A5051626236- Ion Channels and Receptors
- Renal Diseases and Glomerulopathies
- Pain Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Extracellular vesicles in disease
- Sports Performance and Training
- Sports injuries and prevention
- Cardiac electrophysiology and arrhythmias
- Complement system in diseases
- MicroRNA in disease regulation
- Rheumatoid Arthritis Research and Therapies
- Platelet Disorders and Treatments
- Nitric Oxide and Endothelin Effects
- Head and Neck Cancer Studies
- Neurobiology and Insect Physiology Research
- Genetics and Physical Performance
- Cardiac Arrhythmias and Treatments
- Sport Psychology and Performance
- Iron Metabolism and Disorders
- Reproductive System and Pregnancy
- Systemic Lupus Erythematosus Research
- Ion Transport and Channel Regulation
- RNA Interference and Gene Delivery
- Surgical Sutures and Adhesives
University of Debrecen
2016-2025
Semmelweis University
2011-2024
Hungarian School Sport Federation
1998-2024
Eötvös Loránd University
2019-2024
Pharma Patent (Hungary)
2024
Department of Medical Sciences
2024
Széchenyi István University
2024
Spitalul Clinic Judetean de Urgenta Târgu Mureş
2023
Universitatea de Medicină, Farmacie, Științe și Tehnologie „George Emil Palade” din Târgu Mureș
2023
Hungarian University of Sports Science
2017-2022
In recent years, there has been an exponential increase in the number of studies aiming to understand biology exosomes, as well other extracellular vesicles. However, classification membrane vesicles and appropriate protocols for their isolation are still under intense discussion investigation. When isolating vesicles, it is crucial use systems that able separate them, avoid cross-contamination.EVS RELEASED FROM THREE DIFFERENT KINDS OF CELL LINES: HMC-1, TF-1 BV-2 were isolated using two...
Abstract Summary Advances in single-cell technologies have enabled the investigation of T-cell phenotypes and repertoires at unprecedented resolution scale. Bioinformatic methods for efficient analysis these large-scale datasets are instrumental advancing our understanding adaptive immune responses. However, while well-established solutions accessible processing transcriptomes, no streamlined pipelines available comprehensive characterization receptors. Here, we propose Python (Scirpy), a...
During apoptosis, the BCL-2-family protein tBID promotes mitochondrial permeabilization by activating BAX and BAK blocking anti-apoptotic BCL-2 members. Here, we report that can also mediate itself, resulting in release of cytochrome c DNA, caspase activation apoptosis even absence BAK. This previously unrecognized activity depends on helix 6, homologous to pore-forming regions BAK, be blocked pro-survival proteins. Importantly, tBID-mediated independent is physiologically relevant for SMAC...
Vanilloid receptor subtype 1 (VR1) is a ligand-gated channel that can be activated by capsaicin and other vanilloids as well protons heat. In the present study, we have analyzed oligomeric state of VR1. Co-immunoprecipitation differently tagged VR1 molecules indicated form oligomers. Using two different heterologous expression systems endogenous expressed in dorsal root ganglion cells, oligomer formation using perfluoro-octanoic acid polyacrylamide gel electrophoresis. Results were confirmed...
The real time dynamics of vanilloid-induced cytotoxicity and the specific deletion nociceptive neurons expressing wild-type vanilloid receptor (VR1) were investigated. VR1 was C-terminally tagged with either 27-kDa enhanced green fluorescent protein (eGFP) or a 12-amino acid ε-epitope. Upon exposure to resiniferatoxin, VR1eGFP- VR1ε-expressing cells exhibited pharmacological responses similar those untagged VR1. Within seconds exposure, intracellular free calcium ([Ca2+]i) elevated in A...
In recent years the study of extracellular vesicles has gathered much scientific and clinical interest. As field is expanding, it becoming clear that better methods for characterization quantification as well standards to compare studies are warranted. The goal present work was find improved parameters characterize vesicle preparations. Here we introduce a simple 96 plate-based total lipid assay determination content protein ratios preparations from various myeloid lymphoid cell lines blood...
Introduction Microvesicles (MVs), earlier referred to as microparticles, represent a major type of extracellular vesicles currently considered novel biomarkers in various clinical settings such autoimmune disorders. However, the analysis MVs body fluids has not been fully standardized yet, and there are numerous pitfalls that hinder correct assessment these structures. Methods In this study, we analyzed synovial fluid (SF) samples patients with osteoarthritis (OA), rheumatoid arthritis (RA)...
