A5073466898- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Renal and related cancers
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Interference and Gene Delivery
- Chromosomal and Genetic Variations
- Sexual Differentiation and Disorders
- Animal Genetics and Reproduction
- Genetics and Neurodevelopmental Disorders
- Reproductive System and Pregnancy
- Neuroscience and Neuropharmacology Research
- Reproductive Biology and Fertility
- RNA Research and Splicing
The Gurdon Institute
2018-2023
University of Cambridge
2018-2023
Wellcome Trust
2018-2023
Institute of Molecular Biology
2012-2019
DKFZ-ZMBH Alliance
2012
Heidelberg University
2012
Gonadal development is a complex process that involves sex determination followed by divergent maturation into either testes or ovaries
Article26 September 2018Open Access Transparent process Metabolic regulation of pluripotency and germ cell fate through α-ketoglutarate Julia Tischler orcid.org/0000-0003-4665-4141 Wellcome Trust/Cancer Research UK Gurdon Institute, University Cambridge, Search for more papers by this author Wolfram H Gruhn John Reid MRC Biostatistics Unit, Cambridge Institute Public Health, Biomedical Campus, The Alan Turing British Library, London, Edward Allgeyer orcid.org/0000-0002-2187-4423 Florian...
Abstract PRDM14 is a crucial regulator of mouse primordial germ cells (mPGCs), epigenetic reprogramming and pluripotency, but its role in the evolutionarily divergent regulatory network human PGCs (hPGCs) remains unclear. Besides, previous knockdown study indicated that might be dispensable for cell fate. Here, we decided to use inducible degrons more rapid comprehensive depletion. We show loss results significantly reduced specification efficiency an aberrant transcriptome hPGC-like...
Epigenetic resetting in the mammalian germ line entails acute DNA demethylation, which lays foundation for gametogenesis, totipotency, and embryonic development. We characterize epigenome of hypomethylated human primordial cells (hPGCs) to reveal mechanisms preventing widespread derepression genes transposable elements (TEs). Along with loss methylation, we show that hPGCs exhibit a profound reduction repressive histone modifications resulting diminished heterochromatic signatures at most...
Human primordial germ cells (hPGCs), the precursors of sperm and eggs, are specified during weeks 2−3 after fertilization. Few studies on ex vivo in vitro cultured human embryos reported plausible hPGCs embryonic day (E) 12−13 an E16−17 gastrulating embryo. In vitro, hPGC-like (hPGCLCs) can be from intermediary pluripotent stage or peri-gastrulation precursors. Here, we explore broad spectrum hPGCLC how different impact development. We show that resetting give rise to hPGCLCs (rhPGCLCs)...
The precise control of gene expression by transcription factor networks is crucial to organismal development. predominant approach for mapping factor-chromatin interactions has been chromatin immunoprecipitation (ChIP). However, ChIP requires a large number homogeneous cells and antisera with high specificity. A second approach, DamID, the drawback that levels Dam methylase are toxic. Here, we modify our targeted DamID (TaDa) enable cell type-specific in mammalian systems, generating an...
Learning is essential for survival and controlled by complex molecular mechanisms including regulation of newly synthesized mRNAs that are required to modify synaptic functions. Despite the well-known role RNA-binding proteins (RBPs) in mRNA functionality, their detailed during memory consolidation poorly understood. This study focuses on brain function RBP Gadd45α (growth arrest DNA damage-inducible protein 45 alpha, encoded Gadd45a gene). Here, we find hippocampal long-term potentiation...
Abstract PRDM14 is a crucial regulator of mouse primordial germ cells (mPGC), epigenetic reprogramming and pluripotency, but its role in the evolutionarily divergent regulatory network human PGCs (hPGCs) remains unclear. Besides, previous knockdown study indicated that might be dispensable for cell fate. Here, we decided to use inducible degrons more rapid comprehensive depletion. We show loss results significantly reduced specification efficiency an aberrant transcriptome PGC-like (hPGCLCs)...
PRDM14 is a crucial regulator of mouse primordial germ cells (mPGCs), epigenetic reprogramming and pluripotency, but its role in the evolutionarily divergent regulatory network human PGCs (hPGCs) remains unclear. Besides, previous knockdown study indicated that might be dispensable for cell fate. Here, we decided to use inducible degrons more rapid comprehensive depletion. We show loss results significantly reduced specification efficiency an aberrant transcriptome hPGC-like (hPGCLCs)...