A5076845167- Genetics, Aging, and Longevity in Model Organisms
- Endoplasmic Reticulum Stress and Disease
- Redox biology and oxidative stress
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Nanoparticles: synthesis and applications
- Coenzyme Q10 studies and effects
- Autophagy in Disease and Therapy
- Nuclear Structure and Function
- Phagocytosis and Immune Regulation
- Microtubule and mitosis dynamics
- Spaceflight effects on biology
- Biochemical Acid Research Studies
- Glutathione Transferases and Polymorphisms
- Fungal and yeast genetics research
- Neuroinflammation and Neurodegeneration Mechanisms
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Tea Polyphenols and Effects
- Advanced Glycation End Products research
- Nanoplatforms for cancer theranostics
- Sulfur Compounds in Biology
- DNA Repair Mechanisms
- Glycosylation and Glycoproteins Research
- Neurogenesis and neuroplasticity mechanisms
Center for Biomedical Research of La Rioja
2016-2025
University of Córdoba
2021
Consejo Superior de Investigaciones Científicas
2011
Centro de Investigación del Cáncer
2010-2011
Universidad de Salamanca
1993-2011
Technische Universität Braunschweig
2005-2010
Frontotemporal lobar degeneration is a progressive neurodegenerative syndrome that the second most common cause of early-onset dementia. Mutations in progranulin gene are major familial frontotemporal [Baker M, et al. (2006) Nature 442:916–919 and Cruts 442:920–924]. Although involved wound healing, inflammation, tumor growth, its role nervous system mechanism by which insufficient levels result neurodegeneration poorly understood [Eriksen Mackenzie (2008) J Neurochem 104:287–297]. We have...
Quercetin is a major flavonoid in the human diet and most commonly used studies of biological activity. Most knowledge about its effects has originated from vitro while vivo data are scarce. mostly occurs foodstuffs as glycosides that deglycosylated during absorption further submitted to different conjugation reactions. Methylation isorhamnetin (quercetin 3′-O-methylether) or tamarixetin 4′-O-methylether) seems be an important process quercetin metabolism. In this work, 3′- 4′-O-methylated...
Wnt signalling pathways have extremely diverse functions in animals, including induction of cell fates or tumours, guidance movements during gastrulation, and the polarity. can induce polar changes cellular morphology by a remodelling cytoskeleton. However, how activation Frizzled receptor induces cytoskeleton rearrangement is not well understood. We show, an depth 4-D microscopy analysis, that Caenorhabditis elegans pathway signals to CED-10/Rac via two separate branches regulate modulation...
Laminopathies are diseases characterized by defects in nuclear envelope structure. A well-known example is Emery-Dreifuss muscular dystrophy, which caused mutations the human lamin A/C and emerin genes. While most proteins ubiquitously expressed, laminopathies often affect only a subset of tissues. The molecular mechanisms underlying these tissue-specific manifestations remain elusive. We hypothesize that different functional subclasses genes might be differentially affected specific...
Functional in vivo studies on the mitochondrial thioredoxin system are hampered by embryonic or larval lethal phenotypes displayed murine Drosophila knock-out models. Thus, access to alternative metazoan models for is of critical importance.We report here characterization Caenorhabditis elegans that composed genes trx-2 and trxr-2. We demonstrate proteins 2 (TRX-2) reductase (TRXR-2) localize mitochondria several cells tissues nematode trxr-2 upregulated upon induction unfolded protein...
The transcriptomes of model organisms have been defined under specific laboratory growth conditions. standard protocol for Caenorhabditis elegans and maintenance is 20°C on an Escherichia coli diet. Temperatures ranging from 15°C to 25°C or feeding with other species bacteria are considered physiological conditions, but the effect these conditions worm transcriptome has not well characterized. Here, we compare global gene expression profile reference strain (N2) grown at 15°C, 20°C, two...