Many microRNAs (miRNAs) are generated from primary transcripts containing multiple clustered stem-loop structures that thought to be recognized and cleaved by the Microprocessor complex as independent units. Here, we uncover an unexpected mode of processing bicistronic miR-15a-16-1 cluster. We find miR-15a is not processed on its own but presence neighboring miR-16-1 same transcript can compensate for this deficiency in cis. Using a CRISPR/Cas9 screen, identify SAFB2 (scaffold attachment...
The recently cloned vanilloid receptor subtype 1 (VR1) is a ligand-gated channel that activated by capsaicin, protons, and heat. We have attempted to develop dominant negative isoform targeting several mutations of VR1 at highly conserved amino acids or residues potential functional importance expressing the mutants in Chinese hamster ovary cells. Mutation three acid putative sixth transmembrane domain disrupts activation both capsaicin resiniferatoxin. binding site this mutant intact,...
The COVID-19 epidemic first appeared at the end of 2019, and it became a worldwide pandemic in quarter 2020. new coronavirus made clear that infectious diseases are able to significantly influence morbidity mortality data countries 21st century, too. There many complex profound socio-economic consequences restrictions relation pandemic. This also resulted an economic crisis with multiple effects on economy society. It is said spatiality has determinative role geographical spreading regional...
Abstract Background Hemolytic uremic syndrome (HUS), characterized by the triad of microangiopathic hemolytic anemia, thrombocytopenia and acute kidney injury (AKI), remains a leading cause pediatric AKI. The complement system has crucial role in pathogenesis atypical (aHUS) eculizumab (ECZ) was approved as standard care for its treatment. two widely forms infection-associated HUS are Shiga toxin-producing E. coli (STEC)-HUS Streptococcus pneumoniae-associated (SP)-HUS. Extrarenal...
The research delves into the nexus between cryptocurrencies and touristic activity, with a special focus on facet of legal regulations. study's objective is to fathom how influence tourism sector, standar ds bear transactions involving within industry, what extent they render adoption in feasible. principal aim unravel interconnection employment tourist services its concurrent governance four handpicked countries: Spain, France, Croatia, Netherlands investigation encompasses, one hand,...
Background Despite well-documented clinical differences across breast-cancer (BC) molecular subtypes and relevant changes in therapeutic interventions over the past decades, there remains a significant lack of up-to-date epidemiologic data real-world outcomes, particularly Central Eastern Europe. Methods This was nationwide, retrospective study using claims databases Hungarian National Health Insurance Fund (NHIF) that included patients who were newly diagnosed with BC between 2011 2020....
The vanilloid receptor VR1 has attracted great interest as a sensory transducer for capsaicin, protons, and heat, therapeutic target. Here we characterize two novel antagonists, KJM429 [<i>N</i>-(4-<i>tert</i>-butylbenzyl)-<i>N</i>′-[4-(methylsulfonylamino)benzyl]thiourea] JYL1421 [<i>N</i>-(4-<i>tert</i>-butylbenzyl)-<i>N</i>′-[3-fluoro-4-(methylsulfonylamino)benzyl]thiourea], with enhanced activity compared capsazepine on rat expressed in Chinese hamster ovary (CHO) cells. JYL1421, the...
The purpose of this study was to investigate the impact fast- and slow-tempo music on 500-m rowing sprint performances. Twenty-two rowers performed sprints 3 times: without music, slow fast tempo music. Strokes per minute (SPM), time completion, (TTC), rated perceived exertion (RPE) were recorded. Although RPE did not differ between conditions, TTC shortest in condition. Further, shorter observed condition contrast control condition, indicating that also enhanced performance. strongest...
Tramadol is an effective analgesic substance widely used in medical practice. Its therapeutic action have been mainly attributed to the activation of mu-opioid receptors as well inhibition neurotransmitter reuptake mechanisms and various voltage- ligand-gated ion channels nociceptive system. As transient receptor potential vanilloid-1 (TRPV1, "the capsaicin receptor") has shown function a central integrator molecule pain sensation, our aim current study was define involvement TRPV1 complex...