A5077581657- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Fibroblast Growth Factor Research
- Extracellular vesicles in disease
- Polysaccharides Composition and Applications
- Prostate Cancer Treatment and Research
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Advanced Fiber Laser Technologies
Semmelweis University
2022-2024
HUN-REN Research Centre for Natural Sciences
2021-2024
Institute of Organic Chemistry
2024
Hungarian Research Network
2021
Research Network (United States)
2021
Abstract Extracellular vesicles (EVs) are critical mediators of intercellular communication and hold promise as biomarkers therapeutic targets in cancer, but their molecular alterations remain poorly understood. Protein glycosylation is a frequent post-translational modification; however, most EV studies focus only on proteomics, while mapping changes proteins still underrepresented. To address this shortcoming, we analyzed the proteomic, N -glycoproteomic, chondroitin/dermatan sulfate...
Lung cancer is one of the most commonly diagnosed types. Studying molecular changes that occur in lung important to understand tumor formation and identify new therapeutic targets early markers disease decrease mortality. Glycosaminoglycan chains play roles various signaling events microenvironment. Therefore, we have determined quantity sulfation characteristics chondroitin sulfate heparan formalin-fixed paraffin-embedded human tissue samples belonging different types as well adjacent...
Chronic liver diseases have both high incidence and mortality rates; therefore, a deeper understanding of the underlying molecular mechanisms is essential. We determined content sulfation pattern chondroitin sulfate (CS) heparan (HS) in human hepatocellular carcinoma cirrhotic tissues, considering etiology diseases. A variety pathological conditions such as alcoholic disease, hepatitis B C virus infections, primary sclerosing cholangitis were studied. Major differences observed total...
Abstract Heparan sulphates (HSs), a specific class of glycosaminoglycans (GAGs), are important participants cellular signalling. Analytical characterization GAGs requires complex sample preparation workflow. Although detailed stability and recovery study is available for the chondroitin sulphate GAG class, literature concerning HS incomplete in this regard. Therefore, our aim was to systematically investigate various parameters that could potentially influence samples when performing...
Abstract Chondroitin sulfate (CS) is a widely studied class of glycosaminoglycans, responsible for diverse biological functions. Structural analysis CS generally based on disaccharide analysis. Sample preparation key analytical issue in this case. However, detailed study the stability and recovery CS-derived species has been lacking so far. We have found that solvent exchange, general, vacuum evaporation (SpeedVac) much preferable than lyophilization. Moreover, case aqueous solutions, higher...
Prostate cancer is one of the most frequent types among men. Several biomarkers and risk assessment methods are already available; however, enhancing their selectivity sensitivity still necessary. For improving therapeutic decisions, both basic clinical research studies ongoing for a better understanding underlying molecular mechanisms. The enzymatic digests heparan sulfate (HS) chondroitin (CS) chains were investigated in tissue samples taken from patients with prostate (PCa) benign...