A5009351305- Advanced Proteomics Techniques and Applications
- Extracellular vesicles in disease
- Glycosylation and Glycoproteins Research
- Mass Spectrometry Techniques and Applications
- Proteoglycans and glycosaminoglycans research
- Molecular Biology Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- MicroRNA in disease regulation
- Galectins and Cancer Biology
- Studies on Chitinases and Chitosanases
- Fibroblast Growth Factor Research
- Ferroptosis and cancer prognosis
- Carbohydrate Chemistry and Synthesis
- Analytical Chemistry and Chromatography
- Pregnancy and preeclampsia studies
- Mitochondrial Function and Pathology
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Orthopedic Infections and Treatments
- Computational Drug Discovery Methods
- Reproductive System and Pregnancy
- Air Quality and Health Impacts
HUN-REN Research Centre for Natural Sciences
2015-2024
Institute of Organic Chemistry
2013-2024
Eötvös Loránd University
2023
Hungarian Research Network
2021-2023
Research Network (United States)
2021-2023
Semmelweis University
2010-2023
Uppsala University
2023
Institute of Materials and Environmental Chemistry
2022
Hungarian Academy of Sciences
2010-2020
Boston University
2014
Abstract Circulating extracellular vesicles have emerged as potential new biomarkers in a wide variety of diseases. Despite the increasing interest, their isolation and purification from body fluids remains challenging. Here we studied human pre-prandial 4 hours postprandial platelet-free blood plasma samples well platelet concentrates. Using flow cytometry, found that majority circulating particles within size range lacked common vesicular markers. We identified most these lipoproteins...
Abstract In this study we tested whether a protein corona is formed around extracellular vesicles (EVs) in blood plasma. We isolated medium‐sized nascent EVs of THP1 cells as well Optiprep‐purified platelets, and incubated them EV‐depleted plasma from healthy subjects patients with rheumatoid arthritis. were subjected to differential centrifugation, size exclusion chromatography, or density gradient ultracentrifugation followed by mass spectrometry. Plasma protein‐coated had higher compared...
Introduction Microvesicles (MVs), earlier referred to as microparticles, represent a major type of extracellular vesicles currently considered novel biomarkers in various clinical settings such autoimmune disorders. However, the analysis MVs body fluids has not been fully standardized yet, and there are numerous pitfalls that hinder correct assessment these structures. Methods In this study, we analyzed synovial fluid (SF) samples patients with osteoarthritis (OA), rheumatoid arthritis (RA)...
Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields biomedicine. Here we investigated the impact sustained exposure cells to fluoroquinolone antibiotic ciprofloxacin on released vesicles. Ciprofloxacin is widely used humans against bacterial infections as well cell cultures Mycoplasma contamination. However, an inducer oxidative stress mitochondrial dysfunction...
Extracellular Vesicles (EVs) play pivotal roles in cell-to-cell and inter-kingdom communication. Despite their relevant biological implications, the existence role of plant EVs released into environment has been unexplored. Herein, we purified round-shaped small vesicles by differential ultracentrifugation a sampling solution containing root exudates hydroponically grown tomato plants. Biophysical analyses, means dynamic light scattering, microfluidic resistive pulse sensing scanning...
In pathological conditions, F0F1-ATPase hydrolyzes ATP in an attempt to maintain mitochondrial membrane potential. Using thermodynamic assumptions and computer modeling, we established that potential can be more negative than the reversal of adenine nucleotide translocase (ANT) but positive F0F1-ATPase. Experiments on isolated mitochondria demonstrated that, when electron transport chain is compromised, reverses, maintained as long matrix substrate-level phosphorylation functional, without a...
Micro- and nano-sized vesicles (MVs NVs, respectively) from edible plant resources are gaining increasing interest as green, sustainable, biocompatible materials for the development of next-generation delivery vectors. The isolation complex matrix is a significant challenge considering trade-off between yield purity. Here, we used differential ultracentrifugation (dUC) bulk production MVs NVs tomato (Solanum lycopersicum L.) fruit analyzed their physical morphological characteristics...
Regulatory T cells (Treg) are mandatory elements in the maintenance of human pregnancy, but their de novo differentiation has not been completely exposed. HSPE1 chaperone expressing trophoblast may have a role it. Trophoblast-derived extracellular vesicles (EVs), either at feto–maternal interface or circulation, target CD4+ cells. We hypothesized that HSPE1-associated trophoblastic cell line (BeWo)-derived EVs active mediators Treg differentiation. proved first recombinant promote vitro....
