantify cargo proteins loaded into engineered EVs are lacking. Here, we describe a workflow EV analysis at the single‐vesicle and single‐molecule level accurately efficiency different EV‐sorting in promoting loading EVs. Expi293F cells were express fused green fluorescent protein (GFP). High levels GFP secreted was confirmed by Western blotting specific domains, but...
Abstract Extracellular vesicles (EVs) have shown promise as biological delivery vehicles, but therapeutic applications require efficient cargo loading. Here, we developed new methods for CRISPR/Cas9 loading into EVs through reversible heterodimerization of Cas9‐fusions with EV sorting partners. Cas9‐loaded were collected from engineered Expi293F cells using standard methodology, characterized nanoparticle tracking analysis, western blotting, and transmission electron microscopy analysed...
In recent years the study of extracellular vesicles has gathered much scientific and clinical interest. As field is expanding, it becoming clear that better methods for characterization quantification as well standards to compare studies are warranted. The goal present work was find improved parameters characterize vesicle preparations. Here we introduce a simple 96 plate-based total lipid assay determination content protein ratios preparations from various myeloid lymphoid cell lines blood...
Abstract Extracellular vesicles (EVs) have important roles in physiology, pathology, and more recently been identified as efficient carriers of therapeutic cargoes. For study EVs, a single-step, rapid scalable isolation strategy is necessary. Chromatography techniques are widely used for biological material clinical applications EVs net negative charge, anion exchange chromatography (AIEX) strong candidate column based EV isolation. We isolated by AIEX compared them to ultracentrifugation...
Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields biomedicine. Here we investigated the impact sustained exposure cells to fluoroquinolone antibiotic ciprofloxacin on released vesicles. Ciprofloxacin is widely used humans against bacterial infections as well cell cultures Mycoplasma contamination. However, an inducer oxidative stress mitochondrial dysfunction...
ABSTRACT The field of extracellular vesicles (EVs) is an exponentially growing segment biomedical sciences. However, the problems normalisation and quantification EV samples have not been completely solved. Currently, are standardised on basis their protein content sometimes combined with determination particle number. even this approach may result in inaccuracy overestimation concentration. Lipid bilayers indispensable components EVs. Therefore, a lipid‐based quantification, combination...
Abstract Extracellular vesicles (EVs) large‐scale production is a crucial point for the translation of EVs from discovery to application EV‐based products. In October 2021, International Society Vesicles (ISEV), along with support by FET‐OPEN projects, “The Vesicle Foundry” (evFOUNDRY) and “Extracellular natural source tailor‐made nanomaterials” (VES4US), organized workshop entitled “massivEVs” discuss potential challenges This report gives an overview topics discussed during “massivEVs”,...
Aim: Extracellular vesicles (EVs) are desirable delivery vehicles for therapeutic cargoes. We aimed to load EVs with Cre recombinase protein and determine whether functional cells could be improved by using endosomal escape enhancing compounds. Materials & methods: Overexpressed CreFRB was actively loaded into rapalog-induced dimerization CD81FKBP, or passively overexpression in the absence of rapalog. Functional analysed a HEK293 reporter cell line presence Results: The both active passive...
High fat diet (HFD) increases the likelihood of dyslipidemia, which can be a serious risk factor for atherosclerosis, diabetes or hepatosteatosis. Although changes in different blood lipid levels were broadly investigated, such alterations liver tissue have not been studied before. The aim current study was to investigate effect HFD on hepatic triglyceride (TG), diglyceride (DG) and ceramide (CER) expression four key genes involved homeostasis (Pcsk9, Ldlr, Cd36 Anxa2) liver. In addition,...
The prognosis of patients with pancreatic cancer has remained virtually unchanged a high mortality rate compared to other types cancers. An earlier detection would provide time window opportunity for treatment and prevention deaths. In the present study we investigated extracellular vesicle (EV)-associated potential biomarkers by directly assessing EV size-based subpopulations in juice samples chronic pancreatitis or cancer. addition, also studied blood plasma cell line-derived...
The association and co-isolation of low-density lipoproteins (LDL) extracellular vesicles (EVs) have been shown in blood plasma. Here we explore this relationship to better understand the role EVs atherogenesis.
SWI/SNF ATP-dependent chromatin-remodeling complexes have been related to several cellular processes such as transcription, regulation of chromosomal stability, and DNA repair. The Caenorhabditis elegans gene ham-3 (also known swsn-2.1) its paralog swsn-2.2 encode accessory subunits complexes. Using RNA interference (RNAi) assays diverse alleles we investigated whether different functions during C. development since they proteins that are probably mutually exclusive in a given complex. We...
During the Boston Marathon on 15 April 2013, two bombs exploded killing 3 people and injuring 264 others. The just one a half days before scheduled beginning of ISEV 2013 Meeting. leading news in world press was all about explosions when over 700 scientists extracellular vesicle field from around chose to go meeting. meeting held as planned – quiet triumph science terrorism. (Published: 4 December 2013) Citation: Journal Extracellular Vesicles 2 : 23070 - http://dx.doi.org/10.3402/jev.v2i0.23070