Gene expression is generally regulated by recruitment of transcription factors and RNA polymerase II (RNAP II) to specific sequences in the gene promoter region. The Integrator complex mediates processing small nuclear RNAs (snRNAs) as well initiation release paused RNAP at genes response growth factors. Here we show that C. elegans, disruption leads located downstream snRNA loci via a non-conventional mechanism based on lack snRNAs. read-through generates long chimeric containing snRNA,...
The gsr-1 gene encodes the only glutathione reductase in Caenorhabditis elegans and loss of function alleles have a fully penetrant embryonic lethal phenotype. Therefore, maintenance redox homeostasis is essential for nematode survival. We report here that impairment nonsense-mediated mRNA decay (NMD) pathway suppresses lethality mutants, allowing their normal development growth. This NMD dependent suppression requires cth-1 cth-2 encode, respectively, two isoforms cystathionine-g-lyase...
Mammalian TFEB and TFE3, as well their ortholog in Caenorhabditis elegans HLH-30, play an important role mediating cellular response to a variety of stress conditions, including nutrient deprivation, oxidative stress, pathogen infection. In this study, we identify novel mechanism TFEB/HLH-30 regulation through cysteine-mediated redox switch. Under TFEB-C212 undergoes oxidation, allowing the formation intermolecular disulfide bonds that result oligomerization. oligomers display increased...
During development, the processes of cell division, differentiation and apoptosis must be precisely coordinated in order to maintain tissue homeostasis. The nematode C. elegans is a powerful model system which study death its control. apoptotic cells condense form refractile corpses under differential interference contrast (DIC) microscopy. Activation GTPase CED-10 (Rac) neighbouring mediates recognition engulfment corpse. After inclusion engulfed corpse phagosome, different proteins are...
Thioredoxins are a class of evolutionarily conserved proteins that have been demonstrated to play key role in many cellular processes involving redox reactions. We report here the genetic and biochemical characterization Caenorhabditis elegans TRX-3, first metazoan thioredoxin with an intestine-specific expression pattern. By using green fluorescent protein reporters we found TRX-3 is expressed both cytoplasm nucleus intestinal cells, prominent localization at apical membrane. Although...
X-linked sideroblastic anemia with ataxia (XLSA/A) is a rare inherited disorder characterized by mild and ataxia. XLSA/A caused mutations in the ABCB7 gene, which encodes member of ATP-binding cassette transporter family. Studies yeast, mammalian cells, mice have shown that functions transport iron-sulfur (Fe-S) clusters into cytoplasm. To further investigate mechanism this disease, we identified Caenorhabditis elegans homologue abtm-1. We studied function abtm-1 using mutants RNAi....
Pterygium is a lesion of the eye surface which involves cell proliferation, migration, angiogenesis, fibrosis, and extracellular matrix remodelling. Surgery only approved method to treat this disorder, but high recurrence rates are common. Recently, it has been shown in mouse model that treatment with doxycycline resulted reduction pterygium lesions. Here we study mechanism(s) action by achieves these results, using massive sequencing techniques. Surgically removed pterygia from 10...
Basic Helix-Loop-Helix (bHLH) transcription factors TFEB/TFE3 and HLH-30 are key regulators of autophagy induction lysosomal biogenesis in mammals C. elegans, respectively. While much is known about the regulation TFEB/TFE3, how subcellular dynamics transactivation modulated yet poorly understood. Thus, elucidating elegans will provide evolutionary insight into mechanisms governing function bHLH factor family. We report here that retained cytoplasm mainly through its conserved Ser201 residue...
Apoptotic cell death is an integral part of turnover in many tissues, and proper corpse clearance vital to maintaining tissue homeostasis all multicellular organisms. Even tissues with high cellular turnover, apoptotic cells are rarely seen because efficient mechanisms healthy individuals. In Caenorhabditis elegans, two parallel partly redundant conserved pathways act engulfment. The pathway for cytoskeletal rearrangement requires the small GTPase CED-10 Rac1 acting surround dead cell. Rac...