A5079142953- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Renal and related cancers
- Cancer-related gene regulation
- Genetics, Aging, and Longevity in Model Organisms
- RNA and protein synthesis mechanisms
- Developmental Biology and Gene Regulation
- RNA Interference and Gene Delivery
- Neurobiology and Insect Physiology Research
- RNA modifications and cancer
- Virus-based gene therapy research
- Cellular transport and secretion
- Reproductive Biology and Fertility
- Advanced biosensing and bioanalysis techniques
- Marine Ecology and Invasive Species
- Plant Molecular Biology Research
- Microtubule and mitosis dynamics
- Marine and coastal plant biology
- DNA and Nucleic Acid Chemistry
- Phagocytosis and Immune Regulation
- DNA Repair Mechanisms
- Pancreatic and Hepatic Oncology Research
University of Göttingen
2020-2024
Tissue Dynamics (Israel)
2024
Max Planck Institute for Multidisciplinary Sciences
2023-2024
Max Planck Institute for Biophysical Chemistry
2010-2020
University of Cambridge
2013-2018
Wellcome/Cancer Research UK Gurdon Institute
2013-2018
Wellcome Trust
2013-2018
Google (United States)
2014
Wellcome/MRC Cambridge Stem Cell Institute
2014
Medical Research Council
2014
Primordial germ cells (PGCs) and preimplantation embryos undergo epigenetic reprogramming, which includes comprehensive DNA demethylation. We found that PRMT5, an arginine methyltransferase, translocates from the cytoplasm to nucleus during this process. Here we show conditional loss of PRMT5 in early PGCs causes complete male female sterility, preceded by upregulation LINE1 IAP transposons as well activation a damage response. Similarly, maternal-zygotic also leads upregulation. is...
The mammalian homotypic fusion and vacuole protein sorting ( HOPS ) complex is comprised of six subunits: VPS11 , VPS16 VPS18 VPS39 VPS41 the Sec1/Munc18 SM family member VPS33A . Human has been predicted to be a tethering required for intracellular compartments with lysosomes, but it remains unclear whether all subunits are required. We showed that whole endosomes lysosomes by monitoring delivery endocytosed fluorescent dextran in cells depleted individual proteins. used crystal structure /...
Early mouse development is accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2), which essential for embryonic development. Here we show that genome-wide accumulation of H3K9me2 crucial postimplantation development, and coincides with redistribution enhancer zeste homolog 2 (EZH2)-dependent 27 trimethylation (H3K27me3). Loss G9a or EZH2 results upregulation distinct gene sets involved cell cycle regulation, germline...
Eukaryotes package DNA into nucleosomes that contain a core of histone proteins. During replication, are disrupted and re-assembled with newly synthesized histones DNA. Despite much progress, it is still unclear why higher eukaryotes multiple genes, how chromatin assembly controlled, these processes coordinated cell cycle progression. We used null mutation Drosophila melanogaster to show supply levels, provided by defined number transgenic regulate the length S phase during cycle. Lack de...
Early mammalian development entails transit through naive pluripotency towards post-implantation epiblast, which subsequently gives rise to primordial germ cells (PGC), the founding germline population. To investigate these cell fate transitions, we developed a compound-reporter track cellular identity in model of PGC specification (PGC-like cells; PGCLC), and coupled it with genome-wide CRISPR screening. We identify key genes both for exit from acquisition fate, characterise central role...
Wnt signalling pathways have extremely diverse functions in animals, including induction of cell fates or tumours, guidance movements during gastrulation, and the polarity. can induce polar changes cellular morphology by a remodelling cytoskeleton. However, how activation Frizzled receptor induces cytoskeleton rearrangement is not well understood. We show, an depth 4-D microscopy analysis, that Caenorhabditis elegans pathway signals to CED-10/Rac via two separate branches regulate modulation...
Epigenetic inheritance during DNA replication requires an orchestrated assembly of nucleosomes from parental and newly synthesized histones. We analyzed Drosophila HisC mutant embryos harboring a deletion all canonical histone genes, in which nucleosome relies on histones cell cycle 14 onward. Lack new synthesis leads to more accessible chromatin reduced occupancy, since only are available. This up-regulated spurious transcription, whereas the control developmental transcriptional program is...
Self-renewing stem cells are pools of undifferentiated cells, which maintained in cellular niche environments by distinct tissue-specific signalling pathways. In Drosophila melanogaster, female germline (GSCs) a somatic the gonads BMP signalling. Here we report novel function kinase Bällchen (BALL), showing that its cell autonomous role is to maintain self-renewing capacity GSCs independent ball mutant eliminated from and subsequently differentiate into mature eggs, indicating BALL largely...
Stem cells continuously generate differentiating daughter and are essential for tissue homeostasis development. Their capacity to self-renew as undifferentiated actively dividing is controlled by either external signals from a cellular environment, the stem cell niche, or asymmetric distribution of fate determinants during division. Here we report that protein kinase Bällchen (BALL) required prevent differentiation well maintain normal proliferation neuronal Drosophila melanogaster, called...
Primordial germ cells (PGCs) are the embryonic precursors of sperm and oocytes, which transmit genetic/epigenetic information across generations. Mouse PGC subsequent gamete development can be fully reconstituted in vitro, opening up new avenues for cell studies biomedical research. However, PGCs show molecular differences between rodents humans. Therefore, to establish an vitro system that is closely related humans, we studied vivo common marmoset monkey Callithrix jacchus ( cj ). Gonadal...
ABSTRACT Early mammalian development entails a series of cell fate transitions that includes transit through naïve pluripotency to post-implantation epiblast. This subsequently gives rise primordial germ cells (PGC), the founding population germline lineage. To investigate gene regulatory networks control these critical decisions, we developed compound-reporter system track cellular identity in model PGC specification (PGC-like cells; PGCLC), and coupled it with unbiased genome-wide CRISPR...