The cellular vesicle is a fluid-filled structure separated from the surrounding environment by biological membrane. Here, we isolated nanovesicles (NVs) juice of clementines using discontinuous density gradient ultracentrifugation method. To gain information about protein content vesicles, mass spectrometry-based organelle proteomics and bioinformatics were applied to exosome-like fraction in 1 mol/L sucrose/D2O cushion. Analysis 1018 identified proteins revealed highly complex mixture...
The release of extracellular vesicles (EVs) is increased under cellular stress and cardiomyocyte damaging conditions. However, whether the cardiomyocyte-derived EVs eventually reach systemic circulation their number in bloodstream reflects cardiac injury, remains unknown. Wild type C57B/6 conditional transgenic mice expressing green fluorescent protein (GFP) by cardiomyocytes were studied lipopolysaccharide (LPS)-induced inflammatory response syndrome (SIRS). separated both from...
Cardiomyopathies are leading causes of human mortality. Recent data indicate that the cardiomyocyte-derived extracellular vesicles (EVs) released upon cardiac injury present in circulation. This paper aimed to analyze EVs under normal and hypoxic conditions by H9c2 (rat), AC16 (human) HL1 (mouse) cell lines. Small (sEVs), medium (mEVs) large (lEVs) were separated from a conditioned combination gravity filtration, differential centrifugation tangential flow filtration. The characterized...
Abstract Extracellular vesicles (EVs) are critical mediators of intercellular communication and hold promise as biomarkers therapeutic targets in cancer, but their molecular alterations remain poorly understood. Protein glycosylation is a frequent post-translational modification; however, most EV studies focus only on proteomics, while mapping changes proteins still underrepresented. To address this shortcoming, we analyzed the proteomic, N -glycoproteomic, chondroitin/dermatan sulfate...
A glycoprotein may contain several sites of glycosylation, each which is heterogeneous. As a consequence glycoform diversity and signal suppression from nonglycosylated peptides that ionize more efficiently, typical reversed-phase LC-MS bottom-up proteomics database searching workflows do not perform well for identification site-specific glycosylation complex glycoproteins. We present an system enrichment, separation, analysis glycopeptides glycoproteins (>4 N-glycosylation sequons) in...
Extracellular matrixes comprise glycoproteins, glycosaminoglycans and proteoglycans that order the environment through which cells receive signals communicate. Proteomic glycomic molecular signatures from tissue surfaces can add diagnostic power to immunohistochemistry workflows. Acquired in a spatially resolved manner, such proteomic information help characterize disease processes be easily applied clinical setting. Our aim toward obtaining integrated omics datasets was develop first...
The prognosis of patients with pancreatic cancer has remained virtually unchanged a high mortality rate compared to other types cancers. An earlier detection would provide time window opportunity for treatment and prevention deaths. In the present study we investigated extracellular vesicle (EV)-associated potential biomarkers by directly assessing EV size-based subpopulations in juice samples chronic pancreatitis or cancer. addition, also studied blood plasma cell line-derived...
A straightforward approach has been developed to distinguish core and antenna fucosylation in glycopeptides. The method does not require derivatization can be easily adapted into a proteomics workflow. key aspect is use low collision energy collision-induced dissociation (CID) (on quadrupole time-of-flight type instrument) when only single-step fragmentation processes occur. Low should show the precursor ion as largest peak spectrum; survival yield ideally over 50%, this obtained at ca. 30%...
Lung cancer is one of the most commonly diagnosed types. Studying molecular changes that occur in lung important to understand tumor formation and identify new therapeutic targets early markers disease decrease mortality. Glycosaminoglycan chains play roles various signaling events microenvironment. Therefore, we have determined quantity sulfation characteristics chondroitin sulfate heparan formalin-fixed paraffin-embedded human tissue samples belonging different types as well adjacent...
Abstract Lung cancer is a severe disease for which better diagnostic and therapeutic approaches are urgently needed. Increasing evidence implies that aberrant protein glycosylation plays crucial role in the pathogenesis progression of lung cancer. Differences patterns have been previously observed between healthy cancerous samples as well different subtypes, suggests untapped potential. In addition, understanding changes mediated by may shed light on possible novel targets personalized...
Assembly and disassembly of protein-protein complexes needs to be dynamically controlled phosphoswitches based on linear motifs are crucial in this process. Extracellular signal-regulated kinase 2 (ERK2) recognizes a linear-binding motif at the C-terminal tail (CTT) ribosomal S6 1 (RSK1), leading phosphorylation subsequent activation RSK1. The CTT also contains classical PDZ domain-binding which binds RSK substrates (e.g. MAGI-1). We show that autophosphorylation disordered promotes...