A5023826471- Autophagy in Disease and Therapy
- Cellular transport and secretion
- Lysosomal Storage Disorders Research
- Neurobiology and Insect Physiology Research
- Plant Reproductive Biology
- Animal Behavior and Reproduction
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Fatty Acid Research and Health
- Ubiquitin and proteasome pathways
- Wnt/β-catenin signaling in development and cancer
- Toxoplasma gondii Research Studies
- Proteins in Food Systems
- Cannabis and Cannabinoid Research
- Genetics, Aging, and Longevity in Model Organisms
- Silk-based biomaterials and applications
- Calcium signaling and nucleotide metabolism
- Clay minerals and soil interactions
- Plant pathogens and resistance mechanisms
- Arsenic contamination and mitigation
- Iron oxide chemistry and applications
- Nanoparticles: synthesis and applications
- Mosquito-borne diseases and control
- Insect and Arachnid Ecology and Behavior
Eötvös Loránd University
2016-2025
At the onset of metamorphosis, Drosophila salivary gland cells undergo a burst glue granule secretion to attach forming pupa solid surface. Here, we show that excess granules evading exocytosis are degraded via direct fusion with lysosomes, secretory granule-specific autophagic process known as crinophagy. We find tethering complex HOPS (homotypic and protein sorting); small GTPases Rab2, Rab7, its effector, PLEKHM1; SNAP receptor consisting Syntaxin 13, Snap29, Vamp7 all required for...
Micro- and nano-sized vesicles (MVs NVs, respectively) from edible plant resources are gaining increasing interest as green, sustainable, biocompatible materials for the development of next-generation delivery vectors. The isolation complex matrix is a significant challenge considering trade-off between yield purity. Here, we used differential ultracentrifugation (dUC) bulk production MVs NVs tomato (Solanum lycopersicum L.) fruit analyzed their physical morphological characteristics...
Abstract Loss of epithelial cell polarity and tissue disorganization are hallmarks carcinogenesis, in which Ca 2+ signaling plays a significant role. Here we demonstrate that the plasma membrane pump PMCA4 (ATP2B4) is downregulated luminal breast cancer, this associated with shorter relapse-free survival patients A B1 subtype tumors. Using MCF-7 cancer model show silencing results loss while forced increase PMCA4b expression induces polarization promotes lumen formation. We identify Arf6 as...
At the onset of Drosophila metamorphosis, plenty secretory glue granules are released from salivary gland cells and is deposited on ventral side forming (pre)pupa to attach it a dry surface. Prior this, poorly understood maturation process takes place during which gradually grow via homotypic fusions, their contents reorganized. Here we show that small GTPase Rab26 localizes immature (smaller, non-acidic) its presence prevents vesicle acidification. mutation accelerates maturation,...
Two related multisubunit tethering complexes promote endolysosomal trafficking in all eukaryotes: Rab5-binding CORVET that was suggested to transform into Rab7-binding HOPS. We have previously identified miniCORVET, containing Drosophila Vps8 and three shared core proteins, which are required for endosome maturation upstream of HOPS highly endocytic cells (Lőrincz et al., 2016a). Here, we show overexpression inhibits HOPS-dependent routes including late maturation, autophagosome-lysosome...
Abstract We present facile methods to obtain purified sporopollenin exine capsules, and provide mass balances for classical novel purification procedures. An ionic liquid, tetrabutyl phosphonium hydroxide turned out be the most effective in removing intine wall. The capsules were investigated by fluorescent microscopy, AFM, solid-state NMR infrared Raman spectroscopy. latter two showed that sunflower rape exines have different proportions of O-aliphatic aromatic constituents. Purified coated...
Bulk production and release of glue containing secretory granules takes place in the larval salivary gland during Drosophila development order to attach metamorphosing animal a dry surface. These undergo maturation process prepare for exocytosis, which includes homotypic fusions increase size granules, vesicle acidification ion uptake. The steroid hormone 20-hydroxyecdysone is known be required first last steps this process: synthesis secretion, respectively. Here we show that B1 isoform...
Structural plumage colour is one of the most enigmatic sexually selected traits. The information content structural variation debated and heterogeneity findings hard to explain because proximate background within-species differences very scarcely studied. We combined measurements feather macrostructure nanostructure within-population variability in blue tit crown reflectance. found that sexual dichromatism aspects reflectance was explained only by macrostructure, while nanostructural...
One of the most extensively investigated sexually selected phenotypic characters is coloration. A knowledge individual variation in anatomical basis coloration essential for better understanding link between colour expression and its information content. We association great tit (Parus major) black crown feather structure [brightness, ultraviolet (UV) chroma] at level, using transmission electron microscopic, photographic spectrometric techniques. quantified three different levels spatial...
Trans fatty acids (TFAs) are not synthesized in the human body but generally ingested substantial amounts. The widespread view that TFAs, particularly those of industrial origin, unhealthy and contribute to obesity, cardiovascular diseases diabetes is based mostly on vivo studies, underlying molecular mechanisms remain be elucidated. Here, we used a hepatoma model palmitate-induced lipotoxicity compare metabolism effects representative ruminant elaidate vaccenate, respectively, with...
Both constitutive and regulated secretion require cell organelles that are able to store release the secretory cargo. During development, larval salivary gland of Drosophila initially produces high amount glue-containing small immature granules, which then fuse with each other reach their normal 3-3.5 μm in size. Following burst secretion, obsolete glue granules directly late endosomes or lysosomes by a process called crinophagy, leads fast degradation recycling However, hindering...
Endocytosis and autophagy are evolutionarily conserved degradative processes in all eukaryotes. Both pathways converge to the lysosome where cargo is degraded. Improper lysosomal degradation observed many human pathologies, so its regulatory mechanisms important understand. Sec20/BNIP1 (BCL2/adenovirus E1B 19 kDa protein-interacting protein 1) a BH3 (Bcl-2 homology 3) domain-containing SNARE (soluble N-ethylmaleimide-sensitive factor-attachment receptors) that has been suggested promote...
Abstract Loss of epithelial cell polarity and tissue disorganization are hallmarks carcinogenesis, in which Ca 2+ signaling plays a significant role. Here we demonstrate that the plasma membrane pump PMCA4 (ATP2B4) is downregulated luminal breast cancer, this associated with shorter relapse-free survival patients A B1 subtype tumors. Using MCF-7 cancer model show silencing results loss while forced increase PMCA4b expression induces polarization promotes lumen formation 2D 3D cultures. We...